year 13, Issue 2 (May - June 2019)                   Iran J Med Microbiol 2019, 13(2): 137-141 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Yousefi E, Ghouchannezhad Nournia B, Yousefi A, Fakour F. Identification of Escherichia coli O157: H7 from Slaughtered Beef in Mashhad Using Biochemical and Molecular Methods. Iran J Med Microbiol 2019; 13 (2) :137-141
1- Department of Biology, Faculty of Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran
2- Department of Agrotechnology, Faculty of Agriculture, Ferdowsi University, Mashhad, Iran
3- Department of Biology, Faculty of Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran ,
Abstract:   (6193 Views)
Background and Aims: Escherichia coli O157:H7 is one of the main causes of transmitted diseases by food, including meat and meat products. The purpose of this study was to investigate the prevalence of E. coli O157:H7 contamination in Mashhad with the detection of rfbE gene.
Materials and Methods: For this study, 148 slaughtered beef samples from Mashhad (spring to winter, 2018) were randomly collected and transferred to the laboratory. The identification of E. coli O157:H7 was performed by biochemical methods and rfbE gene was detected by PCR colony method.
Results & Discussion: Based on biochemical tests, E. coli O157:H7 was isolated in 7 (4.73%) samples of slaughtered beef and after PCR colony; only 2 (1.35%) of these 7 samples were approved.
According to the results of this study, it can be concluded that beef can be considered as a major reservoir for E. coli O157:H7 and there is a potential for transmission of this pathogen to humans through the consumption of beef and its products. Also, the specific primers of rfbE gene were able to differentiate the E. coli O157:H7 colonies from other cultured colonies on the culture medium during the PCR process. The present research was performed for the first time in the city of Mashhad.
Full-Text [PDF 757 kb]   (1834 Downloads)    
Type of Study: Original Research Article | Subject: Food Microbiology
Received: 2019/05/9 | Accepted: 2019/08/5 | ePublished: 2019/09/15

1. Hiko A, Asrat D, Zewde G. Occurrence of Escherichia coli O157:H7 in retail raw meat products in Ethiopia. J Infect Dev Countries. 2008; 2(5):389-93. [DOI:10.3855/jidc.203]
2. Johnson-Henry KC, Donato KA, Shen-Tu G, Gordanpour, MandSherman P M. Lactobacillus rhamnosus strain GG prevents Enterohemorrhagic Escherichia coli O157:H7-induced changes in epithelial barrier function. Infect Immun. 2008; 76(4):1340-48. [DOI:10.1128/IAI.00778-07] [PMID] [PMCID]
3. Vimont A, Vernozy-Rozand C, Montet MP, Lazizzera C, Bavai C, Delignette-Muller ML. Modeling and predicting the simultaneous growth of Escherichia coli O157:H7 and ground beef background microflora for various enrichment protocols. Appl Environ Microbiol. 2006; 72(1):261-8. [DOI:10.1128/AEM.72.1.261-268.2006] [PMID] [PMCID]
4. Elder RO, Keen JE, Siragusa GR, Barkocy-Gallagher GA, Koohmaraie M, Laegreid WW. Correlation of enterohaemorrhagic Escherichia coli O157 prevalence in faeces, hides and carcasses of beef cattle during processing. PNAS. 2000; 97:2999-3003. [DOI:10.1073/pnas.97.7.2999] [PMID]
5. Heuvelink AE, Zwartkruis-Nahuis JTM, Beumer RR, De Boer E. Occurrence and survival of verocytotoxin-producing Escherichia coli O157 in meats obtained from retail outlets in The Netherlands. J Food Prot. 1999; 62(10):1115-22. [DOI:10.4315/0362-028X-62.10.1115] [PMID]
6. Adeli Z, Firoozeh F, Zibaei M, Shakib P. Prevalence of Shiga toxin and Intimine genes in Shiga toxin-producing Escherichia coli isolated from urine samples in Lorestan, Iran. Feyz. 2013; 17(2):188-94.
7. Tarr PI, Schoening LM, Yea YL, Ward TR, Jelacic S, Whittam TS. Acquisition of the rfb-gnd Cluster in Evolution of Escherichia coli O55 and O157. J Bacteriol. 2000; 182(21):6183-91. [DOI:10.1128/JB.182.21.6183-6191.2000] [PMID] [PMCID]
8. Tarr PI, Schoening LM, Yea YL, Ward TR, Jelacic S, Whittam TS. Acquisition of the rfb-gnd cluster in evolution of Escherichia coli O55 and O157. J Bacteriol. 2000; 182(21):6183-91. [DOI:10.1128/JB.182.21.6183-6191.2000] [PMID] [PMCID]
9. Byrne CM, Erol I, Call JE, Kaspar CW, Buege DR, Hiemke CJ, et al. Characterization of Escherichia coli O157:H7 from downer and healthy dairy cattle in the upper Midwest region of the United States. Appl Environ Microbiol. 2003; 69(8):4683-8. [DOI:10.1128/AEM.69.8.4683-4688.2003] [PMID] [PMCID]
10. Murphy BP, McCabe E, Murphy M, Buckley JF, Crowley D, Fanning S, et al. Longitudinal Study of Two Irish Dairy Herds: Low Numbers of Shiga Toxin-Producing Escherichia coli O157 and O26 Super-Shedders Identified. Front Microbiol. 2016; 7:1850. [DOI:10.3389/fmicb.2016.01850] [PMID] [PMCID]
11. Shekarfroush S, Tahamtan Y, Pourbakhsh A. Detection and frequency of Stx2 gene in Escherichia coli O157 and O157:H7 strains isolated from sheep carcasses in Shiraz-Iran. Pak J Biol Sci. 2008; 11(8):1085-92. [DOI:10.3923/pjbs.2008.1085.1092] [PMID]
12. Rahimi E, Momtaz H, Hemmatzadeh F. The prevalence of Escherichia coli O157:H7, Listeria monocytogenes and Campylobacter spp. on bovine carcasses in Isfahan, Iran. IJVR. 2008; 9(4):365-70.
13. Mousavi SL, Rasooli I, Nazarian S, Amani J. Simultaneous detection of Escherichia coli O157:H7, toxigenic Vibrio cholerae, and Salmonella typhimurium by multiplex PCR. IJCID. 2009; 4(2):97-103.
14. Fode-Vaughan KA, Maki JS, Benson JA, Collins ML. Direct PCR detection of Escherichia coli O157: H7. Lett Appl Microbiol. 2003; 37(3):239-43. [DOI:10.1046/j.1472-765X.2003.01386.x] [PMID]
15. Campbell GR, Prosser J, Glover A, Killham K. Detection of Escherichia coli O157:H7 in soil and water using multiplex PCR. J Appl Microbiol. 2001; 91(6):1004-10. [DOI:10.1046/j.1365-2672.2001.01465.x] [PMID]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Iranian Journal of Medical Microbiology

Designed & Developed by : Yektaweb | Publisher: Farname Inc