year 17, Issue 4 (July - August 2023)                   Iran J Med Microbiol 2023, 17(4): 432-440 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mahmood Mahdy D, Nazar Mahmood N, Shawkat Ali W. Antagonistic Activity of Bacteriocin-Producing Lactobacillus Local Isolates Against Select Pathogenic Bacterial Strains. Iran J Med Microbiol 2023; 17 (4) :432-440
1- Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq ,
2- Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq
3- Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq
Abstract:   (390 Views)

Background and Aim: Lactobacilli are known for their probiotic activities and potential to combat pathogens. This study aimed to investigate the antimicrobial activity of Lactobacillus strains isolated from various sources against pathogenic bacteria, with the objective of identifying potential bacteriocin producers.
Materials and Methods: A total of 140 samples were collected from different sources. Samples were cultured on selective media such as MRS and SL agar for isolation of Lactobacillus spp under anaerobic conditions. The suspected bacteria appeared Gram positive, diploid bacilli or coccobacilli. The isolates were subjected to various biochemical tests such as catalase, oxidase, also hydrolysis test was done by growing on MRS containing 1% calcium carbonate. The antimicrobial activity of bacteriocin was screened by using three method including (Agar-plug diffusion method, Filter paper disc method and agar-wells diffusion method). Molecular identification of the best bacteriocin producer was achieved using 16S rRNA sequencing.
Results: The study found that L. helveticus DF isolated from feces exhibited the highest bacteriocin production efficiency. Concentrated bacteriocin from L. helveticus DF demonstrated potent antibacterial activity against multi-drug resistant bacteria, with 80 AU/ml against Pseudomonas aeruginosa and 40 AU/ml against Enterococcus faecalis.
Conclusion: The study highlights the potential of Lactobacillus bacteriocins as effective antibacterial agents, offering a promising alternative to combat antibiotic resistance. Further research is warranted to elucidate the mechanisms and assess the safety and efficacy of bacteriocins for their use as bio-therapeutic agents.

Full-Text [PDF 878 kb]   (111 Downloads) |   |   Full-Text (HTML)  (59 Views)  
Type of Study: Original Research Article | Subject: Medical Bacteriology
Received: 2023/06/21 | Accepted: 2023/09/1 | ePublished: 2023/09/27

1. Kumar A, Schweizer HP. Bacterial resistance to antibiotics: active efflux and reduced uptake. Adv Drug Deliv Rev. 2005;57(10):1486-513. [DOI:10.1016/j.addr.2005.04.004] [PMID]
2. Hosien B, Belhaj H, Atia A. Characteristics of antibiotic-resistant bacteria in Libya based on different source of infections. Libyan Int Med Univ J. 2022;7(02):039-044. [DOI:10.1055/s-0042-1759621]
3. 3. Ramzah NHHL, Yenn TW, Lee WH, Loo CY, Tan WN, Ring LC. Antimicrobial Peptides, An Alternative Antimicrobial Agent Against Multi-drug-Resistant Microbes: Source, Application, and Potential. In: Ismail A, Nur Zulkipli F, Husin HS, Öchsner A. (eds) Advancements in Materials Science and Technology Led by Women. Advanced Structured Materials, vol 165. Springer, Cham. [DOI:10.1007/978-3-031-21959-7_17]
4. Joni FH. Isolation and diagnosis of bacteria causing urinary tract infection in pregnant and non-pregnant females with diabetes mellitus type2. Al-Mustansiriyah J Sci. 2018;29(4):23-6. [DOI:10.23851/mjs.v29i4.465]
5. Stoyanova LG, Ustyugova EA, Netrusov AI. Antibacterial metabolites of lactic acid bacteria: their diversity and properties. Appl Biochem Microbiol. 2012;48:229-43. [DOI:10.1134/S0003683812030143]
6. Sharifi Yazdi MK, Davoodabadi A, Khesht Zarin HR, Tajabadi Ebrahimi M, Soltan Dallal MM. Characterisation and probiotic potential of lactic acid bacteria isolated from Iranian traditional yogurts. Ital J Anim Sci. 2017;16(2):185-8. [DOI:10.1080/1828051X.2016.1222888]
7. Zacharof MP, Lovitt R. Bacteriocins produced by lactic acid bacteria a review article. APCBEE Procedia. 2012;2:50-6. [DOI:10.1016/j.apcbee.2012.06.010]
8. Gutiérrez-Cortés C, Suarez H, Buitrago G, Nero LA, Todorov SD. Characterization of bacteriocins produced by strains of Pediococcus pentosaceus isolated from Minas cheese. Ann Microbiol. 2018; 68(6):383-98.‏ [DOI:10.1007/s13213-018-1345-z]
9. Hassan MU, Nayab H, Rehman TU, Williamson MP, Haq K U, Shafi N, et al. Characterisation of bacteriocins produced by Lactobacillus spp. isolated from the traditional Pakistani yoghurt and their antimicrobial activity against common foodborne pathogens. Biomed Res Int. 2020;2020.‏ [DOI:10.1155/2020/8281623] [PMID] [PMCID]
10. Manoharan M, Balasubramaniam TS. An Extensive Review on Production, Purification, and Bioactive Application of Different Classes of Bacteriocin. J Trop Biotechnol Biodivers. 2022;7(3):72735.‏ [DOI:10.22146/jtbb.72735]
11. Corr SC, Li Y, Riedel CU, O'Toole PW, Hill C, Gahan CG. Bacteriocin production as a mechanism for the anti-infective activity of Lactobacillus salivarius UCC118. Proc Natl Acad Sci. 2007;104(18):7617-21. [DOI:10.1073/pnas.0700440104] [PMID] [PMCID]
12. Thirabunyanon M, Thongwittaya N. Protection activity of a novel probiotic strain of Bacillus subtilis against Salmonella Enteritidis infection. Res vet Sci. 2012;93(1):74-81.‏ [DOI:10.1016/j.rvsc.2011.08.008] [PMID]
13. Karami S, Roayaei M, Hamzavi H, Bahmani M, Hassanzad-Azar H, Leila M, et al. Isolation and identification of probiotic Lactobacillus from local dairy and evaluating their antagonistic effect on pathogens. Int J Pharm Investig. 2017;7(3):137-41. [DOI:10.4103/jphi.JPHI_8_17] [PMID] [PMCID]
14. Mirzaei H, Barzgari A. Isolation and molecular study of potentially probiotic lactobacilli in traditional white cheese of Tabriz in Iran. Ann Biological Res 2012;3: 2213-2216.
15. Atassi F, Brassart D, Grob P, Graf F, Servin AL. Lactobacillus strains isolated from the vaginal microbiota of healthy women inhibit Prevotella bivia and Gardnerella vaginalis in coculture and cell culture. FEMS Immunol Med Microbiol. 2006; 48(3):424-32. [DOI:10.1111/j.1574-695X.2006.00162.x] [PMID]
16. Loy A, Lehner A, Lee N, Adamczyk J, Meier H, Ernst J, et al. Oligonucleotide microarray for 16S rRNA gene-based detection of all recognized lineages of sulfate-reducing prokaryotes in the environment. Appl Environ Microbiol. 2002;68(10):5064-81. [DOI:10.1128/AEM.68.10.5064-5081.2002] [PMID] [PMCID]
17. Rahman M, Nahidul Islam MD, Nurul Islam M, Shahnoor HM. Isolation and Identification of Oral Bacteria and Characterization for Bacteriocin Production and Antimicrobial Sensitivity. J Pharm Sci Dhaka Univ. 2015;14(1):103-9. [DOI:10.3329/dujps.v14i1.23742]
18. Dyaee NA, Luti KJK. Classical and Statistical Optimization by Response Surface Methodology For Enhancing Biomass and Bacteriocin Production by Lactobacillus Plantarum. Iraqi J Sci. 2019;60(3):494-508.
19. Biyari S, Fozouni L. The inhibitory effect of probiotic bacteria against drug-resistant Candida species isolated from the oral cavity of the elderly. Shiraz E-Med J. 2018;19(6):2-7. [DOI:10.5812/semj.62026]
20. Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: a review. J Pharm Anal. 2016;6(2):71-9. [DOI:10.1016/j.jpha.2015.11.005] [PMID] [PMCID]
21. Hussain MA. Antibacterial effect of probiotic lactic acid bacteria on some pathogens. J Genes Environ Resour Conserv. 2022;10(1):1-6.
22. Dai J, Fang L, Zhang M, Deng H, Cheng X, Yao M, et L. Isolation and identification of new source of bacteriocin-producing Lactobacillus plantarum C010 and growth kinetics of its batch fermentation. World J Microbiol Biotechnol. 2022;38(4):67. [DOI:10.1007/s11274-022-03244-1] [PMID]
23. Antonio MAD, Hawes SE, Hillier SL. The identification of vaginal Lactobacillus species and the demographic and microbiologic characteristics of women colonized by these species. J Infect Dis. 1999;180(6):1950-6. [DOI:10.1086/315109] [PMID]
24. Ibrahim SA, Ayivi R D, Zimmerman T, Siddiqui S A, Altemimi AB, Fidan H. et al. Lactic acid bacteria as antimicrobial agents: Food safety and microbial food spoilage prevention. Foods. 2021;10(12):3131.‏ [DOI:10.3390/foods10123131] [PMID] [PMCID]
25. Gu Q. Bacteriocinogenic Lactic Acid Bacteria and Antibacterial Mechanisms. In: Bacteriocins. Singapore: Springer Nature Singapore. 2023. p. 39-61.‏ [DOI:10.1007/978-981-99-2661-9_3]
26. Dubernet S, Desmasures N, Guéguen M. A PCRbased method for identification of lactobacilli at the genus level. FEMS Microbiol Lett. 2002; 214(2):271-5. [DOI:10.1111/j.1574-6968.2002.tb11358.x] [PMID]
27. Tagg JR, Read RSD, McGiven AR. Bacteriocine production by group A streptococci. Pathology. 1971;3(4):277-8.‏ [DOI:10.3109/00313027109073746]
28. Rogers AM, Montville TJ. Improved agar diffusion assay for nisin quantification. Food Biotechnol. 1991;5(2):161-8.‏ [DOI:10.1080/08905439109549799]
29. Champiri ID, Bamzadeh Z, Rahimi E, Rouhi L. Isolation and identification of Lactobacillus brevis from local cheese of bazoft and evaluation of antimicrobial activity against some pathogenic microorganisms. Iran J Med Microbiol. 2022;16(1):17-34.‏ [DOI:10.30699/ijmm.16.1.17]
30. Mahmoud Farhan S, Mahmoud Abd El-Baky R, Abdalla M, Osama El-Gendy A, Farag Azmy A. Efficacy of Amikacin and Imipenem Against Multi-Drug Resistant Gram-Negative Bacteria Isolated from Wound Infections, Egypt. Iran J Med Microbiol. 2023;17(2):218-29.‏ [DOI:10.30699/ijmm.17.2.218]
31. Ghorani M. Antiviral Effects of Probiotic Metabolites. Iran J Med Microbiol. 2022;16(2):83-97.‏ [DOI:10.30699/ijmm.16.2.83]
32. Ghorbani Z, Koupaei M, Saderi H, Amin Marashi SM, DehghanZadeh Z, & Owlia P. Inhibitory Effect of Supernatant and Lysate of Saccharomyces cerevisiae on Expression of exoA Gene of Pseudomonas aeruginosa. Iran J Med Microbiol. 2023;17(3):288-93. [DOI:10.30699/ijmm.17.3.288]
33. Altalhi AA. Antilisterial activity of plantaricin UG1 during manufacture of Zabady and Kareesh cheese: Two Arabian dairy products. Int J Biomed Sci. 2008;4(4):319.
34. Aziz RA, Salman, JAS, Hamel AH. Study of the Synergistic effect between purified and produced bacteriocin from bacteria Lactococcus lactis ssp. lactis Against Species of Pathogenic bacteria. J Coll Basic Educ. 2020;26:107.
35. Jamalifar H, Rahimi H, Samadi N, Shaverdi A, Sharifian Z, Hosseini F, et al. Antimicrobial activity of different Lactobacillus species against multi-drug resistant clinical isolates of Pseudomonas aeruginosa. Iran J Microbiol. 2011;3(1):21-5.
36. Zohreh-Mirbagheri S, Meshkat Z, Naderinasab M, Rostami S, Sadat-Nabavinia M, Rahmati M. Study on imipenem resistance and prevalence of blaVIM1 and blaVIM2 metallo-beta lactamases among clinical isolates of Pseudomonas aeruginosa from Mashhad, Northeast of Iran. Iran J Microbiol. 2015;7(2):72-8.

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2023 CC BY-NC 4.0 | Iranian Journal of Medical Microbiology

Designed & Developed by : Yektaweb | Publisher: Farname Inc