The Effect of Bifidobacterium Longum on Expression of Hfq in Escherichia Coli O157:H7

Aboulhassanzadeh, Sobhan; Aboulhassanzadeh, Samin; Aghazadeh, Hamed; Nikkhooy, Zahra; Jafari, Behboud

year 18, Issue 5 (September - October 2024) Iran J Med Microbiol 2024, 18(5): 278-286 | Back to browse issues page

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Aboulhassanzadeh S, Aboulhassanzadeh S, Aghazadeh H, Nikkhooy Z, Jafari B. The Effect of Bifidobacterium Longum on Expression of Hfq in Escherichia Coli O157:H7. Iran J Med Microbiol 2024; 18 (5) :278-286
URL: http://ijmm.ir/article-1-2472-en.html

The Effect of Bifidobacterium Longum on Expression of Hfq in Escherichia Coli O157:H7

Sobhan Aboulhassanzadeh¹

, Samin Aboulhassanzadeh²

, Hamed Aghazadeh³

, Zahra Nikkhooy⁴

, Behboud Jafari⁵

1- School of Biotechnology, Faculty of Science and Health, Dublin City University, Dublin, Ireland & Research Center for Pharmaceutical Nanotechnology (RCPN), Tabriz University of Medical Sciences, Tabriz, Iran , sobhan.aboulhassanzadeh2@mail.dcu.ie
2- Mérieux NutriSciences - Global, Advanced laboratory testing, Newbridge, Ireland & Research Center for Pharmaceutical Nanotechnology (RCPN), Tabriz University of Medical Sciences, Tabriz, Iran
3- Research Center for Pharmaceutical Nanotechnology (RCPN), Tabriz University of Medical Sciences, Tabriz, Iran & School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
4- Department of Biology, Faculty of Science, Shahid Chamran University, Ahvaz, Iran
5- Department of Microbiology, Ahar Branch, Islamic Azad University, Ahar, Iran

Abstract: (639 Views)

Background and Aim: Escherichia coli (E. coli) O157:H7 is the primary cause of food poisoning and urinary tract infection via Shiga toxin. The main transcription factor for Shiga toxin is Hfq gene. It is reported that probiotics can reduce the production of toxin substances in pathogenic bacteria. This study was aimed to investigate the influence of Bifidobacterium longum (B. longum) on the expression level of the Hfq gene as the main virulence factor in E. coli O157:H7.
Materials and Methods: B. longum was cultured in MRS (De Man, Rogosa, and Sharpe) medium. Different concentrations of B. longum was co-cultured in Mueller-Hinton Agar (MHA) along with E. coli in different time points. Total RNA was extracted followed by synthesizing cDNA. The Hfq gene expression was evaluated by SYBR Green real-time PCR. The 16S ribosomal RNA was used as an internal control for data normalization.
Results: The results showed reduction in E. coli growth at different co-culture time points with B. longum . Molecular analysis demonstrated that B. longum has the ability to significantly reduce the expression of Hfq virulence gene (P=0.0023).
Conclusion: Probiotic bacteria inhibit the growth of pathogenic bacteria by producing bacteriocins and reducing the expression level of the virulence genes. They can also play role in inhibiting intoxications and infections caused by E. coli.

Keywords: Bifidobacterium longum, E. coli, Hfq gene, Probiotics

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Type of Study: Original Research Article | Subject: Molecular Microbiology
Received: 2024/08/11 | Accepted: 2024/11/20 | ePublished: 2024/11/30

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