year 1, Issue 2 (Summer 2007)
Iran J Med Microbiol 2007, 1(2): 1-8 |
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Bakhtiari R, Soltan Dallal M, Zaemi Yazdi J, Fallah J, Amir Mozaffari N, Pourmand M et al . Evaluation of PCR method for diagnosis of Group B Streptococcuscarriage in pregnant women. Iran J Med Microbiol 2007; 1 (2) :1-8
URL: http://ijmm.ir/article-1-78-en.html
URL: http://ijmm.ir/article-1-78-en.html
Ronak Bakhtiari1 
, Mohhamad Soltan Dallal2 , Javad Zaemi Yazdi3 , Jalil Fallah4 , Nour Amir Mozaffari5 , Mohhamad Pourmand2 , Sara Hajikhani2
, Mohhamad Soltan Dallal2 , Javad Zaemi Yazdi3 , Jalil Fallah4 , Nour Amir Mozaffari5 , Mohhamad Pourmand2 , Sara Hajikhani2
1- Science and Research Compose Islamic Azad University, Tehran
2- Department of Pathobiology, School of Public Health, Medical Sciences/University of Tehran
3- Department of Pathobiology, School of Medicine,Yazd Shaid Sadoughi, Yazd University of Medical Sciences, Yazd
4- Modern Age Bioinformatics Institute, Tehran
5- Department of Microbiology, School of Medicine, Iran University of Medical Sciences
2- Department of Pathobiology, School of Public Health, Medical Sciences/University of Tehran
3- Department of Pathobiology, School of Medicine,Yazd Shaid Sadoughi, Yazd University of Medical Sciences, Yazd
4- Modern Age Bioinformatics Institute, Tehran
5- Department of Microbiology, School of Medicine, Iran University of Medical Sciences
Abstract: (16854 Views)
Background and Objectives: Group B Streptococcus (GBS) (Streptococcus agalactiae) is the leading cause
of morbidity and mortality of newborn infants and accounted as a leading factor causing septicemia after
birth in mothers. Infections in infants are usually acquired by contact with the genital tract of the mothers
during labor and delivery. In two last decades, significant progress toward detection, prevention and
treatment of pregnant women carrying GBS has been achieved. A rapid screening test for GBS that could
accurately identify pregnant women carrying the bacteria at the time of delivery would obviate the need for
prenatal screening.
The standard method for the diagnosis of GBS colonization consists of culturing vaginal and anal secretions
in a selective broth medium which inhibits the growth of other microorganisms. Today, it is accepted that
PCR has a high sensitivity and specifically in diagnosis. The goal of this study was to screen pregnant
woman carrying GBS by PCR.
Material and Methods: Samples were taken from anal and vaginal mucus of 125 pregnant women who were at 28-38 weeks of ingestion by swab. Samples were tested by standard culture using Todd Hewitt Broth and Blood Agar and also by PCR using primers specific for cfbgene.
Results: Culture identified 10 (8%) women as carriage of GBSout of 125 women tested. On the other hand, the PCR assay could identify 12 (9/6%) women positive for GBS. In comparison to culture results, sensitivity, NPV, specificity, and PPV of PCR were 100%, 100%, 98%, and 83%, respectively. The time required for PCR assay and culture were 2h and 36h,respectively.
Conclusion: We found that GBS can be detected rapidly and reliably by a PCR assay using combined vaginal and anal secretions from pregnant women at the time of delivery. Also this study shows that the rate of incidence of GBS is high in Iranian pregnant women. We, therefore, recommend screening of pregnant women for detecting of GBS emphatically
Material and Methods: Samples were taken from anal and vaginal mucus of 125 pregnant women who were at 28-38 weeks of ingestion by swab. Samples were tested by standard culture using Todd Hewitt Broth and Blood Agar and also by PCR using primers specific for cfbgene.
Results: Culture identified 10 (8%) women as carriage of GBSout of 125 women tested. On the other hand, the PCR assay could identify 12 (9/6%) women positive for GBS. In comparison to culture results, sensitivity, NPV, specificity, and PPV of PCR were 100%, 100%, 98%, and 83%, respectively. The time required for PCR assay and culture were 2h and 36h,respectively.
Conclusion: We found that GBS can be detected rapidly and reliably by a PCR assay using combined vaginal and anal secretions from pregnant women at the time of delivery. Also this study shows that the rate of incidence of GBS is high in Iranian pregnant women. We, therefore, recommend screening of pregnant women for detecting of GBS emphatically
Type of Study: Brief Original Article |
Subject:
Molecular Microbiology
Received: 2013/11/10 | Accepted: 2013/11/11 | ePublished: 2013/11/11
Received: 2013/11/10 | Accepted: 2013/11/11 | ePublished: 2013/11/11
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