Metabolic Parameters and Oral Microbiota in Patients with Atherosclerosis

Nazari, Zahra; Abiri, Ramin; Heidary Moghadam, Reza; Chehri, Goodarz; Alvandi, Amirhooshang; Mohajerani, Hamid reza

doi:10.30699/ijmm.17.3.301

year 17, Issue 3 (May - June 2023) Iran J Med Microbiol 2023, 17(3): 301-308 | Back to browse issues page

‎ 10.30699/ijmm.17.3.301

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Nazari Z, Abiri R, Heidary Moghadam R, Chehri G, Alvandi A, Mohajerani H R. Metabolic Parameters and Oral Microbiota in Patients with Atherosclerosis. Iran J Med Microbiol 2023; 17 (3) :301-308
URL: http://ijmm.ir/article-1-1977-en.html

Metabolic Parameters and Oral Microbiota in Patients with Atherosclerosis

Zahra Nazari¹

, Ramin Abiri²

, Reza Heidary Moghadam³

, Goodarz Chehri⁴

, Amirhooshang Alvandi⁵

, Hamid reza Mohajerani

⁶

1- Department of Microbiology, Arak Branch, Islamic Azad University, Arak, Iran
2- Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
3- Cardiovascular Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
4- Department of Cardiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
5- Medical Technology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
6- Applied neuroscience research center, Arak Branch, Islamic Azad University, Arak, Iran , mohajeranihr@gmail.com

Abstract: (845 Views)

Background and Aim: Oral infections are common among people of any age and can trigger systemic inflammation. The microbiota is a diverse group of microorganisms that play important roles in metabolism, immune function, and homeostasis. Oral microbiota in human atherosclerotic plaques has been identified using various techniques. Therefore, the focus of this study was to determine the correlation between metabolic parameters and oral microbiota composition in patients with atherosclerosis using Denaturing Gradient Gel Electrophoresis (DGGE) assays.
Materials and Methods: In this case-control study, saliva samples were collected from 139 patients with atherosclerosis and healthy individuals from Imam Ali Cardiovascular Hospital, Kermanshah, Iran. After DNA extraction, PCR products were examined and evaluated using DGGE assays.
Results: The study included 89 (36%) patients with a history of atherosclerosis and 50 (36%) healthy individuals. There was a significant relationship between the mean total cholesterol, Low-Density Lipoprotein (LDL), Fasting Blood Sugar (FBS), and Blood Urea Nitrogen (BUN) in the two groups. However, there was no significant difference in the mean high-density lipoprotein (HDL) and Triglyceride levels between the study groups.
Conclusion: Our results showed a relationship between metabolic parameters and oral microbiota composition in patients with atherosclerosis. Additionally, our results indicated that the DGGE assay is a useful method for diagnosing and comparing the oral microbiota of people with atherosclerosis and healthy individuals. Therefore, further examination of the oral microbiota is necessary to determine its potential as a biomarker for atherosclerosis.

Keywords: Metabolic Parameters, Atherosclerosis, Oral microbiota, Denaturing Gradient Gel Electrophoresis

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Type of Study: Original Research Article | Subject: Oral Microbiology
Received: 2023/01/7 | Accepted: 2023/03/11 | ePublished: 2023/06/26

References

1. Liu X-R, Xu Q, Xiao J, Deng Y-M, Tang Z-H, Tang Y-L, et al. Role of oral microbiota in atherosclerosis. Clin Chim Acta. 2020;506:191-5. [DOI:10.1016/j.cca.2020.03.033] [PMID]

2. Libby P, Buring JE, Badimon L, Hansson GK, Deanfield J, Bittencourt MS, et al. Lewis EF. Nat Rev Dis Primers. 2019;5(1):56. [DOI:10.1038/s41572-019-0106-z] [PMID]

3. Pothineni NVK, Subramany S, Kuriakose K, Shirazi LF, Romeo F, Shah PK, et al. Infections, atherosclerosis, and coronary heart disease. Eur Heart J. 2017;38(43):3195-201. [DOI:10.1093/eurheartj/ehx362] [PMID]

4. Yoshida N, Sasaki K, Sasaki D, Yamashita T, Fukuda H, Hayashi T, et al. Effect of Resistant Starch on the Gut Microbiota and Its Metabolites in Patients with Coronary Artery Disease. J Atheroscler Thromb. 2019;26(8):705-19. [DOI:10.5551/jat.47415] [PMID] [PMCID]

5. Plaza-Diaz J, Ruiz-Ojeda FJ, Gil-Campos M, Gil A. Mechanisms of Action of Probiotics. Adv Nutr. 2019;10(suppl_1):S49-S66. [DOI:10.1093/advances/nmy063] [PMID] [PMCID]

6. Paoletti R, Bolego C, Poli A, Cignarella A. Metabolic Syndrome, Inflammation and Atherosclerosis. Vasc Health Risk Manag. 2006;2(2):145-52. [DOI:10.2147/vhrm.2006.2.2.145] [PMID] [PMCID]

7. Eckel RH, Grundy SM, Zimmet PZ. The metabolic syndrome. Lancet. 2005;365(9468):1415-28. [DOI:10.1016/S0140-6736(05)66378-7] [PMID]

8. Mahmoudi H, Hossainpour H. Application and development of fecal microbiota transplantation in the treatment of gastrointestinal and metabolic diseases: A review. Saudi J Gastroenterol. 2023;29(1). [DOI:10.4103/sjg.sjg_131_22] [PMID] [PMCID]

9. Taati Moghadam M, Amirmozafari N, Mojtahedi A, Bakhshayesh B, Shariati A, Masjedian Jazi F. Association of perturbation of oral bacterial with incident of Alzheimer's disease: A pilot study. J Clin Lab Anal. 2022;36(7):e24483. [DOI:10.1002/jcla.24483] [PMID] [PMCID]

10. Chiu B. Multiple infections in carotid atherosclerotic plaques. Am Heart J. 1999;138(5, Supplement):S534-S6. [DOI:10.1016/S0002-8703(99)70294-2] [PMID]

11. Xue L, He J, Gao N, Lu X, Li M, Wu X, et al. Probiotics may delay the progression of nonalcoholic fatty liver disease by restoring the gut microbiota structure and improving intestinal endotoxemia. Sci Rep. 2017;7(1):1-13. [DOI:10.1038/srep45176] [PMID] [PMCID]

12. Beck JS, Kwitek AE, Cogen PH, Metzger AK, Duyk GM, Sheffield VC. A denaturing gradient gel electrophoresis assay for sensitive detection of p53 mutations. Hum Genet. 1993;91(1):25-30. [DOI:10.1007/BF00230217] [PMID]

13. Lüll K, Saare M, Peters M, Kakhiani E, Zhdanova A, Salumets A, et al. Differences in microbial profile of endometrial fluid and tissue samples in women with in vitro fertilization failure are driven by Lactobacillus abundance. Acta Obstet Gynecol Scand. 2022;101(2):212-20. [DOI:10.1111/aogs.14297] [PMID] [PMCID]

14. Alberti KG, Zimmet P, Shaw J. The metabolic syndrome--a new worldwide definition. Lancet. 2005;366(9491):1059-62. [DOI:10.1016/S0140-6736(05)67402-8] [PMID]

15. Aquino ARL, Lima KC, Paiva MS, Rôças IN, Siqueira Jr JF. Molecular survey of atheromatous plaques for the presence of DNA from periodontal bacterial pathogens, archaea and fungi. J Periodont Res. 2011;46(3):303-9. [DOI:10.1111/j.1600-0765.2010.01343.x] [PMID]

16. Watanabe K, Kodama Y, Harayama S. Design and evaluation of PCR primers to amplify bacterial 16S ribosomal DNA fragments used for community fingerprinting. J Microbiol Methods. 2001;44(3):253-62. [DOI:10.1016/S0167-7012(01)00220-2] [PMID]

17. Zangeneh Z, Abdi-Ali A, Khamooshian K, Alvandi A, Abiri R. Bacterial variation in the oral microbiota in multiple sclerosis patients. PloS One. 2021;16(11):e0260384. [DOI:10.1371/journal.pone.0260384] [PMID] [PMCID]

18. Assinger A, Laky M, Schabbauer G, Hirschl AM, Buchberger E, Binder BR, et al. Efficient phagocytosis of periodontopathogens by neutrophils requires plasma factors, platelets and TLR2. J Thromb Haemost. 2011;9(4):799-809. [DOI:10.1111/j.1538-7836.2011.04193.x] [PMID]

19. Fiehn N-E, Larsen T, Christiansen N, Holmstrup P, Schroeder TV. Identification of Periodontal Pathogens in Atherosclerotic Vessels. J Periodontol. 2005;76(5):731-6. [DOI:10.1902/jop.2005.76.5.731] [PMID]

20. Gaetti-Jardim E, Marcelino SL, Feitosa ACR, Romito GA, Avila-Campos MJ. Quantitative detection of periodontopathic bacteria in atherosclerotic plaques from coronary arteries. Int J Med Microbiol. 2009;58(12):1568-75. [DOI:10.1099/jmm.0.013383-0] [PMID]

21. Figuero E, Sánchez-Beltrán M, Cuesta-Frechoso S, Tejerina JM, del Castro JA, Gutiérrez JM, et al. Detection of Periodontal Bacteria in Atheromatous Plaque by Nested Polymerase Chain Reaction. J Periodontol. 2011;82(10):1469-77. [DOI:10.1902/jop.2011.100719] [PMID]

22. Koren O, Spor A, Felin J, Fåk F, Stombaugh J, Tremaroli V, et al. Human oral, gut, and plaque microbiota in patients with atherosclerosis. Proc Natl Acad Sci. 2011;108(supplement_1):4592-8. [DOI:10.1073/pnas.1011383107] [PMID] [PMCID]

23. Sheffield VC, Fishman GA, Beck JS, Kimura AE, Stone EM. Identification of novel rhodopsin mutations associated with retinitis pigmentosa by GC-clamped denaturing gradient gel electrophoresis. Am J Hum Genet. 1991;49(4):699-706.

24. Ott SJ, El Mokhtari NE, Musfeldt M, Hellmig S, Freitag S, Rehman A, et al. Detection of Diverse Bacterial Signatures in Atherosclerotic Lesions of Patients With Coronary Heart Disease. Circulation. 2006;113(7):929-37. [DOI:10.1161/CIRCULATIONAHA.105.579979] [PMID]

25. Abbate A, Toldo S, Marchetti C, Kron J, Van Tassell BW, Dinarello CA. Interleukin-1 and the Inflammasome as Therapeutic Targets in Cardiovascular Disease. Circ Res. 2020;126(9):1260-80. [DOI:10.1161/CIRCRESAHA.120.315937] [PMID] [PMCID]

26. Xian TK, Omar NA, Ying LW, Hamzah A, Raj S, Jaarin K, et al. Reheated Palm Oil Consumption and Risk of Atherosclerosis: Evidence at Ultrastructural Level. Evid Based Complementary Altern Med. 2012;2012:828170. [DOI:10.1155/2012/828170] [PMID] [PMCID]

27. Calandrini CA, Ribeiro AC, Gonnelli AC, Ota-Tsuzuki C, Rangel LP, Saba-Chujfi E, et al. Microbial composition of atherosclerotic plaques. Oral Dis. 2014;20(3):e128-e34. [DOI:10.1111/odi.12205] [PMID]

28. Grau AJ, Becher H, Ziegler CM, Lichy C, Buggle F, Kaiser C, et al. Periodontal Disease as a Risk Factor for Ischemic Stroke. Stroke. 2004;35(2):496-501. [DOI:10.1161/01.STR.0000110789.20526.9D] [PMID]

29. Maarit Niemi R, Heiskanen I, Wallenius K, Lindström K. Extraction and purification of DNA in rhizosphere soil samples for PCR-DGGE analysis of bacterial consortia. J Microbiol Methods. 2001;45(3):155-65. [DOI:10.1016/S0167-7012(01)00253-6] [PMID]

30. Leishman SJ, Lien Do H, Ford PJ. Cardiovascular disease and the role of oral bacteria. J Oral Microbiol. 2010;2(1):5781. [DOI:10.3402/jom.v2i0.5781] [PMID] [PMCID]

31. Kato-Kogoe N, Sakaguchi S, Kamiya K, Omori M, Gu Y-H, Ito Y, et al. Characterization of Salivary Microbiota in Patients with Atherosclerotic Cardiovascular Disease: A Case-Control Study. J Atheroscler Thromb. 2022;29(3):403-21. [DOI:10.5551/jat.60608] [PMID] [PMCID]

32. Bouzid F, Gtif I, Alfadhli S, Charfeddine S, Ghorbel W, Abdelhédi R, et al. A potential oral microbiome signature associated with coronary artery disease in Tunisia. Biosci Rep. 2022;42(7):BSR20220583. [DOI:10.1042/BSR20220583] [PMID] [PMCID]

33. Woodworth Michael H, Neish Emma M, Miller Nancy S, Dhere T, Burd Eileen M, Carpentieri C, et al. Laboratory Testing of Donors and Stool Samples for Fecal Microbiota Transplantation for Recurrent Clostridium difficile Infection. J Clin Microbiol. 2017;55(4):1002-10. [DOI:10.1128/JCM.02327-16] [PMID] [PMCID]

34. Shoemark DK, Allen SJ. The microbiome and disease: reviewing the links between the oral microbiome, aging, and Alzheimer's disease. J Alzheimers Dis. 2015;43(3):725-38. [DOI:10.3233/JAD-141170] [PMID]

35. Azizi M, Motamedi H, Hossainpour H, Abiri R, Kashef M, Ahmadi K, et al. Rapid Detection of vanA Resistance Gene from E. faecalis Clinical Isolates Using Duplex Loop-Mediated Isothermal Amplification and Triplex PCR Assay. Biomed Res Int. 2022;2022. [DOI:10.1155/2022/4384196] [PMID] [PMCID]

36. Dewhirst FE, Chen T, Izard J, Paster BJ, Tanner AC, Yu WH, et al. The human oral microbiome. J Bacteriol. 2010;192(19):5002-17. [DOI:10.1128/JB.00542-10] [PMID] [PMCID]