Comparison induction of inflammatory cytokines production by recombinant Shiga toxin and shiga toxin producing strain

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year 5, Issue 3 (Fall 2011) Iran J Med Microbiol 2011, 5(3): 43-51 | Back to browse issues page

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Comparison induction of inflammatory cytokines production by recombinant Shiga toxin and shiga toxin producing strain. Iran J Med Microbiol 2011; 5 (3) :43-51
URL: http://ijmm.ir/article-1-198-en.html

Comparison induction of inflammatory cytokines production by recombinant Shiga toxin and shiga toxin producing strain

Abstract: (12047 Views)

Shiga toxin (Stx) production by Shiga Toxicogenic Escherichia coli (STEC) is associated with hemolytic uremic syndrome. Stx binds to globotriaosylceramide (Gb3- cer) receptors on eukaryotic cells and promotes endocytosis. It then inhibits protein biosynthesis which triggers apoptosis in the affected cell. Stx has role in apoptosis and immune activity. Stx can induce both apoptosis and necrosis and secretion of cytokine, depending on the cell type. The aim of this study is to assess the effect of recombinant Shiga toxin in induction of inflammatory cytokines in epithelial and monocytic cells. The HeLa and THP-1 cells were used to study the effect of O157 extract and recombinant Shiga toxin. For this purpose 106 cells were cultured in 6 well plates, different concentrations of Shiga toxin were added to each well. Then the cells were collected after 24 hours of incubation. Total RNA was extracted and used in RT-PCR for detection of IL-1a' IL-10' TNF-a and IL-8 cytokines. The affected cells with recombinant Shiga toxin and O157 extract expressed IL-1a and IL-8 mRNA however, TNF-a mRNA was produced in the THP-1 cells.

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Type of Study: Original Research Article | Subject: Medical Bacteriology
Received: 2014/01/2 | Accepted: 2014/01/2 | ePublished: 2014/01/2