Iranian Journal of Medical Microbiology

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The purpose of this project is to propose a method to evaluate Enterobacteriaceae diagnostic kit which is base on multitube micromethod. Differential staining of bacteria can provide preliminary information when a medical technician is attempting to identify an unknown type of infection-causing bacteria. Basic information, such as whether an organism is Gram positive or Gram negative, is used to decide which diagnostic test will be employed to determine the genus and species of the bacterium in question. One of the several devices available commercially for identification of Enterobacteriaceae is the multitube micromethod, a plastic strip holding 10 miniaturized compartments, or cupules, each containing a dehydrated substrate used to determine the metabolic capabilities, and, ultimately, the genus and species of enteric bacteria in the family Enterobacteraceae. After inoculating with a saline suspension of a pure bacterial culture, rehydrating and the strip is then incubated in a small, plastic humidity chamber for 18-24 hours at 37°C. Each tube (an individual test) is assessed for a specific color change indicating the presence of a metabolic reaction that sheds light on the microbes identity. Some of the cupule contents change color due to pH differences, others contain end products that have to be identified using additional reagents. The kit was validated by examining four Enterobacteraceae and comparing its results with available database. The Resuls are a good an agreement and this method is suitable benchmarking

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