Discrimination between Invasive and Contaminating  Staphylococcus epidermidis strains according to the multiplex-PCR and study of phenotypic drug resistance

mirzaei, Rasoul; Talebi, Malihe; Mahdiun, Faeze; Foroozeshfard, Mohammad; Irajian, Gholamreza

year 10, Issue 6 (January - February 2017) Iran J Med Microbiol 2017, 10(6): 9-15 | Back to browse issues page

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mirzaei R, Talebi M, Mahdiun F, Foroozeshfard M, Irajian G. Discrimination between Invasive and Contaminating Staphylococcus epidermidis strains according to the multiplex-PCR and study of phenotypic drug resistance. Iran J Med Microbiol 2017; 10 (6) :9-15
URL: http://ijmm.ir/article-1-481-en.html

Discrimination between Invasive and Contaminating Staphylococcus epidermidis strains according to the multiplex-PCR and study of phenotypic drug resistance

Rasoul Mirzaei¹

, Malihe Talebi¹

, Faeze Mahdiun²

, Mohammad Foroozeshfard³

, Gholamreza Irajian

⁴

1- Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
2- Department of Microbiology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
3- Department of Anesthesia, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran
4- Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran , dr.irajian@gmail.com

Abstract: (10006 Views)

Background and Aim: With increase Staphylococcus epidermidis infections, discrimination between invasive and non-invasive S. epidermidis strains and determination of drug resistance, for diagnosis and treatment is important.

Materials and Methods: From August 2014 to March 2015, 123 strains isolated from patients and medical instruments from Ali Asghar and Hazrat Rasoul hospitals were used to discriminate invasive and contaminating S. epidermidis strains by ica, atlE, agrA, sarA, and mecA genes by Multiplex PCR method. Also, according to the CLSI standards, resistance to cefoxitin, linzeolid, clindamycin, erythromycin and tetracycline were tested by disk diffusion method and vancomycin E-test method.

Results: prevalence of ica and mecA genes were 85% and 75% in invasive strains and 20% and 51% in contaminating strains, respectively. The resistance level to cefoxitin, tetracycline, clindamycin and erythromycin antibiotics in invasive strains were: 75℅, 35℅, 37℅, 76℅ and in contaminating strains were; 51℅, 40℅, 30℅, 64℅, respectively. Resistance to vancomycin and linezolid was not observed in this study.

Conclusions: This study shown that ica gene can be used as a suitable marker for discrimination between invasive and contaminating isolates. Also, vancomycin is a better choice for the treatment of resistant patients to methicillin.

KeyWords: Invasive Staphylococcus epidermidis, Methicillin, Disk diffusion, Multiplex PCR

Keywords: Invasive Staphylococcus epidermidis, Methicillin, Disk diffusion, Multiplex PCR

Full-Text [PDF 649 kb] (2559 Downloads)

Type of Study: Original Research Article | Subject: Medical Bacteriology
Received: 2015/09/22 | Accepted: 2016/05/4 | ePublished: 2016/10/17

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