year 11, Issue 6 (January - February 2018)                   Iran J Med Microbiol 2018, 11(6): 203-209 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Larijanian L, Hashemi J, Siadat D. Identification of Etiologic Agents of Actinomycetal Infections among Patients Referred to the Health Faculty of Tehran University of Medical Sciences . Iran J Med Microbiol. 2018; 11 (6) :203-209
1- Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran ,
2- Department of Medical Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
3- Department of Tuberculosis and Pulmonary Research, Microbiology Research Center, Pasteur Institute of Iran, Tehran, Iran
Abstract:   (5739 Views)

Background and Aims: Actinomycetes are a group of Gram-positive bacteria that have filamentous morphology with about one micron diameter. Actinomyces, Nocardia and Streptomyces are classified in this group. In this study, the etiological agents of actinomycetal infections were determined among patients referred to the Health faculty of Tehran University of Medical Sciences (TUMS).
Materials and Methods: To determine the status of actinomycetes diseases, 465 specimens including the broncho-alveolar lavage (BAL), cerebrospinal fluid (CSF), tissue biopsy, abscess discharge and other clinical materiasl were examined via microscopic observation and culture methods. (2011-2015)
Results and Conclusions: Out of the 465 specimens, 20 (4.3%) were diagnosed as infected which included 11(55% ) Actinomyces and 9 (45% ) Nocardia. The most positive results were associated with pulmonary infections. Among the infected patients, 60 % (12) were male and 40% (8) were female. The results of this study showed that the prevalence of Actinomyces and Nocardia was similar, being more predominant among men compared to women. In order to obtain the appropriate results, it is recommended that samples  are taken during doctor's visit and before starting a treatment. It is also recommended to use molecular methods such as PCR and RT-PCR for better identification and comparison with phenotypic methods.

Full-Text [PDF 689 kb]   (1976 Downloads)    
Type of Study: Brief Original Article | Subject: Medical Bacteriology
Received: 2017/05/4 | Accepted: 2017/10/2 | ePublished: 2018/03/19

1. Cross T, Goodfellow M, editors. Taxonomy and classification of the actinomycetes. Symp Ser Soc Appl Microbiol; 1973.
2. Goodfellow M, Stanton L, Simpson K, Minnikin D. Numerical and chemical classification of Actinoplanes and some related actinomycetes. Microbiology. 1990;136(1):19-36. [DOI]
3. Harz CO. Actinomyces bovis ein neuer Schimmel in den Geweben des Rindes. Duet Ztschr Thiermed. 1878;5:125-40.
4. Richtsmeier WJ, Johns ME, Coulthard SW. Actinomycosis of the head and neck. Crit Rev Clin Lab Sci. 1979;11(2):175-202. [DOI]
5. Waksman SA. On the classification of actinomycetes. Journal of bacteriology. 1940;39(5):549. [PubMed]
6. Lippes J. Pelvic actinomycosis: a review and preliminary look at prevalence. Am J Obstet Gynecol. 1999;180(2):265-9. [DOI]
7. Wong V, Turmezei T, Weston V. Actinomycosis. BMJ: British Medical Journal (Online). 2011;343.
8. Sabbe LJ, Van De Merwe D, Schouls L, Bergmans A, Vaneechoutte M, Vandamme P. Clinical spectrum of infections due to the newly described Actinomyces species A. turicensis, A. radingae, and A. europaeus. J Clin Microbiol. 1999;37(1):8-13. [PubMed]
9. Eshraghi S, Salari M, Kadkhoda Z, Yaghmaei S. Isolation and characterization of oral Actinomyces strain from patients with periodontal disease. J Dent Med Tehran Univ Med Sci. 2001;14(3):21-9.
10. Sudhakar SS, Ross JJ. Short-term treatment of actinomycosis: two cases and a review. Clin Infect Dis. 2004;38(3):444-7. [DOI] [PubMed]
11. Park JK, Lee HK, Ha HK, Choi HY, Choi CG. Cervicofacial actinomycosis: CT and MR imaging findings in seven patients. AJNR Am J Neuroradiol. 2003;24(3):331-5. [PubMed]
12. Castillo UF, Strobel GA, Ford EJ, Hess WM, Porter H, Jensen JB, et al. Munumbicins, wide-spectrum antibiotics produced by Streptomyces NRRL 30562, endophytic on Kennedia nigriscansa. Microbiology. 2002;148(9):2675-85. [DOI] [PubMed]
13. Lawson PA, Falsen E, Åkervall E, Vandamme P, Collins MD. Characterization of some Actinomyces-like isolates from human clinical specimens: reclassification of Actinomyces suis (Soltys and Spratling) as Actinobaculum suis comb. nov. and description of Actinobaculum schaalii sp. nov. Int J Syst Evol Microbiol. 1997;47(3):899-903. [DOI]
14. Couble A, Rodriguez-Nava V, de Montclos MP, Boiron P, Laurent F. Direct detection of Nocardia spp. in clinical samples by a rapid molecular method. J Clin Microbiol. 2005;43(4):1921-4. [DOI] [PubMed]
15. McNeil MM, Brown JM. The medically important aerobic actinomycetes: epidemiology and microbiology. Clin Microbiol Rev. 1994;7(3):357-417. [DOI]
16. Yorke RF, Rouah E. Nocardiosis with brain abscess due to an unusual species, Nocardia transvalensis. Arch Pathol Lab Med. 2003;127(2):224-6. [PubMed]
17. Wagenlehner F, Mohren B, Naber K, Männl H. Abdominal actinomycosis. Clinical microbiology and infection. 2003;9(8):881-5. [DOI] [PubMed]
18. Lee I-J, Ha HK, Park CM, Kim JK, Kim JH, Kim TK, et al. Abdominopelvic actinomycosis involving the gastrointestinal tract: CT features. Radiology. 2001;220(1):76-80. [DOI] [PubMed]
19. Abtahi H, Saffari M, Jourabchi A, Rafiei M. Pulmonary nocardiosis and its related factors in patients with pulmonary infection in Arak. KAUMS Journal (FEYZ). 2003;7(3):87-91.
20. Eshraghi SS, Zomorodian K, Kord Bacheh P, Gerami Shoar M, Saber S. Nocardiosis in pulmonary patients. J Medical Faculty Guilan University of Medical Sciences. 2003;11(44):28-33.
21. Kageyama A, Poonwan N, Yazawa K, Suzuki S-i, Kroppenstedt RM, Mikami Y. Nocardia vermiculata sp. nov. and Nocardia thailandica sp. nov. isolated from clinical specimens. Actinomycetologica. 2004;18(2):27-33. [DOI]
22. Zaini F, Mehbod A, Emami M. Comprehensive medical mycology. Tehran: Tehran University Publication; 1999:39-40.
23. Khan Z, Al-Sayer H, Chugh TD, Chandy R, Provost F, Boiron P. Antimicrobial susceptibility profile of soil isolates of Nocardia asteroides from Kuwait. Clin Microbiol Infect. 2000;6(2):94-8. [DOI] [PubMed]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2022 CC BY-NC 4.0 | Iranian Journal of Medical Microbiology

Designed & Developed by : Yektaweb | Publisher: Farname Inc