The Intestinal Zonulin and Zonula Occludens 1 Protein Expression and Lipopolysaccharide Levels In ddY Mice Injected with Dengue Virus Non-Structural Protein 1

Jatmiko, Safari Wahyu; Hartono, Hartono; Ardyanto, Tonang Dwi; Indarto, Dono

doi:10.30699/ijmm.17.4.423

year 17, Issue 4 (July - August 2023) Iran J Med Microbiol 2023, 17(4): 423-431 | Back to browse issues page

‎ 10.30699/ijmm.17.4.423

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Jatmiko S W, Hartono H, Ardyanto T D, Indarto D. The Intestinal Zonulin and Zonula Occludens 1 Protein Expression and Lipopolysaccharide Levels In ddY Mice Injected with Dengue Virus Non-Structural Protein 1. Iran J Med Microbiol 2023; 17 (4) :423-431
URL: http://ijmm.ir/article-1-2004-en.html

The Intestinal Zonulin and Zonula Occludens 1 Protein Expression and Lipopolysaccharide Levels In ddY Mice Injected with Dengue Virus Non-Structural Protein 1

Safari Wahyu Jatmiko

¹, Hartono Hartono²

, Tonang Dwi Ardyanto²

, Dono Indarto²

1- Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia , safari.wahyu@ums.ac.id
2- Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia

Abstract: (818 Views)

Background and Aim: Dengue virus infection remains a health problem. Dengue Virus Non-Structural protein 1 (NS1) increases the release of proinflammatory cytokines that induce intestinal zonulin expression. As a result, the ZO-1 protein translocates to the cytoplasm, which increases enterocyte permeability. This study aimed to investigate the effects of dengue NS1 on intestinal zonulin and ZO-1 expression, intestinal weight, and serum LPS.
Materials and Methods: This experiment used 18 ddY mice with a pre-posttest control group design. Mice were randomly divided into control (C), PBS (T1), and PBS+NS1 (T2) groups. Mice in the T1 and 2 groups were intravenously injected with 500 µL PBS and 50 µg NS1, respectively. Blood samples were collected before and after the three-day treatment. The intestines were weighted directly and were then embedded with formalin for immunostaining. Serum LPS were determined using ELISA. Data were analyzed using paired t-test and ANOVA.
Results: The T2 group had the highest zonulin expression (histoscore=8) compared to the T1 (histoscores=7.33) and C (histoscore=6.33) groups but were not significant (p=0.135). Intestinal ZO-1 expression did not increase in the T groups compared to the C group (p=0.368). The intestine weight in the C group was significantly lower than the T group (p=0.001). After treatment, serum LPS levels in the T2 group were higher (0.34 pg/mL) than before treatment (0.12 pg/mL; p=0.118), but not in the T1 group (p=0.384). Meanwhile, there was a significant decrease in serum LPS levels in the C group (p=0.046).
Conclusion: Injection of 50 µg dengue virus NS1 has a minor effect on intestinal permeability in ddY mice.

Keywords: Dengue Virus, NS1, Zonulin, Zonula Occludens 1, LPS

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Type of Study: Original Research Article | Subject: Medical Virology
Received: 2023/02/13 | Accepted: 2023/07/30 | ePublished: 2023/09/27

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