year 11, Issue 5 (November - December 2017)                   Iran J Med Microbiol 2017, 11(5): 115-124 | Back to browse issues page

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Abbasvali M, Ebrahimi Kahrizsangi A, Shahriari F. Evaluation of the Inhibitory Effects of Zinc Oxide Nanoparticles on Biofilm Formation of Some Foodborne Bacterial Pathogens . Iran J Med Microbiol. 2017; 11 (5) :115-124
1- Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran ,
2- Department of Pathobiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
3- Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
Abstract:   (6368 Views)

Background and Aims: One of the most important factors in food industry is the formation of microbial biofilm which can be the potential source of food products contamination with food spoilage and foodborne pathogenic bacteria. This study was conducted to evaluate the inhibitory effects of zinc oxide nanoparticles on biofilm formation of some foodborne bacterial pathogens.
Materials and Methods: This research was carried out in 2015. Minimum inhibitory concentration (MIC) of zinc oxide nanoparticles for E. coli (ATCC 35218), Staphylococcus aureus (ATCC 6538), Salmonella typhimurium (ATCC 14028) and Bacillus cereus (ATCC 14579) was determined by using 96-well microplate and resazurin reduction method. Biofilm formation inhibition was assessed with microtiter plate method based on crystal violet staining and measurement of optical density using microplate reader.
Results: MIC of zinc oxide nanoparticles for the above mentioned bacteria was respectively 1, ≤ 0.5, 1 and 2 mg/mL. The inhibition percentages of biofilm formation at the MIC of the nanoparticles were 90.20, 85.69, 83.65 and 61.96, respectively.
Conclusions: In this study, zinc oxide nanoparticle showed the ability to inhibit the formation of biofilm even in sub inhibitory concentrations or Sub-MICs.  No significant difference (P>0.05) was observed in the biofilm inhibitory effects of ZnO nanoparticles at MIC and 1/2×MIC between E. coli and S. aureus. Results of this study showed the inhibitory effects of zinc oxide nanoparticles on biofilm formation of the studied foodborne bacterial pathogens. According to the importance of these bacteria in public health, ZnO nanoparticle can be used as a cleaning agent for the surfaces, apparatus and production lines in food plants in order to prevent foodborne bacterial biofilm formation.

Full-Text [PDF 1227 kb]   (3199 Downloads)    
Type of Study: Original | Subject: Food Microbiology
Received: 2016/10/26 | Accepted: 2017/10/2 | ePublished: 2017/11/20

1. Mah TF, O'Toole GA. Mechanisms of biofilm resistance to antimicrobial agents. Trends Microbiol 2001; 9 (1): 34-9. [DOI]
2. Lear G, Lewis GD, editors. Microbial biofilms: current research and applications. Horizon Scientific Press; 2012.
3. Costerton JW. Overview of microbial biofilms. J Ind Microbiol 1995; 15(3): 137-40. [DOI] [PubMed]
4. Costerton JW, Irvin RT, Cheng KJ. The bacterial glycocalyx in nature and disease. Ann Rev Microbiol 1981; 35 (1): 299-324. [DOI] [PubMed]
5. Parsek MR, Fuqua C. Biofilms 2003: emerging themes and challenges in studies of surface-associated microbial life. J Bacteriol 2004; 186 (14): 4427-40. [DOI] [PubMed]
6. Burt S. Essential oils: their antibacterial properties and potential applications in foods—a review. Int J Food Microbiol 2004; 94 (3): 223-53. [DOI] [PubMed]
7. Bryers JD. BiofilmsII: process analysis and application. Wiley, New York 2000; 327-360.
8. Dunsmore DG, Twomey A, Whittlestone WG, Morgan HW. Design and performance of systems for cleaning product-contact surfaces of food equipment: a review. J Food Protect 1981; 44 (3): 220-40. [DOI]
9. Whitesides GM. Nanoscience, nanotechnology, and chemistry. Small 2005; 1(2):172-9. [DOI] [PubMed]
10. Bogdanović U, Lazić V, Vodnik V, Budimir M, Marković Z, Dimitrijević S. Copper nanoparticles with high antimicrobial activity. Mater Lett 2014; 128: 75-8. [DOI]
11. Beranová J, Seydlová G, Kozak H, Benada O, Fišer R, Artemenko A, Konopásek I, Kromka A. Sensitivity of bacteria to diamond nanoparticles of various size differs in gram-positive and gram-negative cells. FEMS Microbiol Lett 2014; 351 (2):179-86. [DOI] [PubMed]
12. Huh AJ, Kwon YJ. “Nanoantibiotics”: a new paradigm for treating infectious diseases using nanomaterials in the antibiotics resistant era. J Control Release 2011; 156 (2):128-45. [DOI] [PubMed]
13. Sarker SD, Nahar L, Kumarasamy Y. Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods 2007; 42 (4):321-4. [DOI] [PubMed]
14. Tendolkar PM, Baghdayan AS, Gilmore MS, Shankar N. Enterococcal surface protein, Esp, enhances biofilm formation by Enterococcus faecalis. Infect Immun 2004; 72 (10):6032-9. [DOI] [PubMed]
15. Stepanović S, Vuković D, Hola V, Bonaventura GD, Djukić S, Ćirković I, Ruzicka F. Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci. Apmis 2007; 115 (8):891-9. [DOI] [PubMed]
16. Nikolić M, Vasić S, Đurđević J, Stefanović O, Čomić L. Antibacterial and anti-biofilm activity of ginger (Zingiber Officinale (Roscoe)) ethanolic extract. Kragujevac J Sci 2014; 36:129-36. [DOI]
17. Espitia PJ, Soares ND, dos Reis Coimbra JS, de Andrade NJ, Cruz RS, Medeiros EA. Zinc oxide nanoparticles: synthesis, antimicrobial activity and food packaging applications. Food Bioprocess Tech 2012; 5(5):1447-64. [DOI]
18. Esmailzadeh H, Sangpour P, Khaksar R, Shahraz F. The effect of ZnO nanoparticles on the growth of Bacillus subtilis and Escherichia coli O157:H7. J Food Technol Nutr 2014; 11(3): 21-28. [in Persian]
19. Mohammadbeigi P, Sodagar M, Mazandarani M, Hoseini SS. An investigation of antibacterial activity of ZnO nanoparticle on Streptoccocus iniae and Escheria coli. Qom Univ Med Sci J 2016:55-63. [in Persian]
20. Zhang L, Jiang Y, Ding Y, Daskalakis N, Jeuken L, Povey M, O’Neill AJ, York DW. Mechanistic investigation into antibacterial behaviour of suspensions of ZnO nanoparticles against E. coli. J Nanopart Res 2010; 12(5):1625-36. [DOI]
21. Li X, Xing Y, Jiang Y, Ding Y, Li W. Antimicrobial activities of ZnO powder‐coated PVC film to inactivate food pathogens. Int J Food Sci Tech 2009; 44(11):2161-8. [DOI]
22. Pati R, Mehta RK, Mohanty S, Padhi A, Sengupta M, Vaseeharan B, Goswami C, Sonawane A. Topical application of zinc oxide nanoparticles reduces bacterial skin infection in mice and exhibits antibacterial activity by inducing oxidative stress response and cell membrane disintegration in macrophages. Nanomed-Nanotechnol 2014; 10(6):1195-208. [DOI] [PubMed]
23. Emami-Karvani Z, Chehrazi P. Antibacterial activity of ZnO nanoparticle on gram-positive and gram-negative bacteria. Afr J Microbiol Res 2011; 5(12):1368-73.
24. Akiyama H, Yamasaki O, Kanzaki H, Tada J, Arata J. Effects of zinc oxide on the attachment of Staphylococcus aureus strains. J Dermatol Sci 1998; 17(1):67-74. [DOI]
25. Mostafa AA. Antibacterial activity of Zinc oxide nanoparticles against toxigenic Bacillus cereus and Staphylococcus aureus isolated from some Egyptian food. Int J Microbiol Res 2015; 6(2):145-54.
26. Sinha R, Karan R, Sinha A, Khare SK. Interaction and nanotoxic effect of ZnO and Ag nanoparticles on mesophilic and halophilic bacterial cells. Bioresource Technol 2011; 102 (2):1516-20. [DOI] [PubMed]
27. Yousef JM, Danial EN. In vitro antibacterial activity and minimum inhibitory concentration of zinc oxide and nano-particle zinc oxide against pathogenic strains. J Health Sci 2012; 2(4):38-42. [DOI]
28. Wu C, Labrie J, Tremblay YD, Haine D, Mourez M, Jacques M. Zinc as an agent for the prevention of biofilm formation by pathogenic bacteria. J Appl Microbiol 2013;115(1):30-40. [DOI] [PubMed]
29. Lee JH, Kim YG, Cho MH, Lee J. ZnO nanoparticles inhibit Pseudomonas aeruginosa biofilm formation and virulence factor production. Microbiol Res 2014; 169(12):888-96. [DOI] [PubMed]
30. Vijayakumar S, Vinoj G, Malaikozhundan B, Shanthi S, Vaseeharan B. Plectranthus amboinicus leaf extract mediated synthesis of zinc oxide nanoparticles and its control of methicillin resistant Staphylococcus aureus biofilm and blood sucking mosquito larvae. Spectrochim Acta A 2015; 137:886-91. [DOI] [PubMed]
31. Dhillon GS, Kaur S, Brar SK. Facile fabrication and characterization of chitosan-based zinc oxide nanoparticles and evaluation of their antimicrobial and antibiofilm activity. Int Nano Lett 2014; 4(2):1-1. [DOI]
32. Eshed M, Lellouche J, Gedanken A, Banin E. A Zn‐doped CuO nanocomposite shows enhanced antibiofilm and antibacterial activities against streptococcus mutans compared to nanosized CuO. Adv Funct Mater 2014; 24(10):1382-90. [DOI]
33. Akhil K, Jayakumar J, Gayathri G, Khan SS. Effect of various capping agents on photocatalytic, antibacterial and antibiofilm activities of ZnO nanoparticles. J Photoch Photobio B 2016; 160:32-42. [DOI] [PubMed]

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