Molecular Characterization of Staphylococcal Cassette Chromosome mec types I to V in Methicillin-resistant Staphylococcus aureus by Multiplex PCR

Umrotkar, Mrinmayee; Gupta , Neetu; Jadhav, Savita Vivekanand

doi:10.30699/ijmm.19.4.254

year 19, Issue 4 (July - August 2025) Iran J Med Microbiol 2025, 19(4): 254-264 | Back to browse issues page

‎ 10.30699/ijmm.19.4.254

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Umrotkar M, Gupta N, Jadhav S V. Molecular Characterization of Staphylococcal Cassette Chromosome mec types I to V in Methicillin-resistant Staphylococcus aureus by Multiplex PCR. Iran J Med Microbiol 2025; 19 (4) :254-264
URL: http://ijmm.ir/article-1-2632-en.html

Molecular Characterization of Staphylococcal Cassette Chromosome mec types I to V in Methicillin-resistant Staphylococcus aureus by Multiplex PCR

Mrinmayee Umrotkar¹

, Neetu Gupta²

, Savita Vivekanand Jadhav³

1- Symbiosis Medical College for Women (SMCW), Symbiosis University Hospital and Research Centre (SUHRC), Symbiosis International (Deemed University), Pune, India
2- Symbiosis Medical College for Women (SMCW), Symbiosis University Hospital and Research Centre (SUHRC), Symbiosis International (Deemed University), Pune, India , neetu.satish13@gmail.com
3- Pacific Medical College and Hospital, Pacific Medical University (PMU), Udaipur, India

Abstract: (500 Views)

Background and Aim: Bacteriocins are antimicrobial peptides produced by bacteria that hold great potential as natural preservatives and bioactive compounds. This study aimed to characterize bacteriocins produced by Bacillus paramycoides strain MCCC 1A04098 (BSH1), Bacillus albus strain MCCC 1A02146 (BSH2), and Bacillus cereus strain IAM 12605 (BSH3), isolated from cincalok, a traditional fermented shrimp product.
Materials and Methods: High-performance liquid chromatography (HPLC) was used to analyze amino acid profiles, and sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) was employed to determine molecular weights.
Results: Bacteriocins from BSH1, BSH2, and BSH3 had molecular weights of 55.02 kDa, 51.15 kDa, and 50.12 kDa, respectively, classifying them as class III bacteriocins (>30 kDa). Amino acid analysis revealed the dominance of L-cystine (33–45%) and L-proline (9–20%), along with notable amounts of glutamic acid and leucine. These amino acids are essential for structural stability and antimicrobial activity.
Conclusion: The findings suggest that bacteriocins derived from cincalok not only broaden the understanding of bacteriocin diversity from local fermented foods but also demonstrate strong potential as natural antimicrobial agents for applications in food preservation and healthcare industries.

Keywords: MRSA, SCCmec typing, multiplex PCR, mecA gene.

Full-Text [PDF 697 kb] (44 Downloads)

Type of Study: Original Research Article | Subject: Molecular Epidemiology
Received: 2025/06/21 | Accepted: 2025/09/19 | ePublished: 2025/10/10

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