year 19, Issue 2 (March - April 2025)
Iran J Med Microbiol 2025, 19(2): 118-126 |
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Hossainpour H, Alvandi A H, Alikhani M Y, Moradi A, Mahmoudi H. Prevalence of Escherichia coli O157:H7 in Clinical and Food Samples: A Cross-sectional Study. Iran J Med Microbiol 2025; 19 (2) :118-126
URL: http://ijmm.ir/article-1-2595-en.html
URL: http://ijmm.ir/article-1-2595-en.html
Hadi Hossainpour1 
, Amir hooshang Alvandi2 , Mohammad Yousef Alikhani3 , Abdollah Moradi4 , Hassan Mahmoudi5
, Amir hooshang Alvandi2 , Mohammad Yousef Alikhani3 , Abdollah Moradi4 , Hassan Mahmoudi5
1- Department of Microbiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran & Zoonoses Research Center, Ardabil University of Medical Sciences, Ardabil, Iran
2- Medical Technology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
3- Infectious Disease Research Center, Avicenna Institute of Clinical Sciences, Hamadan, University of Medical Sciences, Hamadan, Iran & Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
4- Department of Microbiology, Science and Research Branch. Islamic Azad University, Tehran, Iran
5- Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran & Nahavand School of Allied Medical Sciences, Hamadan University of Medical Sciences. Hamadan, Iran ,Hassanmahmoudi24@gmail.com
2- Medical Technology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
3- Infectious Disease Research Center, Avicenna Institute of Clinical Sciences, Hamadan, University of Medical Sciences, Hamadan, Iran & Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
4- Department of Microbiology, Science and Research Branch. Islamic Azad University, Tehran, Iran
5- Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran & Nahavand School of Allied Medical Sciences, Hamadan University of Medical Sciences. Hamadan, Iran ,
Abstract: (144 Views)
Background and Aim: Escherichia (E.) coli O157:H7 is a virulent bacterial strain known for producing a toxin called Shiga toxin, which can damage intestinal and kidney cells leading to bloody diarrhea and kidney failure. Food poisoning is a significant public health issue worldwide. This study aimed to investigate the prevalence of E. coli O157:H7 in clinical and food samples.
Materials and Methods: In this cross-sectional study, 62 food samples, including raw chicken meat, milk, and beef were randomly collected from several supermarkets in different areas of Hamadan City, Iran. Additionally, 62 E. coli strains were collected from various clinical specimens, including wounds, urine, blood, and cerebrospinal fluid, from Besat Hospital in Hamadan, Iran, and analyzed for E. coli O157:H7 contamination. All samples were cultured on specific media, and any suspicious colonies were subjected to PCR for verification of E. coli O157 H7 strain using two target genes.
Results: In total, 2 clinical and 8 food samples were positive for E. coli O157:H7 phenotypically. All isolates were positive for the presence of target genes using PCR. The highest frequency was related to contaminated food, particularly beef, with a frequency of 12.9%.
Conclusion: The results indicated high prevalence of E. coli O157:H7, especially in beef that was higher than the global average. Therefore, it is necessary to implement more precise controls in production and maintenance processes of food samples.
Type of Study: Original Research Article |
Subject:
Food Microbiology
Received: 2025/02/10 | Accepted: 2025/05/28 | ePublished: 2025/06/6
Received: 2025/02/10 | Accepted: 2025/05/28 | ePublished: 2025/06/6
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