year 18, Issue 1 (January - February 2024)                   Iran J Med Microbiol 2024, 18(1): 49-56 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Fattahi Bafghi A, Eslami G, Emtiazi H, Mozayan M R, Zarei E, Bagheri P et al . In vitro Evaluation of Yazd Honey Effect on Leishmania major [MRHO/IR/75/ER] Promastigotes. Iran J Med Microbiol 2024; 18 (1) :49-56
1- Department of Parasitology and Mycology, Infectious Diseases Research Center, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2- Faculty of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
3- Department of General Courses, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
4- Department of Hematology and Blood Banking, School of Allied, Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran
5- Department of Hematology and Blood Banking, Paramedical School, Gerash University of Medical Sciences, Gerash, Iran
6- Department of Medical Parasitology and Mycology, Faculty of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran ,
Abstract:   (231 Views)

Background and Aim: Leishmaniasis is a parasitic illness carried by the bite of some species of sand flies. The cause of the disease is protozoan parasites of the genus leishmania. This study investigated the in vitro evaluation of Yazd honey effect on Leishmania major [MRHO/IR/75/ER] promastigotes in stationary and logarithmic phases.
Materials and Methods: Multifloral honey obtained by Yazd's experienced beekeepers (Yazd, Iran) in the 2016–2017 harvest season was included in the study. The L. major strain, cataloged as [MRHO/IR/75/ER], was cultured in BALB/c mice. We extracted amastigotes from the spleens of these mice, which we then converted into promastigotes within the Novy-MacNeal-Nicolle (NNN) medium. The L. major strain was then carefully supplied at various times, and the parasite populations within the specified culture were monitored and quantified using both slide microscopy and enzyme-linked immunosorbent assay (ELISA) techniques. We started with a consistent amount of parasites and placed them into vials with screw-caps filled with 5 mL of a liquid medium. This medium was infused with honey at various levels, including 6.25, 12.5, 25, and 50 micrograms per milliliter. These vials were then incubated for several durations: starting from zero and continuing at 6, 12, 24, 48, 72, and 96 hours.
Results: The results of the counts taken were measured against those of the control group and showed that parasite load reduced with increasing Yazd honey concentrations (6.25 to 50 µg/mL) and contact duration (6-96 hours).
Conclusion: The findings revealed that honey has the capacity to curb the growth and survival of parasites, positioning it as a potential subject for research in both animals and humans.

Full-Text [PDF 619 kb]   (44 Downloads)    
Type of Study: Original Research Article | Subject: Medical Parasitology
Received: 2023/11/27 | Accepted: 2024/02/17 | ePublished: 2024/03/18

1. Fattahi Bafghi A, Rezaee E, Ahmadi S, Gholamrezaei M, Firouzeh N. Review of Molecular Approaches in Leishmaniasis Vaccines: Implications and Restrictions. Int J Infect. 2022;9(2):e121894. [DOI:10.5812/iji-121894]
2. Ramezankhani R, Sajjadi N, Nezakati esmaeilzadeh R, Jozi SA, Shirzadi MR. Climate and environmental factors affecting the incidence of cutaneous leishmaniasis in Isfahan, Iran. Environ Sci Pollut Res Int. 2018;25:11516-26. [DOI:10.1007/s11356-018-1340-8] [PMID]
3. Golpayegani AA, Moslem AR, Akhavan AA, Zeydabadi A, Mahvi AH, Allah-Abadi A. Modeling of Environmental Factors Affecting the Prevalence of Zoonotic and Anthroponotic Cutaneous, and Zoonotic Visceral Leishmaniasis in Foci of Iran: a Remote Sensing and GIS Based Study. J Arthropod Borne Dis. 2018;12(1):41-66.
4. Malek MS, Robi IH, Islam MS, Kabir MA, Uddin MZ, Sumon SM, et aI. Clinical and Hematological Features of Visceral Leishmaniasis at Mymensingh Medical College Hospital. Mymensingh Med J. 2020;29(4):879-86.
5. Volpedo G, Pacheco-Fernandez T, Holcomb EA, Cipriano N, Cox B, Satoskar AR. Mechanisms of immunopathogenesis in cutaneous leishmaniasis and post kala-azar dermal leishmaniasis (PKDL). Front Cell Infect Microbiol. 2021;11:685296. [DOI:10.3389/fcimb.2021.685296] [PMID] [PMCID]
6. Mohammadbeigi A, Khazaei S, Heidari H, Asgarian A, Arsangjang S, Saghafipour A, et al. An investigation of the effects of environmental and ecologic factors on cutaneous leishmaniasis in the old world: a systematic review study. Rev Environ Health. 2021;36(1):117-28. [DOI:10.1515/reveh-2020-0066] [PMID]
7. de Souza RAF, Andreoli RV, Kayano MT, Carvalho AL. American cutaneous leishmaniasis cases in the metropolitan region of Manaus, Brazil: association with climate variables over time. Geospat Health. 2015;10(1):314. [DOI:10.4081/gh.2015.314] [PMID]
8. Seid A, Gadisa E, Tsegaw T, Abera A, Teshome A, Mulugeta A, et al. Risk map for cutaneous leishmaniasis in Ethiopia based on environmental factors as revealed by geographical information systems and statistics. Geospat Health. 2014;8(2):377-87. [DOI:10.4081/gh.2014.27] [PMID]
9. Hartley MA, Drexler S, Ronet C, Beverley SM, Fasel N. The immunological, environmental, and phylogenetic perpetrators of metastatic leishmaniasis. Trends Parasitol. 2014;30(8):412-22. [DOI:10.1016/] [PMID] [PMCID]
10. Den Boer M, Argaw D, Jannin J, Alvar J. Leishmaniasis impact and treatment access. Clin Microbiol Infect. 2011;17(10):1471-7. [DOI:10.1111/j.1469-0691.2011.03635.x] [PMID]
11. Alvar J, Yactayo S, Bern C. Leishmaniasis and poverty. Trends Parasitol. 2006;22(12):552-7. [DOI:10.1016/] [PMID]
12. MMusa YM, Haruna AK, Ilyas M, Yaro AH, Ahmadu AA, Usman H. Phytochemical, analgesic and anti-inflammatory effects of the ethylacetate extract of the leaves of Pseudocedrella kotschyii. Afr J Tradit Complement Altern Med. 2008;5(1):92-6. [DOI:10.4314/ajtcam.v5i1.31261] [PMID] [PMCID]
13. Pospíšil Jr, Konrádová D, Strnad M. Antileishmanial Activity of Lignans, Neolignans, and Other Plant Phenols. Prog Chem Org Nat Prod. 2021;115:115-76. [DOI:10.1007/978-3-030-64853-4_3] [DOI:10.1007/978-3-030-64853-4_5] [PMID]
14. Parvandi M, Rezadoost H, Farzaneh M. Introducing Alternaria tenuissima SBUp1, as an endophytic fungus of Ferula assa‐foetida from Iran, which is a rich source of rosmarinic acid. Lett Appl Microbiol. 2021;73(5):569-78. [DOI:10.1111/lam.13542] [PMID]
15. Seraglio SK, Schulz M, Brugnerotto P, Silva B, Gonzaga LV, Fett R, et al. Quality, composition and health-protective properties of citrus honey: A review. Food Res Int. 2021;143:110268. [DOI:10.1016/j.foodres.2021.110268] [PMID]
16. Mustafa G, Iqbal A, Javid A, Hussain A, Bukhari SM, Ali W, et al. Variations in nutritional profile of honey produced by various species of genus Apis. Braz J Biol. 2021;83:e246651. [DOI:10.1590/1519-6984.246651] [PMID]
17. Ding Y, Xu M, Lu Q, Wei P, Tan J, Liu R. Combination of honey with metformin enhances glucose metabolism and ameliorates hepatic and nephritic dysfunction in STZ-induced diabetic mice. Food Func. 2019;10(11):7576-87. [DOI:10.1039/C9FO01575B] [PMID]
18. Akimov MY, Bessonov VV, Kodentsova VM, Eller KI, Vrzhesinskaya OA, Beketova NA, et al. Biological value of fruits and berries of Russian production. Prob Nutr. 2020;89(4):220-32.
19. Ng WJ, Sit NW, Ooi PA, Ee KY, Lim TM. The antibacterial potential of honeydew honey produced by stingless bee (Heterotrigona itama) against antibiotic resistant bacteria. Antibiotics. 2020;9(12):871. [DOI:10.3390/antibiotics9120871] [PMID] [PMCID]
20. Bassam Z, Zohra BI, Saada AA. The effects of honey on Leishmania parasites: an in vitro study. Trop Doctor. 1997;27(1_suppl):36-8. [DOI:10.1177/00494755970270S112]
21. Murray HW, Berman JD, Davies CR, Saravia NG. Advances in leishmaniasis. The Lancet. 2005;366(9496):1561-77. [DOI:10.1016/S0140-6736(05)67629-5] [PMID]
22. Alzahrani HA, Alsabehi R, Boukraâ L, Abde-llah F, Bellik Y, Bakhotmah BA. Antibacterial and antioxidant potency of floral honeys from different botanical and geographical origins. Molecules. 2012;17(9):10540-9. [DOI:10.3390/molecules170910540] [PMID] [PMCID]
23. Endo EH, Makimori RY, Companhoni MV, Ueda-Nakamura T, Nakamura CV, Dias Filho BP. Ketoconazole-loaded poly-(lactic acid) nanoparticles: Characterization and improvement of antifungal efficacy in vitro against Candida and dermatophytes. J Mycol Med. 2020;30(3):101003. [DOI:10.1016/j.mycmed.2020.101003] [PMID]
24. Ghapanchi J, Moattari A, Tadbir AA, Talatof Z. Sara Pour shahidi, Hooman Ebrahimi. The in vitro anti-viral activity of Honey on Type 1 Herpes simplex virus. Aust J Basic Appl Sci. 2011;5(12):849-52.
25. Shendge AK, Chaudhuri D, Mandal N. The natural flavones, acacetin and apigenin, induce Cdk-Cyclin mediated G2/M phase arrest and trigger ROS-mediated apoptosis in glioblastoma cells. Mol Biol Rep. 2021;48(1):539-49. [DOI:10.1007/s11033-020-06087-x] [PMID]
26. Parvizi MM, Zare F, Handjani F, Nimrouzi M, Zarshenas MM. Overview of herbal and traditional remedies in the treatment of cutaneous leishmaniasis based on Traditional Persian Medicine. Dermatol Ther. 2020;33(4):e13566. [DOI:10.1111/dth.13566]
27. Aksoy T, Sivcan E, Doğan F, Çetin S, Yar TM. Investigation of anti-leishmanial effects of bee products (honey, propolis) on Leishmania tropica promastigotes. Mikrobiyoloji Bulteni. 2020;54(3):479-89. [DOI:10.5578/mb.69632] [PMID]
28. Gholizadeh H, Ghaffarifar F, Dalimi A, Dayer MS. In vitro and in vivo effects of natural honey on Leishmania major. Ann Parasitol. 2022;68(1):71-6.
29. Sinha S, Sehgal A, Ray S, Sehgal R. Benefits of Manuka Honey in the Management of Infectious Diseases: Recent Advances and Prospects. Mini Rev Med Chem. 2023;23(20):1928-41. [DOI:10.2174/1389557523666230605120717] [PMID]
30. Sherafati J, Dayer MS, Ghaffarifar F, Akbarzadeh K, Pirestani M. Evaluating leishmanicidal effects of Lucilia sericata products in combination with Apis mellifera honey using an in vitro model. Plos One. 2023;18(8):e0283355. [DOI:10.1371/journal.pone.0283355] [PMID] [PMCID]
31. Almasaudi S. The antibacterial activities of honey. Saudi J Biol Sci. 2021;28(4):2188-96. [DOI:10.1016/j.sjbs.2020.10.017] [PMID] [PMCID]
32. Fernandes L, Ribeiro H, Oliveira A, Silva AS, Freitas A, Henriques M, et al. Portuguese honeys as antimicrobial agents against Candida species. J Tradit Complement Med. 2021;11(2):130-6. [DOI:10.1016/j.jtcme.2020.02.007] [PMID] [PMCID]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Iranian Journal of Medical Microbiology

Designed & Developed by : Yektaweb | Publisher: Farname Inc