year 17, Issue 5 (September - October 2023)
Iran J Med Microbiol 2023, 17(5): 571-584 |
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Fathi M, Barzegari E, Lotfollahi L, Jafari R, Nomanpour B, Rasekhian M. Outer membrane Proteins-focused Pseudomonas aeruginosa Vaccine Designed using Reverse Vaccinology. Iran J Med Microbiol 2023; 17 (5) :571-584
URL: http://ijmm.ir/article-1-2125-en.html
URL: http://ijmm.ir/article-1-2125-en.html
Masoumeh Fathi1 
, Ebrahim Barzegari2 , Lida Lotfollahi3 , Reza Jafari4 , Bizhan Nomanpour 5, Mahsa Rasekhian6
, Ebrahim Barzegari2 , Lida Lotfollahi3 , Reza Jafari4 , Bizhan Nomanpour 5, Mahsa Rasekhian6
1- Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
2- Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences Kermanshah, Iran
3- Department of Microbiology and Virology, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran
4- Cellular and Molecular Research Center, Cellular and Molecular Medicine Research Institute, Urmia University of Medical Sciences, Urmia, Iran
5- Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran ,nomanpoursh@gmail.com
6- Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
2- Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences Kermanshah, Iran
3- Department of Microbiology and Virology, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran
4- Cellular and Molecular Research Center, Cellular and Molecular Medicine Research Institute, Urmia University of Medical Sciences, Urmia, Iran
5- Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran ,
6- Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
Abstract: (1491 Views)
Background and Aim: Antibiotic resistance is recognized as a major threat to global health that can result in increased morbidity and mortality. On February 27, 2017, the first list of antibiotic-resistant priority pathogens was published by the World Health Organization (WHO). These pathogens, including Pseudomonas aeruginosa, pose the greatest threat to human health. This research was conducted in order to introduce viable candidate vaccine proteins against P. aeruginosa outer membrane proteins (OMPs) using reverse vaccinology approaches.
Materials and Methods: Fifty-eight clinical isolates and 9982 outer membrane protein sequences were retrieved for vaxign2 calculation of sequence-derived features. First, 30 proteins with the highest adhesion probability were selected, and in the next step, 10 candidates common among the 58 strains with the highest scores were introduced as candidates for further studies. After determining the physicochemical characteristics of these vaccine candidates, the presence of protected domains was predicted in 2 out of 10 proteins.
Results: Based on the results obtained from the bioinformatics analysis of the antigenic properties of these proteins, we identified 10 outer membrane proteins that have the highest antigenic scores, are common among all 58 clinical isolates, have no human protein homologs, and have less than 1 transmembrane helix. These candidate proteins have optimal physicochemical properties. The presence of conserved domains was predicted only in the outer membrane porin F and enterobactin iron receptor.
Conclusion: We suggested 10 candidate proteins that showed suitable characteristics including outer membrane protein F (OprF) and ferric enterobactin receptor.
Keywords: Pseudomonas aeruginosa, Reverse Vaccinology, Antibiotic Resistance, Vaccine Candidate, Outer Membrane Proteins, Bioinformatics
Type of Study: Original Research Article |
Subject:
Microbial Bioinformatics
Received: 2023/06/28 | Accepted: 2023/09/19 | ePublished: 2023/11/29
Received: 2023/06/28 | Accepted: 2023/09/19 | ePublished: 2023/11/29
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