year 5, Issue 4 (Winter 2012)                   Iran J Med Microbiol 2012, 5(4): 29-34 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Shokri R, Tabaraei B, Hatami S, Nilchizade Rahbar O. Rapid identification of Salmonella in dairy products by P-CEIA. Iran J Med Microbiol. 2012; 5 (4) :29-34
URL: http://ijmm.ir/article-1-205-en.html
1- Biopharmaceutical production quality supervisor at Pasteur Institute of Iran. , shokrei@gmail.com‬‬
2- Biopharmaceutical production quality supervisor at Pasteur Institute of Iran.
Abstract:   (8944 Views)
Background and Objectives: The aim of this study is to use P-CEIA (Polymixin-Coated Polyester Cloth in EIA) method for rapid isolation of salmonella in dairy products. While other methods such as bacterial culturing and ELISA (ab-EIA) are time consuming and expensive, P-CEIA has been utilized for this study.
Materials and Methods: Different dilutions of S.typhimorium Ra-30 in Buffered peptone water (BPW, pH=7.4) and also ten samples (milk and butterfat) have been studied by two P-CEIA and ab-EIA methods.
Results: Analyzing of findings indicates that by using the standard strains of S.typhimorium Ra-30, sensitivity for P-CEIA method was 106 cfu/mL while ab-EIA was estimated 105 cfu/mL. This sensitivity was balanced after heat treatment by using the sodium deoxy -cholate as a detergent five and three positive results were allocated to unknown milk and butterfat samples which were tested with Ab-EIA and also six and three positive results were allocated to unknown milk and butterfat samples which were tested with P-CEIA. In addition to the P-CEIA method requires 14 hour process time less than ab -EIA method.
Conclusion: Analyzing of findings indicates that P-CEIA method is more privileged cause of its firm-inexpensive and stable process for Salmonella detection in dairy products.
Full-Text [PDF 305 kb]   (2086 Downloads)    
Type of Study: Original | Subject: Medical Bacteriology
Received: 2014/01/3 | Accepted: 2014/01/3 | ePublished: 2014/01/3

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


© 2021 All Rights Reserved | Iranian Journal of Medical Microbiology

Designed & Developed by : Yektaweb | Publisher: Farname Inc