Investigation of prevalence of Shigella sonnei in children with diarrhea admitted to two hospital Emam Khomeini and Milad in Tehran in 1391 with Antimicrobial susceptibility of isolates

Afshari, Nasim; Bakhshi, Bita; Mahmoudi aznaveh, Azam; Fallah, Fatemeh; Rahbar, Mohammad; Rafiei Tabatabaei, Sedigheh

year 10, Issue 2 (May - June 2016) Iran J Med Microbiol 2016, 10(2): 16-22 | Back to browse issues page

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Afshari N, Bakhshi B, Mahmoudi aznaveh A, Fallah F, Rahbar M, Rafiei Tabatabaei S. Investigation of prevalence of Shigella sonnei in children with diarrhea admitted to two hospital Emam Khomeini and Milad in Tehran in 1391 with Antimicrobial susceptibility of isolates. Iran J Med Microbiol 2016; 10 (2) :16-22
URL: http://ijmm.ir/article-1-380-en.html

Investigation of prevalence of Shigella sonnei in children with diarrhea admitted to two hospital Emam Khomeini and Milad in Tehran in 1391 with Antimicrobial susceptibility of isolates

Nasim Afshari¹

, Bita Bakhshi

², Azam Mahmoudi aznaveh³

, Fatemeh Fallah⁴

, Mohammad Rahbar⁵

, Sedigheh Rafiei Tabatabaei⁴

1- Islamic Azad University Science & Research Branch, Department of Microbiology, Saveh, Iran
2- Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran , b.bakhshi@modares.ac.ir
3- Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
4- Department of Bacteriology, Pediatric Infection Research Center of Mofid Hospital ,Tehran, Iran
5- Iranian Reference Health Laboratory, Faculty of Medical Microbiology, Tehran, Iran

Abstract: (13112 Views)

Background and Aim: Shigella infection is one of the prevalent causes of diarrhea disease in most developing countries in children under 10 years old. Conventional microbiological examination to identify Shigella species are time-consuming and requires a lot of work and cost. The object of the present research was to isolate and identify of serotypes of Shigella, Shigella sonnei from patients with bacillary dysentery and to detect their one major virulence genes ipaH by using PCR.

Materials and Methods: 3000 stool sample from children with diarrhea admitted in two famous hospitals in1391 in summer & autumn in Tehran were used in this study. The identification of isolated was done by serogrouping and biochemical test & the prevalence of ipaH gene determined by PCR method using specific pairs of primers. Antimicrobial susceptibility of isolates was performed according to the CLSI guidelines.

Results: All Shigella spp isolates in this study harbored the ipaH gene. Out of 160 shigella isolates, 50 isolates determined as S. sonnei with serogrouping and biochemical test. 90% of isolates were resistant to Tetracycline, Cotrimoxazole, Streptomycin and Minocycline.

Conclusions: We conclude that ipaH PCR procedure is more reliable, sensitive, easier, reproducible and specific which is significantly faster than current conventional detection assays such as serologic test. Althuogh in this study we report that among Shigella .spp cause diarrhea infection most of them are S .sonnei resistant antibiotic.

Keywords: Shigell sonnei, Antibiotic Suspectibility, Pediatric patients

Full-Text [PDF 496 kb] (4292 Downloads)

Type of Study: Original Research Article | Subject: Medical Bacteriology
Received: 2014/11/18 | Accepted: 2015/02/16 | ePublished: 2016/07/24

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