year 14, Issue 1 (January - February 2020)
Iran J Med Microbiol 2020, 14(1): 44-54 |
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Mahmoudi H, Zare Fahim N, Alikhani M Y, Shokoohizadeh L. Investigation of Antimicrobial Effect of Berberine on Ciprofloxacin and Imipenem Resistance Acinetobacter baumannii Isolated from Hamadan Hospitals. Iran J Med Microbiol 2020; 14 (1) :44-54
URL: http://ijmm.ir/article-1-957-en.html
URL: http://ijmm.ir/article-1-957-en.html
1- Student Research Committee, Hamadan University of Medical Sciences, Hamadan, Iran
2- Department of Medical Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
3- Department of Medical Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran ,leilishokoohizadeh@gmail.com
2- Department of Medical Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
3- Department of Medical Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran ,
Abstract: (6875 Views)
Background: Acinetobacter baumannii is one of the causes of nosocomial infections, especially in the intensive care unit. The emergence of multidrug-resistant strains of A. baumannii has caused many problems. One of the ways to handle the phenomenon of antibiotic resistance is the use of herbal medicines and their derivatives in place of or in combination with antibiotics. The aim of this study was to investigate the effect of inhibitory effects of berberine as a barberry derivative on clinical isolates of A. baumannii, resistant to ciprofloxacin and imipenem in Hamadan hospitals.
Materials and Methods: In this study, 70 clinical isolates of A. baumannii. were identified and diagnosed using conventional microbiology. Resistance of isolates was detected against imipenem and ciprofloxacin by disk diffusion and broth microdilution method. Minimum inhibitory concentration (MIC) of berberine as well as its combined effect with antibiotics were performed using broth microdilution method.
Results: The results of this study showed that more than 90% of isolates are resistant to ciprofloxacin and imipenem. Imipenem and ciprofloxacin MICs were dtermined from 8 to 28 and 4 to 32 μg / mL, respectively. The berberine decreased the imipenem and ciprofloxacin MIC from zero to two fold and zero to one-fold, respectively
Conclusion: High level resistance to imipenem and ciprofloxacin among A.baumannii isolates is cause of concern. Berberin, in combination with imipenem and ciprofloxacin, reduces MIC to a proper level, which can be used as an effective agent to reduce antibiotic resistance in bacteria.
Materials and Methods: In this study, 70 clinical isolates of A. baumannii. were identified and diagnosed using conventional microbiology. Resistance of isolates was detected against imipenem and ciprofloxacin by disk diffusion and broth microdilution method. Minimum inhibitory concentration (MIC) of berberine as well as its combined effect with antibiotics were performed using broth microdilution method.
Results: The results of this study showed that more than 90% of isolates are resistant to ciprofloxacin and imipenem. Imipenem and ciprofloxacin MICs were dtermined from 8 to 28 and 4 to 32 μg / mL, respectively. The berberine decreased the imipenem and ciprofloxacin MIC from zero to two fold and zero to one-fold, respectively
Conclusion: High level resistance to imipenem and ciprofloxacin among A.baumannii isolates is cause of concern. Berberin, in combination with imipenem and ciprofloxacin, reduces MIC to a proper level, which can be used as an effective agent to reduce antibiotic resistance in bacteria.
Type of Study: Original Research Article |
Subject:
Antimicrobial Substances
Received: 2019/08/18 | Accepted: 2019/12/30 | ePublished: 2020/03/14
Received: 2019/08/18 | Accepted: 2019/12/30 | ePublished: 2020/03/14
References
1. Navidinia M. The clinical importance of emerging ESKAPE pathogens in nosocomial infections. Archives of Advanced in Bioscience. 2016;7(3):43-57.
2. Gao WW, Gopala L, Bheemanaboina RRY, Zhang GB, Li S, Zhou CH. Discovery of 2-aminothiazolyl berberine derivatives as effectively antibacterial agents toward clinically drug-resistant Gram-negative Acinetobacter baumanii. European journal of medicinal chemistry. 2018;146:15-37. [DOI:10.1016/j.ejmech.2018.01.038] [PMID]
3. Jung J, Park W. Acinetobacter species as model microorganisms in environmental microbiology: current state and perspectives. Applied microbiology and biotechnology. 2015;99(6):2533-48. [DOI:10.1007/s00253-015-6439-y] [PMID]
4. Winn WC, Allen SD, Janda WM, Koneman EW, Procop GW, Schreckenberger PC, et al. Taxonomy, biochemical characteristics and clinical significance of medically important nonfermenters. In: Darcy P, Peterson N, editors. Koneman's Colour Atlas and Textbook of Diagnostic Microbiology. 6th ed. Philadelphia: Lippincott Williams and Wilkins; 2006. pp. 353-5
5. Makvandi M, Shokoohizadeh L. Antibacterial and Drug Synergistic Activities of Mentha longifolia Essential Oil Against Shigella flexneri and Shigella sonnei. Int J Enteric Pathog. 2017;5(3):92-5. [DOI:10.15171/ijep.2017.21]
6. Clinical and Laboratory Standards Institute [CLSI] CaL, Standards Institute. : Twenty seventh Informational Supplement M100eS27 CLSI C, Wayne P. Performance Standards for Antimicrobial Susceptibility
7. Oddo A, Thomsen TT, Kjelstrup S, Gorey C, Franzyk H, Frimodt-Møller N, et al. An amphipathic undecapeptide with all d-amino acids shows promising activity against colistin-resistant strains of acinetobacter baumannii and a dual mode of action. 2016;60(1):592-9. [DOI:10.1128/AAC.01966-15] [PMID] [PMCID]
8. Cherkaoui A, Emonet S, Renzi G, Schrenzel J. Characteristics of multidrug-resistant Acinetobacter baumannii strains isolated in Geneva during colonization or infection. Annals of clinical microbiology and antimicrobials. 2015;14:42-. [DOI:10.1186/s12941-015-0103-3] [PMID] [PMCID]
9. El-Saed A, Balkhy HH, Al-Dorzi HM, Khan R, Rishu AH, Arabi YM. Acinetobacter is the most common pathogen associated with late-onset and recurrent ventilator-associated pneumonia in an adult intensive care unit in Saudi Arabia. International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases. 2013;17(9):e696-701. [DOI:10.1016/j.ijid.2013.02.004] [PMID]
10. Ebrahimi M, Khansari-nejad B, Ghaznavi-Rad E. High Frequency of Ventilator Associated Pneumonia Nosocomial Co-Infection Causedby Methicillin Resistant Staphylococcusaureus and Carbapenem Resistant Acinetobacter baumannii in Intensive Care Unit. JOURNAL OF IRANIAN CLINICAL RESEARCH. 2015;1:67-71.
11. El-Saed A, Balkhy HH, Al-Dorzi HM, Khan R, Rishu AH, Arabi YMJIJoID. Acinetobacter is the most common pathogen associated with late-onset and recurrent ventilator-associated pneumonia in an adult intensive care unit in Saudi Arabia. 2013;17(9):e696-e701. [DOI:10.1016/j.ijid.2013.02.004] [PMID]
12. Safari M, Mozaffari Nejad AS, Bahador A, Jafari R, Alikhani MY. Prevalence of ESBL and MBL encoding genes in Acinetobacter baumannii strains isolated from patients of intensive care units (ICU). Saudi journal of biological sciences. 2015;22(4):424-9. [DOI:10.1016/j.sjbs.2015.01.004] [PMID] [PMCID]
13. Vahdani P, Yaghoubi T, Aminzadeh Z. Hospital acquired antibiotic-resistant Acinetobacter baumannii infections in a 400-bed hospital in Tehran, Iran. International journal of preventive medicine. 2011 Jul;2(3):127.
14. Shahcheraghi F, Abbasalipour M, Feizabadi MM, Ebrahimipour GH, Akbari N. Isolation and genetic characterization of metallo-β-lactamase and carbapenamase producing strains of Acinetobacter baumannii from patients at Tehran hospitals. Iranian journal of microbiology. 2011 Jun;3(2):68.
15. Baran G, Erbay A, Bodur H, Öngürü P, Akıncı E, Balaban N, Çevik MA. Risk factors for nosocomial imipenem-resistant Acinetobacter baumannii infections. International Journal of Infectious Diseases. 2008;12(1):16-21. [DOI:10.1016/j.ijid.2007.03.005] [PMID]
16. Vazirzadeh J, Behshood P, Heidari L, Ghajav H. Frequency of metallo-β-lactamase and antimicrobial resistance patterns of Acinetobacter baumannii in carbapenem-resistant isolates in intensive care units. Journal of Isfahan Medical School. 2015;32(312):2094-103.
17. Aghayan SS, Mogadam HK, Fazli M, Darban-Sarokhalil D, Khoramrooz SS, Jabalameli F, Yaslianifard S, Mirzaii M. The effects of berberine and palmatine on efflux pumps inhibition with different gene patterns in Pseudomonas Aeruginosa isolated from burn infections. Avicenna journal of medical biotechnology. 2017 Jan;9(1):2.
18. Musumeci R, Speciale A, Costanzo R, Annino A, Ragusa S, Rapisarda A, Pappalardo MS, Iauk L. Berberis aetnensis C. Presl. extracts: antimicrobial properties and interaction with ciprofloxacin. International journal of antimicrobial agents. 2003 Jul 1;22(1):48-53. [DOI:10.1016/S0924-8579(03)00085-2]
19. Wojtyczka R, Dziedzic A, Kępa M, Kubina R, Kabała-Dzik A, Mularz T, Idzik D. Berberine enhances the antibacterial activity of selected antibiotics against coagulase-negative Staphylococcus strains in vitro. Molecules. 2014;19(5):6583-96. [DOI:10.3390/molecules19056583] [PMID] [PMCID]
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