year 5, Issue 1 And 2 (6-2011)                   Iran J Med Microbiol 2011, 5(1 And 2): 28-33 | Back to browse issues page

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Ghajar Kohestani M, Alipour M, Rezaee A. Cloning & Expression of gene for the vitamin B12 receptor in E.coli O157:H7. Iran J Med Microbiol. 2011; 5 (1 and 2) :28-33
URL: http://ijmm.ir/article-1-178-en.html
1- , Maryam.Qajar@yahoo.com
Abstract:   (12632 Views)
Background and objectives: Producing and recognition Vitamin B12 transmitter protein from Ecoli O157.H7
Material and Methods: btuB gene of Ecoli O157.H7 by using PCR proliferation and in pET28 a (t) vector cloned. resulted structure confirmed by means of limiting Anzyme Analysis and DNA sequence determination. pET28a(+) – btuB transformed in Ecoli Bl21 and recombinant btuB experission with His-Taq C-terminal performed successfully.
Results: a 66 kiloDoulton Band observed in gell which indicated BtuB protein.
Conclusion: Proliferation btuB gene and producing of BtuB protein with molecular weight 66 kilo Dalton
Full-Text [PDF 282 kb]   (4652 Downloads)    
Type of Study: Original | Subject: Microbial Biotechnology
Received: 2013/11/30 | Accepted: 2013/11/30 | ePublished: 2013/11/30

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