year 16, Issue 6 (November - December 2022)                   Iran J Med Microbiol 2022, 16(6): 609-614 | Back to browse issues page

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Fakhraddin Raheem T, Ahmed Hasan Ali S. Prevalence and Multi-Drug Resistance Patterns of Uropathogenic E.coli isolated from Women Patients in Kirkuk city, Iraq. Iran J Med Microbiol 2022; 16 (6) :609-614
1- Microbiologist, College of dentistry, AL-Kitab University, Kirkuk, Iraq
2- Microbiologist, College of agriculture Alhawija, Kirkuk University, Kirkuk, Iraq ,
Abstract:   (2256 Views)

Background and Aim: E.coli is a member of Enterobacteriaceae family and gram negative bacilli. Many genus of bacteria can caused urinary tract infection. In fact, uropathogenic E.coli is responsible of 80 - 90% of urinary tract infections in the global. This study was proceeded to determine the prevalence rate of uropathogenic E.coli isolated from Women Patients and study the multi-drug resistance patterns of these isolates, in Kirkuk city, Iraq.
Materials and Methods: Collection of 200 Urine samples from women patients with different ages were achieved over a period January to May 2022, from women's and children's Hospital in Kirkuk city, Iraq. Then inoculated on , Nutrient, MacConkey and blood agar and incubated for 24 hours at 37°C. E.coli colonies were identified by culturing and biochemical tests, then antibiotic susceptibility of these isolates were done.
Results: The prevalence of bacterial positive growth was 132(66%), distributed as the following: E.coli 47(35.6%), S.aureus 37(28.03%), Staphylococcus spp. 9(6.81), Proteus mirabilis 9(6.81), Klebsiella sp. 9(6.81), Pseudomonas aeruginosa 7(5.3%), Streptococcus spp. 7(5.3%), Acinetobacter baumannii 4(3%) and shigella spp. 3(2.27%). Most of E.coli isolates were isolated from women patients with age group 18-38 years. Meropenem is showed the highest effect with no resistant E. coli isolates followed by Amikacin, Ciprofloxacin, Azithromycin, Levofloxacin, Trimethoprim and Nitrofurantoin (10.63%,19.14%,19.14%, 31.91%, 38.29% and 38.29)respectively. In contrast to Augmentin, which revealed no effect towards these isolates with 100% resistance followed by Ceftazidime, Rifampicin and Ceftriaxone (78.72%, 72.34%and 59.57%) respectively. The current data revealed that the isolates were multidrug resistance to four, five, six and seven antimicrobial classes : 18(38.29%), 15(31.91%), 9(19.14%), and 5(10.63%) of E. coli  isolates, respectively.
Conclusion: The sensitivity of all E. coli isolates towards meropenem, calls for attention that the overuse/misuse of this antibiotic will caused the selection pressure and increased the rate of resistance.

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Type of Study: Original Research Article | Subject: Medical Bacteriology
Received: 2022/10/10 | Accepted: 2022/12/2 | ePublished: 2022/12/12

1. Ardakani MA, Ranjbar R. Molecular typing of uropathogenic E. coli strains by the ERIC-PCR method. Electron Physician. 2016;8(4):2291-6. [DOI:10.19082/2291] [PMID] [PMCID]
2. Sabri TS, A. Kareem A. Genotyping Diversity of Echerichia coli isolated from UTI in Iraqi Patients. Med Leg Update. 2020;20(1):1421-7.
3. Magliano E, Grazioli V, Deflorio L, Leuci AI, Mattina R, Romano P, et al. Gender and Age-Dependent Etiology of Community-Acquired Urinary Tract Infections. Sci World J. 2012;2012:349597. [DOI:10.1100/2012/349597] [PMID] [PMCID]
4. Komaroff AL. Urinalysis and urine culture in women with dysuria. Ann Intern Med. 1986;104(2):212-8. [DOI:10.7326/0003-4819-104-2-212] [PMID]
5. Hussein NR, Daniel S, Salim K, Assafi MS. Urinary Tract Infections and Antibiotic Sensitivity Patterns Among Women Referred to Azadi Teaching Hospital, Duhok, Iraq. Avicenna J Clin Microb Infec. 2017;5:27-30. [DOI:10.34172/ajcmi.2018.05]
6. Clinical and Laboratory Standard Institute. Performance Standards for Antimicrobial Susceptibility, Twenty-Fourth Information Supplement, CLSI Document M100-S26. CLSI Wayne PA USA; 2016.
7. Ahmed Hasan S, Najati AM, Abass KS. Prevalence and antibiotic resistance of "pseudomonas aeruginosa" isolated from clinical samples in Kirkuk City, Iraq. Eurasia J Biosci. 2020;14(1):1821-5.
8. Ahmed Hasan S, Fakhraddin Raheem T, Mohammed Abdulla H. Phenotypic, Antibiotyping, and Molecular Detection of Klebsiella Pneumoniae Isolates from Clinical Specimens in Kirkuk, Iraq. Arch Razi Inst. 2021;76(4):1061-7.
9. Abdullah IM. Multiple Drugs Resistance Among Urinary Tract Infection Patients In Duhok City -Kurdistan Region -Iraq. Duhok Med J. 2019;13(1):22-31. [DOI:10.31386/dmj.2019.13.1.3]
10. Nahab HM, Akeel Hamed Al-Oebady M, Aqeel Abdul Munem H. Bacteriological Study of Urinary Tract Infections among Pregnant Women in Al Samawa City of Iraq. Arch Razi Inst. 2022;77(1):107-12.
11. Hasan SA, Abass KS. Prevalence of Gram Negative Bacteria Isolated from Patients with Burn Infection and their Antimicrobial Susceptibility Patterns in Kirkuk City, Iraq. Indian J Public Health Res Dev. 2019;10(8):2197. [DOI:10.5958/0976-5506.2019.02184.3]
12. Ahmed Hasan S, Mohammed Bakr M. Bacteriological and Molecular Detection of Klebsiella oxytoca and its Resistance to Antibiotics among Clinical Specimens from Kirkuk, Iraq. Arch Razi Inst. 2022;77(5):1521-5.
13. Ahmed Hasan S, Saleh I, Ali H. Bacteriological and Molecular Detection of Staphylococcus Aureus and its Resistance to Methicillin among Specimens from Kirkuk Community. Ann Rom Soc Cell Biol. 2021;25(7):461-73.
14. Tutuncu L, Ardic N, Mungen E, Ergur AR, Yergok YZ. Urinary tract infection in pregnancy. Prenat J. 2005;13(4):47.
15. Tahir Jarjees Y, Satam Z. In vitro resistance to cephalosporins in women with bacterial urinary tract infections. Iraqi Postgrad Med J. 2012;11(3):321-5.
16. Kareem IJ, Rasheed IY. Antibiotic susceptibilities of gram negative aerobic bacteria isolated from urinary tract infections in community. Iraqi J Med Sci. 2011;9(4):295-300.
17. Johnson JR, Tiu FS, Stamm WE. Direct antimicrobial susceptibility testing for acute urinary tract infections in women. J Clin Microbiol. 1995;33(9):2316-23. [DOI:10.1128/jcm.33.9.2316-2323.1995] [PMID] [PMCID]
18. Biadglegne F, Abera B. Antimicrobial resistance of bacterial isolates from urinary tract infections at FelgeHiwot Referral Hospital Ethiopia. Ethiop J Health Dev. 2009;23(3):236-8. [DOI:10.4314/ejhd.v23i3.53248]
19. Al-Guranie DR, Al-Mayahie SM. Prevalence of E. coli ST131 among Uropathogenic E. coli Isolates from Iraqi Patients in Wasit Province, Iraq. Int J Microbiol. 2020;2020:8840561. [DOI:10.1155/2020/8840561] [PMID] [PMCID]
20. Assafi MS, Ali FF, Polis RF, Sabaly NJ, Qarani SM. An Epidemiological and Multidrug Resistance Study for E. coli Isolated from Urinary Tract Infection (Three Years of Study). Baghdad Sci J. 2022;19(1): 0007-0007. [DOI:10.21123/bsj.2022.19.1.0007]
21. Ahmed Hasan S, Raoof WM, Kamal Rachid S. A Systematic Review: The Current Status of Carbapenem Resistance In Iraq. World Bull Public Health. 2022;13:88-94.
22. Al-Hilali S, Hussein M. Genetic affinities of multiple drug resistant uropathogenic Escherichia coli isolated from patients with urinary tract infection in Najaf. University of Kufa. 2015.
23. Sanjee SA, Karim ME, Akter T, Parvez MA, Hossain M, Jannat B, et al. Prevalence and antibiogram of bacterial uropathogens of urinary tract infections from a tertiary care hospital of Bangladesh. J Sci Res. 2017;9(3):317-28. [DOI:10.3329/jsr.v9i3.31677]
24. Ahmed Hasan S. Pseudomonas aeruginosa and the multifactorial antibiotic resistance. Eurasian Med Res Periodical. 2022;11:85-94.
25. Lee DS, Lee S-J, Choe H-S. Community-Acquired Urinary Tract Infection by Escherichia coli in the Era of Antibiotic Resistance. Bio Med Res Int. 2018;2018:7656752. [DOI:10.1155/2018/7656752] [PMID] [PMCID]
26. Abbas FM. Prevalence of Transferable OXA-1 β-Lactamase Associated with Carbapenem-Resistant Klebsiella pneumoniae Isolates in Iraq. J Pure Appl Microbiol. 2021; 15(2):877-82. [DOI:10.22207/JPAM.15.2.43]
27. Al-abedi KJ, Al-Mayahi FA. Molecular Detection of Serine Carbapenemase Genes in Carbapenem-Resistant Isolates of Pseudomonas aeruginosa Recovered from Patients in Al-Diwaniyah Province, Iraq. Iraq J Pure Appl Microbiol. 2019;13(3):1775-82. [DOI:10.22207/JPAM.13.3.53]
28. Hussein ZK, Kadhim HS, Hassan JS. Detection of New Delhi Metallo-Beta-Lactamase-1 (blaNDM-1) in Carbapenem-Resistant Pseudomonas aeruginosa Isolated from Clinical Samples in Wasit Hospitals. Iraqi J Med Sci. 2018;16(3):239-46. [DOI:10.22578/IJMS.16.3.3]
29. Gatya Al-Mayahie SM, Al-Guranie DR, Hussein AA, Bachai ZA. Prevalence of common carbapenemase genes and multidrug resistance among uropathogenic Escherichia coli phylogroup B2 isolates from outpatients in Wasit Province/Iraq. PLoS One. 2022; 17(1):e0262984. [DOI:10.1371/journal.pone.0262984] [PMID] [PMCID]

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