In vitro Evaluation of Yazd Honey Effect on Leishmania major [MRHO/IR/75/ER] Promastigotes

Fattahi Bafghi, Ali; Eslami, Gilda; Emtiazi, Hamideh; Mozayan, Mohammad Reza; Zarei, Elmira; Bagheri, Parisa; Rezaee, Elham

doi:10.30699/ijmm.18.1.49

year 18, Issue 1 (January - February 2024) Iran J Med Microbiol 2024, 18(1): 49-56 | Back to browse issues page

‎ 10.30699/ijmm.18.1.49

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Fattahi Bafghi A, Eslami G, Emtiazi H, Mozayan M R, Zarei E, Bagheri P et al . In vitro Evaluation of Yazd Honey Effect on Leishmania major [MRHO/IR/75/ER] Promastigotes. Iran J Med Microbiol 2024; 18 (1) :49-56
URL: http://ijmm.ir/article-1-2203-en.html

In vitro Evaluation of Yazd Honey Effect on Leishmania major [MRHO/IR/75/ER] Promastigotes

Ali Fattahi Bafghi¹

, Gilda Eslami¹

, Hamideh Emtiazi²

, Mohammad Reza Mozayan³

, Elmira Zarei⁴

, Parisa Bagheri⁵

, Elham Rezaee⁶

1- Department of Parasitology and Mycology, Infectious Diseases Research Center, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2- Faculty of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
3- Department of General Courses, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
4- Department of Hematology and Blood Banking, School of Allied, Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran
5- Department of Hematology and Blood Banking, Paramedical School, Gerash University of Medical Sciences, Gerash, Iran
6- Department of Medical Parasitology and Mycology, Faculty of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran , Rezaee.para92@gmail.com

Abstract: (976 Views)

Background and Aim: Leishmaniasis is a parasitic illness carried by the bite of some species of sand flies. The cause of the disease is protozoan parasites of the genus leishmania. This study investigated the in vitro evaluation of Yazd honey effect on Leishmania major [MRHO/IR/75/ER] promastigotes in stationary and logarithmic phases.
Materials and Methods: Multifloral honey obtained by Yazd's experienced beekeepers (Yazd, Iran) in the 2016–2017 harvest season was included in the study. The L. major strain, cataloged as [MRHO/IR/75/ER], was cultured in BALB/c mice. We extracted amastigotes from the spleens of these mice, which we then converted into promastigotes within the Novy-MacNeal-Nicolle (NNN) medium. The L. major strain was then carefully supplied at various times, and the parasite populations within the specified culture were monitored and quantified using both slide microscopy and enzyme-linked immunosorbent assay (ELISA) techniques. We started with a consistent amount of parasites and placed them into vials with screw-caps filled with 5 mL of a liquid medium. This medium was infused with honey at various levels, including 6.25, 12.5, 25, and 50 micrograms per milliliter. These vials were then incubated for several durations: starting from zero and continuing at 6, 12, 24, 48, 72, and 96 hours.
Results: The results of the counts taken were measured against those of the control group and showed that parasite load reduced with increasing Yazd honey concentrations (6.25 to 50 µg/mL) and contact duration (6-96 hours).
Conclusion: The findings revealed that honey has the capacity to curb the growth and survival of parasites, positioning it as a potential subject for research in both animals and humans.

Keywords: Honey of Yazd, In vitro, Leishmania major, Viability

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Type of Study: Original Research Article | Subject: Medical Parasitology
Received: 2023/11/27 | Accepted: 2024/02/17 | ePublished: 2024/03/18

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