year 17, Issue 4 (July - August 2023)                   Iran J Med Microbiol 2023, 17(4): 396-405 | Back to browse issues page


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Boudiar I, Bouacha M, Besnaci S, Bensaci N, Abdi A, Schweitzer P. Qualitative Melissopalynology Analysis, Glucose Oxydase Activity, and Antibacterial Effect of Honey Samples from Different Botanical Origin. Iran J Med Microbiol 2023; 17 (4) :396-405
URL: http://ijmm.ir/article-1-2036-en.html
1- Department of Biochemistry, Laboratory of Biochemistry and Applied Microbiology, Faculty of Sciences, University Badji Mokhtar, Annaba, Algeria
2- Department of Biochemistry, Laboratory of Biochemistry and Environmental Toxicology, Faculty of Sciences, University Badji Mokhtar, Annaba, Algeria, Algeria , mabrouka.bouacha@univ-annaba.dz, bouacha.mabrouka@yahoo.fr
3- Department of Biology, Laboratory of Cellular Toxicology, Faculty of Sciences, University of Badji Mokhtar, Annaba, Algeria
4- Centre d'Etudes Techniques Apicoles de Moselle (CETAM) Lorraine, Laboratory of Analysis and Beekeeping Ecology, Guénange, France
Abstract:   (567 Views)

Background and Aim: This research aims to study the qualitative melissopalynology analysis, Glucose oxydase (GOX) activity, and the antibacterial effect of honey samples from different botanical and geographical origins.
Materials and Methods: Five natural honey samples were collected from local beekeepers. The antibacterial effect was carried out towards pathogenic bacteria including Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. The antibacterial activity of honey samples was evaluated using six dilutions (80%, 40%, 20%, 10%, 5%, and 2.5%). The bacterial susceptibility to honey was evaluated by the agar well diffusion and broth dilution assays. GOX activity was determined using hydrogen peroxide as a standard with peroxydase and o-dianisidine.
Results: The results showed that two honey samples could be classified as monofloral (H1: Hedysarum coronarium, H4: Ziziphus sp), whereas honey samples H2, H3, and H5 are multifloral honey. All honey exhibit a good bactericidal effect against pathogenic bacteria. Statistical analysis showed that there was a correlation between GOX level and the antibacterial of honey samples.
Conclusion: It can be concluded that honey is an important natural product, which has an antibacterial effect towards pathogenic bacteria. Therefore, honey should be used as an alternative therapy for the treatment of wound infected by multidrug resistant bacteria. Further research should be conducted to evaluate the mechanism and the bioactive compounds in honey.

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Type of Study: Original Research Article | Subject: Medical Bacteriology
Received: 2023/03/21 | Accepted: 2023/07/27 | ePublished: 2023/09/27

References
1. Bouacha M, Besnaci S, Boudiar I, Al-Kafaween MA. Screening of the antibacterial and antibiofilm effect of multifloral honey against multidrug-resistant Pseudomonas aeruginosa. Acta Microbiol Hell. 2022;67:69-79.
2. Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: A review. J Pharm Anal. 2016;6(2):71-9. [DOI:10.1016/j.jpha.2015.11.005] [PMID] [PMCID]
3. Bouacha M, Boudiar I, Akila A, Al-Kafaween MA, Khallef M. The antimutagenic effect of multifloral honey in salmonella/microsomal assay and its correlation with the total polyphenolic content. J Microbiol Biotechnol Food Sci. 2022;11(6):e5557. [DOI:10.55251/jmbfs.5557]
4. Saxena S, Gautam S, Maru G, Kawle D, Sharma A. Suppression of error prone pathway is responsible for antimutagenic activity of honey. Food Chem Toxicol. 2012;50(3-4):625-33. [DOI:10.1016/j.fct.2012.01.003] [PMID]
5. Ahmed S, Sulaiman SA, Baig AA, Ibrahim M, Liaqat S, Fatima S, et al. Honey as a potential natural antioxidant medicine: an insight into its molecular mechanisms of action. Oxidative medicine and cellular longevity. 2018;2018. [DOI:10.1155/2018/8367846] [PMID] [PMCID]
6. Alzahrani HA, Alsabehi R, Boukraâ L, Abde-llah F, Bellik Y, Bakhotmah BA. Antibacterial and Antioxidant Potency of Floral Honeys from Different Botanical and Geographical Origins. Molecules. 2012;17(9):10540-9. [DOI:10.3390/molecules170910540] [PMID] [PMCID]
7. Boudiar I, Bouacha M, Besnaci S, Khallef M, Abdi A. Evaluation of the antibacterial and antimutagenic effects of multiflora honeys produced by the Apis mellifera bee and their correlation with polyphenolic content, flavonoids and color. J Entomol Res. 2022;46(3):607-14. [DOI:10.5958/0974-4576.2022.00105.0]
8. Almasaudi S. The antibacterial activities of honey. Saudi J Biol Sci. 2021(28):2188-96. [DOI:10.1016/j.sjbs.2020.10.017] [PMID] [PMCID]
9. Alvarez-Suarez JM, Tulipani S, Romandini S, Bertoli E, Battino M. Contribution of honey in nutrition and human health: a review. Med J Nutrition Metab. 2010;3:15-23. [DOI:10.1007/s12349-009-0051-6]
10. Mandal MD, Mandal S. Honey: its medicinal property and antibacterial activity. Asian Pac J Trop Biomed. 2011;1(2):154-60. [DOI:10.1016/S2221-1691(11)60016-6] [PMID]
11. Bogdanov S, Lüllmann C, Martin P, von der Ohe W, Russmann H, Vorwohl G, et al. Honey quality and international regulatory standards: review by the International Honey Commission. Bee World. 1999;80(2):61-9. [DOI:10.1080/0005772X.1999.11099428]
12. Bouacha M, Ayed H, Grara N. Honey Bee as Alternative Medicine to Treat Eleven Multidrug-Resistant Bacteria Causing Urinary Tract Infection during Pregnancy. Sci Pharm. 2018;86(2):14. [DOI:10.3390/scipharm86020014] [PMID] [PMCID]
13. Scepankova H, Pinto CA, Paula V, Estevinho LM, Saraiva JA. Conventional and emergent technologies for honey processing: A perspective on microbiological safety, bioactivity, and quality. Compr Rev Food Sci Food Saf. 2021;20(6):5393-420. [DOI:10.1111/1541-4337.12848] [PMID]
14. Abbas HA. Comparative antibacterial and antibiofilm activities of manuka honey and Egyptian clover honey. Asian J Appl Sci. 2014;2(2):110-5.
15. Bucekova M, Jardekova L, Juricova V, Bugarova V, Di Marco G, Gismondi A, et al. Antibacterial activity of different blossom honeys: New findings. Molecules. 2019;24(8):1573. [DOI:10.3390/molecules24081573] [PMID] [PMCID]
16. Cilia G, Fratini F, Marchi M, Sagona S, Turchi B, Adamchuk L, et al. Antibacterial Activity of Honey Samples from Ukraine. Vet Sci. 2020;7(4):181. [DOI:10.3390/vetsci7040181] [PMID] [PMCID]
17. El-Senduny FF, Hegazi NM, Abd Elghani GE, Farag MA. Manuka honey, a unique mono-floral honey. A comprehensive review of its bioactives, metabolism, action mechanisms, and therapeutic merits. Food Biosci. 2021;42:101038. [DOI:10.1016/j.fbio.2021.101038]
18. Lusby PE, Coombes A, Wilkinson JM. Honey: A potent agent for wound healing? J WOCN. 2002;29(6):295-300. https://doi.org/10.1097/00152192-200211000-00008 [DOI:10.1067/mjw.2002.129073]
19. Bouacha M, Besnaci S, Boudiar I, Al-kafaween MA. Impact of Storage on Honey Antibacterial and Antioxidant Activities and their Correlation with Polyphenolic Content. Trop J Nat Prod Res. 2022;6(1):34-9. [DOI:10.26538/tjnpr/v6i1.7]
20. Mercan N, Guvensen A, Celik A, Katircioglu H. Antimicrobial activity and pollen composition of honey samples collected from different provinces in Turkey. Nat Prod Res. 2007;21(3):187-95. [DOI:10.1080/14786410600906277] [PMID]
21. Pasias IN, Kiriakou IK, Kaitatzis A, Koutelidakis AE, Proestos C. Effect of late harvest and floral origin on honey antibacterial properties and quality parameters. Food Chem. 2018;242:513-8. [DOI:10.1016/j.foodchem.2017.09.083] [PMID]
22. Ghanmi M, Aafi A, Satrani B, Aberchane M, Khia A, Yakhlef SEB. Aromatic and medicinal plants in north Africa: opportunities, constraints and prospects. Novel plant bioresources: Applications in food, medicine and cosmetics: Wiley Online Library; 2014. p. 409-23. [DOI:10.1002/9781118460566.ch29]
23. Benaziza-Bouchema D, Schweitzer P. Characterization of the main honeys from the northern regions of Algeria. Cah Agric. 2010;19(6):432-8. [DOI:10.1684/agr.2010.0432]
24. Louveaux J, Maurizio A, Vorwohl G. Methods of Melissopalynology. Bee world. 1978;59(4):139-57. [DOI:10.1080/0005772X.1978.11097714]
25. Albaridi NA. Antibacterial potency of honey. International Journal of Microbiology. 2019;2019. [DOI:10.1155/2019/2464507] [PMID] [PMCID]
26. Cooper RA, Halas E, Molan PC. The efficacy of honey in inhibiting strains of Pseudomonas aeruginosa from infected burns. J Burn Care Res. 2002;23(6):366-70. [DOI:10.1097/00004630-200211000-00002] [PMID]
27. Agbagwa OE, Frank-Peterside N. Effect of raw commercial honeys from Nigeria on selected pathogenic bacteria. Afr J Microbiol Res. 2010;4(16):1801-3.
28. O'Neill AJ, Chopra I. Preclinical evaluation of novel antibacterial agents by microbiological and molecular techniques. Expert Opin Investig Drugs. 2004;13(8):1045-63. [DOI:10.1517/13543784.13.8.1045] [PMID]
29. Johnston M, McBride M, Dahiya D, Owusu-Apenten R, Nigam PS. Antibacterial activity of Manuka honey and its components: An overview. AIMS Microbiol. 2018;4(4):655-64. [DOI:10.3934/microbiol.2018.4.655] [PMID] [PMCID]
30. Syed Yaacob SN, Wahab RA, Huyop F, Lani MN, Zin NM. Morphological alterations in gram-positive and gram-negative bacteria exposed to minimal inhibitory and bactericidal concentration of raw Malaysian stingless bee honey. Biotechnol Biotechnol Equip. 2020;34(1):575-86. [DOI:10.1080/13102818.2020.1788421]
31. Al-Hasani HMH. Study antibacterial activity of honey against some common species of pathogenic bacteria. Iraqi J Sci. 2018;59(A1):30-7. [DOI:10.24996/ijs.2018.59.1A.5]
32. Escuredo O, Silva LR, Valentão P, Seijo MC, Andrade PB. Assessing Rubus honey value: Pollen and phenolic compounds content and antibacterial capacity. Food Chem. 2012;130(3):671-8. [DOI:10.1016/j.foodchem.2011.07.107]
33. Fyfe L, Okoro P, Paterson E, Coyle S, McDougall GJ. Compositional analysis of Scottish honeys with antimicrobial activity against antibiotic-resistant bacteria reveals novel antimicrobial components. Food Sci Technol. 2017;79:52-9. [DOI:10.1016/j.lwt.2017.01.023]
34. Brudzynski K, Kim L. Storage-induced chemical changes in active components of honey de-regulate its antibacterial activity. Food Chem. 2011;126(3):1155-63. [DOI:10.1016/j.foodchem.2010.11.151]
35. Mohapatra DP, Thakur V, Brar SK. Antibacterial efficacy of raw and processed honey. Biotechnol Res Int. 2011;2011:917505. [DOI:10.4061/2011/917505] [PMID] [PMCID]
36. Silhavy TJ, Kahne D, Walker S. The Bacterial Cell Envelope. Cold Spring Harb Perspect. 2010;2(5):a000414. [DOI:10.1101/cshperspect.a000414] [PMID] [PMCID]
37. Paracini N, Schneck E, Imberty A, Micciulla S. Lipopolysaccharides at Solid and Liquid Interfaces: Models for Biophysical Studies of the Gram-negative Bacterial Outer Membrane. Adv Colloid Interface Sci. 2022;301:102603. [DOI:10.1016/j.cis.2022.102603] [PMID]
38. Al-Kafaween MA, Al-Jamal HAN. A comparative study of antibacterial and antivirulence activities of four selected honeys to Manuka honey. Iran J Microbiol. 2022;14(2):238-51. [DOI:10.18502/ijm.v14i2.9193] [PMID] [PMCID]
39. Bouacha M, Besnaci S, Boudiar I. An overview of the most used methods to determine the in vitro antibacterial activity of honey. Acta Microbiol Hell. 2023; 39(1):23-30. [DOI:10.59393/amb23390104]
40. Bouacha M, Besnaci S, Boudiar I. Comparative Study of the Antibacterial Activity of Algerian Honeys and Manuka Honey Toward Pathogenic Bacteria from Burn Wound Infections. Mikrobiolohichnyi Zh. 2023;85(2):26-36. [DOI:10.15407/microbiolj85.02.026]
41. Alaux C, Ducloz F, Crauser D, Le Conte Y. Diet effects on honeybee immunocompetence. Biol Lett. 2010;6(4):562-5. [DOI:10.1098/rsbl.2009.0986] [PMID] [PMCID]
42. Bucekova M, Valachova I, Kohutova L, Prochazka E, Klaudiny J, Majtan J. Honeybee glucose oxidase-its expression in honeybee workers and comparative analyses of its content and H2O2-mediated antibacterial activity in natural honeys. Naturwissenschaften. 2014;101(8):661-70. [DOI:10.1007/s00114-014-1205-z] [PMID]
43. Subramanian R, Umesh Hebbar H, Rastogi NK. Processing of Honey: A Review. Int J Food Prop. 2007;10(1):127-43. [DOI:10.1080/10942910600981708]

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