year 15, Issue 2 (March-April 2021)                   Iran J Med Microbiol 2021, 15(2): 189-194 | Back to browse issues page


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Jabin T, Siam E A, Dipu M R, Asaduzzaman M, Uddin A. Phenotypic characterization of Salmonella typhi from clinical specimens and determination of the drug susceptibility pattern of the isolates. Iran J Med Microbiol. 2021; 15 (2) :189-194
URL: http://ijmm.ir/article-1-1139-en.html
1- Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh.
2- Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh. , aftab_mb12@stamforduniversity.edu.bd
Abstract:   (452 Views)

Background and Objective: Salmonella typhi infection occurs in most endemic areas. Patients suspected of typhoid fever located in the Badda and Shantinagar localities of Dhaka were the studied population. A total of 107 blood specimens were collected.
This study attempted to determine the antibiotic susceptibility pattern of Salmonella typhi among different age and gender groups having clinical symptoms of typhoid fever.
Materials and Methods: The microorganisms obtained from blood specimens were identified by phenotypical and biochemical tests following standard protocols. Antibiotic susceptibility test was done by the Agar-disc-diffusion method to understand antibiotic susceptibility of the isolates. All the Salmonella typhi isolates were serologically confirmed using a commercial antisera kit.
Results and Conclusion: Out of 107, 74 Salmonella typhi isolates showed multidrug-resistant properties. Nalidixic acid, ampicillin, azithromycin, and ciprofloxacin showed high antibiotic resistance percentage. On the other hand meropenem, amikacin, gentamycin showed low antibiotic resistance. Age and sex groups also revealed significant attributes to the multidrug-resistant isolates. Multidrug-resistant pattern of the clinical isolates of Salmonella typhi of the current study has presented a matter of great public health concern in Dhaka city, Bangladesh. The first line of antibiotics can be prescribed, taking into consideration of age and gender. The sources of Salmonella typhi infection and mode of transmission must be identified.

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Type of Study: Original | Subject: Antibiotic Resistance
Received: 2020/05/13 | Accepted: 2020/12/28 | ePublished: 2021/04/9

References
1. Selvaraj R, Das R, Ganguly S, Ganguli M, Dhanalakshmi S, Mukhopadhayay SK. Characterization and antibiogram of Salmonella spp. from poultry specimens. J Microbiol Antimicrob. 2010; 2(9): 123-6.
2. Bhan MK, Bahl R, Bhatnagar S. Typhoid and paratyphoid fever. The Lancet. 2005; 366(9487):749-62. [DOI:10.1016/S0140-6736(05)67181-4]
3. Crump JA, Luby SP, Mintz ED. The global burden of typhoid fever. Bull World Health Organ. 2004; 82(5): 346-53.
4. Cabral JPS. Water Microbiology. Bacterial Pathogens and Water. Int J Environ Res Public Health. 2010; 201(7): 3657-703. [DOI:10.3390/ijerph7103657] [PMID] [PMCID]
5. Zhang S, Kingsley R. A, Santos RL, Andrews-Polymenis H, Raffatellu M, Figueiredo J, et al. Molecular pathogenesis of Salmonella enterica serotype typhimurium induced diarrhea. Infect. Immun. 2003; 71(1):1-12. [DOI:10.1128/IAI.71.1.1-12.2003] [PMID] [PMCID]
6. Nagshetty K, Channappa ST, Gaddad SM. Antimicrobial susceptibility of Salmonella typhi in India. J Infect Dev Countries. 2010; 4(2):70-3. [DOI:10.3855/jidc.109] [PMID]
7. Rahman MA. Antimicrobial Resistance Patterns of Salmonella Typhi Isolated from Stool Culture. Chattagram Maa-O-Shishu Hosp Med Coll J. 2015; 14(1):26-30. [DOI:10.3329/cmoshmcj.v14i1.22876]
8. Ammah A, Nkuo-akenji T, Ndip R, Deas JE. An update of concurrent malaria and typhoid fever in Cameroon. Trans. R. Soc. Trop. Med. Hyg. T ROY SOC TROP MED H. 1999; 93:127-9. [DOI:10.1016/S0035-9203(99)90282-1]
9. Kreig NR., Holt JG, Bergey DH. Bergeys Manual of Systematic Bacteriology. 1st ed. East Preston street, Baltimore; 1984.
10. Collee JG, Marmion BP, Fraser AG. Practical Medical Microbiology. 14th ed. Churchill Livingstone, New York; 1996.
11. Cheesbrough M. Medical Laboratory Manual for Tropical Countries. Vol 2. Cambridgeshire (UK); 1984.
12. Bauer A.W, Kirby W.M.M, Sheris J.C., Truck M. Antibiotic susceptibility testing by a standardized single disc method. Am J Clin Path 1966; 145:225-30.
13. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing; Seventeenth Information Supplement, CLSI document M100-S17. Clinical and Laboratory Standards Institute, Wayne, Pa, USA; 2011
14. Azmi I, Akter M, HasanTN, Akter F, Sultana H, Banik D, et al. Mechanism of Fluoroquinolone Resistance in Salmonella Typhi. 8th International Conference on Typhoid Fever and Other Invasive Salmonellosis. 2013; 34-5.
15. Rahman AKMM, Ahmad M, Begum RS, Hossain MZ, HO J, Que Sa, et al. Prevalance of typhoid fever among the children in a semi urban area of Bangladesh. J Dhaka Med Coll. 2006; 20(1): 37-43. [DOI:10.3329/jdmc.v20i1.8570]
16. Mannan A. A cross sectional study on antibiotic resistance pattern of Salmonella Typhi clinical isolates from Bangladesh. Asian Pac J Trop Biomed. 2014; 4(4): 306-11. [DOI:10.12980/APJTB.4.2014C770] [PMID] [PMCID]
17. Ahmed D, D'Costa LT, Alam K, Nair GB, Hossain MA. Multidrug-resistant Salmonella enterica serovar typhi isolates with high-level resistance to ciprofloxacin in Dhaka, Bangladesh. Antimicrob Agents Chemother. 2006; 50:3516-7. [DOI:10.1128/AAC.00667-06] [PMID] [PMCID]
18. Akter L, Hassan M, Ahmed Z. Present status and antibiotic sensitivity pattern of Salmonella typhi and S. paratyphi in different age group hospitalized patients in Dhaka city, Bangladesh. IOSR J Pharm Biol Sci. 2012; 4:27-30. [DOI:10.9790/3008-0432730]
19. Madhulika U, Harish BN, Pajra SC. Current pattern in antimicrobial susceptibility of Salmonella typhi isolates in Pondicherry. Ind J Med. Res. 2004; 120(2):111-4.
20. Hernández JR, Martínez-Martínez L, Cantón R, Coque TM, Pascual A, the Spanish Group for Nosocomial Infections (GEIH). Nationwide study of Escherichia coli and Klebsiella pneumoniae producing extended-spectrum -lactamases in Spain. Antimicrob. Agents Chemother. 2005; 49: 2122-5.

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