year 10, Issue 2 (May - June 2016)                   Iran J Med Microbiol 2016, 10(2): 16-22 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Afshari N, Bakhshi B, Mahmoudi aznaveh A, Fallah F, Rahbar M, Rafiei Tabatabaei S. Investigation of prevalence of Shigella sonnei in children with diarrhea admitted to two hospital Emam Khomeini and Milad in Tehran in 1391 with Antimicrobial susceptibility of isolates. Iran J Med Microbiol 2016; 10 (2) :16-22
1- Islamic Azad University Science & Research Branch, Department of Microbiology, Saveh, Iran
2- Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran ,
3- Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
4- Department of Bacteriology, Pediatric Infection Research Center of Mofid Hospital ,Tehran, Iran
5- Iranian Reference Health Laboratory, Faculty of Medical Microbiology, Tehran, Iran
Abstract:   (12391 Views)

Background and Aim: Shigella infection is one of the prevalent causes of diarrhea disease in most developing countries in children under 10 years old. Conventional microbiological examination to identify Shigella species are time-consuming and requires a lot of work and cost. The object of the present research was to isolate and identify of serotypes of Shigella, Shigella sonnei from patients with bacillary dysentery and to detect their one major virulence genes ipaH by using PCR.

Materials and Methods: 3000 stool sample from children with diarrhea admitted in two famous hospitals in1391 in summer & autumn in Tehran were used in this study. The identification of isolated was done by serogrouping and biochemical test & the prevalence of ipaH gene determined by PCR method using specific pairs of primers. Antimicrobial susceptibility of isolates was performed according to the CLSI guidelines.

Results: All Shigella spp isolates in this study harbored the ipaH gene. Out of 160 shigella isolates, 50 isolates determined as S. sonnei with serogrouping and biochemical test. 90% of isolates were resistant to Tetracycline, Cotrimoxazole, Streptomycin and Minocycline.

Conclusions: We conclude that ipaH PCR procedure is more reliable, sensitive, easier, reproducible and specific which is significantly faster than current conventional detection assays such as serologic test. Althuogh in this study we report that among Shigella .spp cause diarrhea infection most of them are S .sonnei resistant antibiotic.

Full-Text [PDF 496 kb]   (4022 Downloads)    
Type of Study: Original Research Article | Subject: Medical Bacteriology
Received: 2014/11/18 | Accepted: 2015/02/16 | ePublished: 2016/07/24

1. Rajakumar K, Sasakawa C, Adler B. Use of a novel approach, termed island probing, identifies the Shigella flexneri she pathogenicity island which encodes a homolog of the immunoglobulin A protease-like family of proteins.(IAI) Infect and Immuny. 1997;65(11):4606-14. [PubMed]
2. Hale TL. Genetic basis of virulence in Shigella species. Microbiol Mol Biol Rev. 1991;55(2):206-24. [PubMed]
3. Vila J, Gascon J, Abdalla S, Gomez J, Marco F, Moreno A, et al. Antimicrobial resistance of Shigella isolates causing traveler's diarrhea. Antimicrob Agents Chemother. 1994;38(11):2668-70. [PubMed]
4. Shears P. Shigella infections. Ann Trop Med Parasitol. 1996;90(2):105-14.
5. Ranjbar R, Soltan Dallal MM, Talebi M, Pourshafie MR. Increased isolation and characterization of Shigella sonnei obtained from hospitalized children in Tehran, Iran. J Health Popul Nutr. 2008;26(4):426-30. [PubMed]
6. Farshad S, Sheikhi R, Japoni A, Basiri E, Alborzi A. Characterization of Shigella strains in Iran by plasmid profile analysis and PCR amplification of ipa genes. ‎J Clin Microbiol. 2006;44(8):2879-83. [PubMed]
7. Sivapalasingam S, Nelson JM, Joyce K, Hoekstra M, Angulo FJ, Mintz ED. High prevalence of antimicrobial resistance among Shigella isolates in the United States tested by the National Antimicrobial Resistance Monitoring System from 1999 to 2002. Antimicrob Agents Chemother. 2006;50(1):49-54. [PubMed]
8. Faruque SM, Khan R, Kamruzzaman M, Yam Vala MH, Nowroozi J, Ghazi F, Tabatabai PN, Haghighi S. Comparing invasive and non-invasive of isolated Shigella flexneri by electron microscopy of cell culture, SDS-Page and Congo red method. Iran Biomed J. 2007;11(1):47-52. [PubMed]
9. Eftekhari N, Bakhshi B, Pourshafie MR, Zarbakhsh B, Rahbar M, Hajia M, et al. Genetic Diversity of Shigella spp. and Their Integron Content. Foodborne Pathog Dis. 2013;10(3):237-42. [PubMed]
10. Dutta S, Chatterjee A, Dutta P, Rajendran K, Roy S, Pramanik K, et al. Sensitivity and performance characteristics of a direct PCR with stool samples in comparison to conventional techniques for diagnosis of Shigella and enteroinvasive Escherichia coli infection in children with acute diarrhoea in Calcutta, India. J Med Microbiol. 2001;50(8):667-74. [PubMed]
11. Thong KL, Hoe SL, Puthucheary S, Yasin RM. Detection of virulence genes in Malaysian Shigella species by multiplex PCR assay. BMC Infect Dis. 2005;5(1):8. [PubMed]
12. Madiyarov RS, Bektemirov AM, Ibadova GA, Abdukhalilova GK, Khodiev AV, Bodhidatta L, et al. Antimicrobial resistance patterns and prevalence of class 1 and 2 integrons in Shigella flexneri and Shigella sonnei isolated in Uzbekistan. Gut Pathog. 2010;2(1):18. [PubMed]
13. Winn WC, Koneman EW. Koneman's color atlas and textbook of diagnostic microbiology. Lippincott Williams & Wilkins; 2006. [Article]
14. Mirza SH, Beeching NJ, Hart CA. Multi-drug resistant typhoid: a global problem. J Med Microbiol 1996;44(5):317-9. [PubMed]
15. Bhutta ZA, Farooqui BJ, Sturm AW. Eradication of a multiple drug resistant Salmonella paratyphi A causing meningitis with ciprofloxacin. J Infect. 1992;25(2):215-9. [PubMed]
16. Soltandallal MM, Rahimi-Forushani A, Aminharati F, Ohadian-Moghadam s, Nikmanesh B, Rastegare-Lari A. Investigation the Shigella serotypes invasive cells isolated from patients with diarrhea in HEp-2 cell culture. J Shahrekord Univ Med Sci Research. 2013;15(6):100-8. [Article]
17. Asaki S, Ahmad QS, Azim T, et al. Isolation of Shigella dysenteriae type 1 and S. flexneri strains from surface waters in Bangladesh: comparative molecular analysis of environmental Shigella isolates versus clinical strains. Appl Environ Microbiol. 2002;68(8):3908-13. [PubMed]
18. Sethabutr O, Venkatesan M, Yam S, Pang LW, S moak BL, Sang WK, et al. Detection of PCR products of the ipaH gene from Shigella and enteroinvasive Escherichia coli by enzyme linked immunosorbent assay. Diagn Microbiol Infect Dis. 2000;37(1):11-6. [PubMed]
19. Savadkoohi, R.B.; Kacho A.M.Prevalence of Shigella Species and Their Antimicrobial Resistance Patterns at Amirkola Children's Hospital, North of Iran. Iran J Ped. 2007; 17(2): 118-22 [Article]
20. Rahbar M, Deldari M, Hajia M. Changing prevalence and antibiotic susceptibility patterns of different Shigella species in Tehran, Iran. The Internet J Microbiol. 2007;3(2). [Article]
21. Mirnejad R, Vahdati AR, Rashidiani J, Erfani M, Piranfar V. The antimicrobial effect of lactobacillus casei culture supernatant against multiple drug resistant clinical isolates of Shigella sonnei and Shigella flexneri in vitro. Iran Red Crescent Med J. 2013;15(2):122-6. [PubMed]
22. Nowroozi J. Plasmid profile, antibiotic resistance, and phenotypic virulent strains of S. flexneri. Iranian J Pub Health. 2006;35(4):43-8. [Article]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2023 CC BY-NC 4.0 | Iranian Journal of Medical Microbiology

Designed & Developed by : Yektaweb | Publisher: Farname Inc