Anticancer and Antibacterial Effects of Blueberry Fruit (Vaccinium corymbosum L.) in Three Developmental Stages

Amin Salehi, Mahsa; Chehregani Rad, Abdolkarim; Afshar, Saeid

doi:10.30699/ijmm.17.5.613

year 17, Issue 5 (September - October 2023) Iran J Med Microbiol 2023, 17(5): 613-619 | Back to browse issues page

‎ 10.30699/ijmm.17.5.613

Mendeley

Zotero

RefWorks

Amin Salehi M, Chehregani Rad A, Afshar S. Anticancer and Antibacterial Effects of Blueberry Fruit (Vaccinium corymbosum L.) in Three Developmental Stages. Iran J Med Microbiol 2023; 17 (5) :613-619
URL: http://ijmm.ir/article-1-2142-en.html

Anticancer and Antibacterial Effects of Blueberry Fruit (Vaccinium corymbosum L.) in Three Developmental Stages

Mahsa Amin Salehi¹

, Abdolkarim Chehregani Rad²

, Saeid Afshar³

1- Department of Plant Science, Faculty of Sciences, Bu-Ali Sina University, Hamedan, Iran
2- Department of Plant Science, Faculty of Sciences, Bu-Ali Sina University, Hamedan, Iran , a.chehregani@gmail.com
3- Cancer Research Center, Hamadan University of Medical Sciences, Hamadan, Iran

Abstract: (1335 Views)

Background and Aim: Blueberries, scientifically known as Vaccinium corymbosum L., contain various secondary metabolites such as flavonoids and phenolic acids. These compounds are recognized for their potential in preventing cancer and antimicrobial properties.
Materials and Methods: The extract's antibacterial activity was assessed using the time-killing assay, while its anticancer activity was evaluated using the MTT assay.
Results & Conclusion: The findings from the antibacterial tests revealed that the fruit extracts exhibited minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) at concentrations of 3.5-4.2 mg/mL and 5.6-5.9 mg/mL, respectively. Furthermore, the MTT assay demonstrated that the blueberry extract displayed cytotoxic effects on cancer cell lines (HeLa and MCF7), while it showed no significant cytotoxicity on normal cells (L929). Treatment with the unripe blueberry fruit extract significantly decreased the viability of cancer cells compared to the other extracts. Specifically, the highest level of cellular toxicity among all extracts was observed in the HeLa cancer cell line at a concentration of 1.5 mg/L after 72 hours (more than 90%). Based on these findings, it can be concluded that blueberry fruit extract possesses enhanced antibacterial and anticancer properties. Moreover, this study indicated that the early stages of fruit growth exhibit greater cytotoxic effects against cancer and bacterial cells. These results significantly affect the food, pharmaceutical, and plant biotechnology industries.

Keywords: HCMV, Human Cytomegalovirus, Iran, Molecular Prevalence, Prostate Neoplasm, Prostate Cancer

Full-Text [PDF 590 kb] (463 Downloads) | | Full-Text (HTML) (223 Views)

Type of Study: Brief Original Article | Subject: Antimicrobial Substances
Received: 2023/07/26 | Accepted: 2023/10/26 | ePublished: 2023/11/29

References

1. Wu Y, Yang H, Yang H, Zhang C, Lyu L, Li W, et al. A physiological and metabolomic analysis reveals the effect of shading intensity on blueberry fruit quality. Food Chem X. 2022;15:100367. [DOI:10.1016/j.fochx.2022.100367] [PMID] [PMCID]

2. Shahniani A, Bamzadeh Z, Mahmoudnia F, Rouhi L. In vitro Apoptosis Induction in Prostate Cancer Cells (PC-3) using Bacillus licheniformis Supernatant. Iran J Med Microbiol. 2020;14(6):618-42. [DOI:10.30699/ijmm.14.6.618]

3. Kiddane AT, Kang M-J, Ho TC, Getachew AT, Patil MP, Chun B-S, et al. Anticancer and Apoptotic Activity in Cervical Adenocarcinoma HeLa Using Crude Extract of Ganoderma applanatum. Curr Issues Mol Biol. 2022;44(3):1012-26. [DOI:10.3390/cimb44030067] [PMID] [PMCID]

4. Shameli Rajiri M, Aminsalehi M, Shahbandeh M, Maleki A, Jonoubi P, Rad AC. Anticancer and therapeutic potential of Delonix regia extract and silver nanoparticles (AgNPs) against pancreatic (Panc-1) and breast (MCF-7) cancer cell. Toxicol Environ Health Sci. 2021;13(1):45-56. [DOI:10.1007/s13530-020-00067-1]

5. Ștefănescu R, Laczkó-Zöld E, Ősz B-E, Vari C-E. An Updated Systematic Review of Vaccinium myrtillus Leaves: Phytochemistry and Pharmacology. Pharmaceutics. 2022;15(1):16. [DOI:10.3390/pharmaceutics15010016] [PMID] [PMCID]

6. Moghadam S, Moradi Bidhendi S, Khaki P. Molecular Identification of Salmonella Strains Isolated from Livestock in Alborz Province and Their Serotyping. Iran J Med Microbiol. 2022;16(4):305-11. [DOI:10.30699/ijmm.16.4.305]

7. Bouyahya A, Omari NE, El Hachlafi N, Jemly ME, Hakkour M, Balahbib A, et al. Chemical Compounds of Berry-Derived Polyphenols and Their Effects on Gut Microbiota, Inflammation, and Cancer. Molecules. 2022;27(10). [DOI:10.3390/molecules27103286] [PMID] [PMCID]

8. Cerezo AB, Cătunescu GM, González MM, Hornedo-Ortega R, Pop CR, Rusu CC, et al. Anthocyanins in Blueberries Grown in Hot Climate Exert Strong Antioxidant Activity and May Be Effective against Urinary Tract Bacteria. Antioxidants. 2020;9(6). [DOI:10.3390/antiox9060478] [PMID] [PMCID]

9. Singh N, Yadav SS. A review on health benefits of phenolics derived from dietary spices. Curr Res Nutr Food Sci. 2022;5:1508-23. [DOI:10.1016/j.crfs.2022.09.009] [PMID] [PMCID]

10. Appiah T, Boakye YD, Agyare C. Antimicrobial Activities and Time-Kill Kinetics of Extracts of Selected Ghanaian Mushrooms. Evid Based Complementary Altern Med. 2017;2017:4534350. [DOI:10.1155/2017/4534350] [PMID] [PMCID]

11. Hasan Abdali M, Afshar S, Sedighi Pashaki A, Dastan D, Gholami MH, Mahmoudi R, et al. Investigating the effect of radiosensitizer for Ursolic Acid and Kamolonol Acetate ‌ on HCT-116 cell line. Bioorg Med Chem. 2020;28(1):115152. [DOI:10.1016/j.bmc.2019.115152] [PMID]

12. Baenas N, Ruales J, Moreno DA, Barrio DA, Stinco CM, Martínez-Cifuentes G, et al. Characterization of Andean Blueberry in Bioactive Compounds, Evaluation of Biological Properties, and In Vitro Bioaccessibility. Foods. 2020;9(10). [DOI:10.3390/foods9101483] [PMID] [PMCID]

13. Guevara-Terán M, Padilla-Arias K, Beltrán-Novoa A, González-Paramás AM, Giampieri F, Battino M, et al. Influence of Altitudes and Development Stages on the Chemical Composition, Antioxidant, and Antimicrobial Capacity of the Wild Andean Blueberry (Vaccinium floribundum Kunth). Molecules. 2022;27(21). [DOI:10.3390/molecules27217525] [PMID] [PMCID]

14. Routray W, Orsat V. Blueberries and Their Anthocyanins: Factors Affecting Biosynthesis and Properties. Compr Rev Food Sci Food Saf. 2011;10(6):303-20. [DOI:10.1111/j.1541-4337.2011.00164.x]

15. Pap N, Fidelis M, Azevedo L, do Carmo MAV, Wang D, Mocan A, et al. Berry polyphenols and human health: evidence of antioxidant, anti-inflammatory, microbiota modulation, and cell-protecting effects. Curr Opin Food Sci. 2021;42:167-86. [DOI:10.1016/j.cofs.2021.06.003]

16. Kiernozek E, Maslak P, Kozlowska E, Jarzyna I, Średnicka-Tober D, Hallmann E, et al. Biological Activity of Extracts from Differently Produced Blueberry Fruits in Inhibiting Proliferation and Inducing Apoptosis of HT-29 Cells. Foods. 2022;11(19). [DOI:10.3390/foods11193011] [PMID] [PMCID]

17. Hwang H, Kim Y-J, Shin Y. Influence of ripening stage and cultivar on physicochemical properties, sugar and organic acid profiles, and antioxidant compositions of strawberries. Food Sci Biotechnol. 2019;28(6):1659-67. [DOI:10.1007/s10068-019-00610-y] [PMID] [PMCID]

18. Yang H, Kim Y-J, Shin Y. Influence of Ripening Stage and Cultivar on Physicochemical Properties and Antioxidant Compositions of Aronia Grown in South Korea. Foods. 2019;8(12). [DOI:10.3390/foods8120598] [PMID] [PMCID]

19. Shin Y, Ryu J-A, Liu RH, Nock JF, Watkins CB. Harvest maturity, storage temperature and relative humidity affect fruit quality, antioxidant contents and activity, and inhibition of cell proliferation of strawberry fruit. Postharvest Biol Technol. 2008;49(2):201-9. [DOI:10.1016/j.postharvbio.2008.02.008]