year 17, Issue 4 (July - August 2023)
Iran J Med Microbiol 2023, 17(4): 414-422 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Adeyemo O, Okunye O, Nwaokorie F, Kamet O. Isolation and Characterization of Coagulase Positive, Methicillin and Multi-Drug Resistant Staphylococcus and Mammaliicoccus species Isolated from Wound of Patients Attending Federal Medical Centre, Yola, Adamawa State, Nigeria. Iran J Med Microbiol 2023; 17 (4) :414-422
URL: http://ijmm.ir/article-1-1860-en.html
URL: http://ijmm.ir/article-1-1860-en.html
1- Department of Biotechnology, Faculty of Life Sciences, Modibbo Adama University, Yola, Adamawa State, Nigeria , omadeyemo@mautech.edu.ng
2- Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Olabisi Onabanjo University, Ago Iwoye, Ogun State, Nigeria
3- Department of Medical Laboratory Science, College of Medicine, University of Lagos, Lagos State, Nigeria
4- Department of Microbiology, Faculty of Life Sciences, Modibbo Adama University, Yola, Adamawa State, Nigeria
2- Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Olabisi Onabanjo University, Ago Iwoye, Ogun State, Nigeria
3- Department of Medical Laboratory Science, College of Medicine, University of Lagos, Lagos State, Nigeria
4- Department of Microbiology, Faculty of Life Sciences, Modibbo Adama University, Yola, Adamawa State, Nigeria
Abstract: (718 Views)
Background and Aim: The treatment of bacterial infections especially of the family Staphylococcaceae is a major health burden that has led to economic losses, high morbidity and mortality rates globally. This study aimed at isolation and characterization of coagulase positive, methicillin and multi-drug resistant Staphylococci isolated from wounds of patients.
Materials and Methods: Forty-five wound swabs were collected using sterile swab sticks. Isolation was done by streaking technique on mannitol salt agar. The isolates obtained were screened for methicillin and multi-drug resistance using disk diffusion method. Biochemical and molecular characteristics using 16S rDNA gene were used to identify the selected isolates.
Results: Four Staphylococci isolates resistant to methicillin (0.00 mm to 9.0 mm) were selected out of thirty-one. Reactions to catalase and coagulase productions were positive. Three out of the four isolates were identified using their 16S rDNA genes and they were found to be closely related to other family members of Staphylococcaceae at GenBank. The fourth isolate was identified using its biochemical characteristics as Staphylococcus sp. HFS4. The three identified isolates were M. sciuri HFS1 (ON340756), M. sciuri HFS3 (ON340770) and S. haemolyticus HFS2 (ON35435). The four isolates were resistant to more than five antibiotics that cut across all the classes of antibiotics. The multiple antimicrobial indices were between 0.5 and 0.8.
Conclusion: There is need for regular antimicrobial resistance surveillance within the hospital environment, earlier detection and correct prescription of potent antimicrobials will check the spread of Staphylococci infections and their virulent genes.
Keywords: Antimicrobial surveillance, Methicillin and Multi-drug resistance, Mammalicoccus sciuri, Staphylococcus haemolyticus, Coagulase Positive Staphylococci
Type of Study: Original Research Article |
Subject:
Antibiotic Resistance
Received: 2023/01/15 | Accepted: 2023/06/19 | ePublished: 2023/09/27
Received: 2023/01/15 | Accepted: 2023/06/19 | ePublished: 2023/09/27
References
1. Bierowiec K, Korzeniowska-Kowal A, Wzorek A, Rypuła K, Gamian A. Prevalence of Staphylococcus Species Colonization in Healthy and Sick Cats. Biomed Res Int. 2019;2019:4360525. [DOI:10.1155/2019/4360525] [PMID] [PMCID]
2. Lienen T, Schnitt A, Hammerl JA. Mammaliicoccus spp. from German Dairy Farms Exhibit a Wide Range of Antimicrobial Resistance Genes and Non-Wildtype Phenotypes to Several Antibiotic Classes. Biol. 2022;11(2):152. [DOI:10.3390/biology11020152] [PMID] [PMCID]
3. Shivaee A, Rajabi S, Farahani HE, Imani Fooladi AA. Effect of sub-lethal doses of nisin on Staphylococcus aureus toxin production and biofilm formation. Toxicon. 2021;197:1-5. [DOI:10.1016/j.toxicon.2021.03.018] [PMID]
4. Madhaiyan M, Wirth JS, Saravanan VS. Phylogenomic analyses of the Staphylococcaceae family suggest the reclassification of five species within the genus Staphylococcus as heterotypic synonyms, the promotion of five subspecies to novel species, the taxonomic reassignment of five Staphylococcus species to Mammaliicoccus gen. nov., and the formal assignment of Nosocomiicoccus to the family Staphylococcaceae. Int J Syst Evol Microbiol. 2020;70(11):5926-36. [DOI:10.1099/ijsem.0.004498] [PMID]
5. Götz F, Bannerman T, Schleifer KH. The Genera Staphylococcus and Macrococcus. Prokaryotes. 2006:5-75. [DOI:10.1007/0-387-30744-3_1] [PMCID]
6. Nemeghaire S, Argudín MA, Feßler AT, Hauschild T, Schwarz S, Butaye P. The ecological importance of the Staphylococcus sciuri species group as a reservoir for resistance and virulence genes. Vet Microbiol. 2014;171(3-4):342-56. [DOI:10.1016/j.vetmic.2014.02.005] [PMID]
7. Marincola G, Liong O, Schoen C, Abouelfetouh A, Hamdy A, Wencker FDR, et al. Antimicrobial Resistance Profiles of Coagulase-Negative Staphylococci in Community-Based Healthy Individuals in Germany. Front Public Health. 2021;9:684456. [DOI:10.3389/fpubh.2021.684456] [PMID] [PMCID]
8. Barber M. Methicillin-resistant staphylococci. J Clin Pathol. 1961;14(4):385-93. [DOI:10.1136/jcp.14.4.385] [PMID] [PMCID]
9. International Working Group on the Classification of Staphylococcal Cassette Chromosome Elements (IWG-SCC). Classification of Staphylococcal Cassette Chromosome mec (SCCmec): Guidelines for Reporting Novel SCCmec Elements. Antimicrob Agents Chemother. 2009;53(12):4961-7. [DOI:10.1128/AAC.00579-09] [PMID] [PMCID]
10. Shivaee A, Sadeghi Kalani B, Talebi M, Darban-Sarokhalil D. Does biofilm formation have different pathways in Staphylococcus aureus?. Iran J Basic Med Sci. 2019;22(10):1147-52.
11. Belhout C, Elgroud R, Butaye P. Methicillin-Resistant Staphylococcus aureus (MRSA) and Other Methicillin-Resistant Staphylococci and Mammaliicoccus (MRNaS) Associated with Animals and Food Products in Arab Countries: A Review. Vet Sci. 2022;9(7):317. [DOI:10.3390/vetsci9070317] [PMID] [PMCID]
12. Schauer B, Szostak MP, Ehricht R, Monecke S, Feßler AT, Schwarz S, et al. Diversity of methicillin-resistant coagulase-negative Staphylococcus spp. and methicillin-resistant Mammaliicoccus spp. isolated from ruminants and New World camelids. Vet Microbiol. 2021;254:109005. [DOI:10.1016/j.vetmic.2021.109005] [PMID]
13. Sadigh-Eteghad S, Dehnad A, Shanebandi D, Khalili I, Razmarayii N, Namvaran A. Identification and characterization of a Streptomyces sp. isolate exhibiting activity against multidrug-resistant coagulase-negative Staphylococci. Vet Res Commun. 2011;35(8):477-86. [DOI:10.1007/s11259-011-9491-9] [PMID]
14. Mapara N, Sharma M, Shriram V, Bharadwaj R, Mohite KC, Kumar V. Antimicrobial potentials of Helicteres isora silver nanoparticles against extensively drug-resistant (XDR) clinical isolates of Pseudomonas aeruginosa. Appl Microbiol Biotechnol. 2015;99(24):10655-67. [DOI:10.1007/s00253-015-6938-x] [PMID]
15. Bardasheva A, Tikunov A, Kozlova Y, Zhirakovskaia E, Fedorets V, Fomenko N, et al. Antibiotic Resistance and Pathogenomics of Staphylococci Circulating in Novosibirsk, Russia. Microorganisms. 2021;9(12):2487. [DOI:10.3390/microorganisms9122487] [PMID] [PMCID]
16. McClure JA, Conly JM, Obasuyi O, Ward L, Ugarte-Torres A, Louie T, et al. A Novel Assay for Detection of Methicillin-Resistant Staphylococcus aureus Directly From Clinical Samples. Front Microbiol. 2020;11:1295. [DOI:10.3389/fmicb.2020.01295] [PMID] [PMCID]
17. Takeuchi F, Watanabe S, Baba T, Yuzawa H, Ito T, Morimoto Y, et al. Whole-Genome Sequencing of Staphylococcus haemolyticus Uncovers the Extreme Plasticity of Its Genome and the Evolution of Human-Colonizing Staphylococcal Species. J Bacteriol. 2005;187(21):7292-308. [DOI:10.1128/JB.187.21.7292-7308.2005] [PMID] [PMCID]
18. Hudzicki J. Kirby-Bauer disk diffusion susceptibility test protocol. Am Soc Microbiol. 2009;15:55-63.
19. Wayne P. Clinical and Laboratory Standards Institute (CLSI) method for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard-8th ed, CLSI document M07-A8, USA; 2009.
20. Krumperman PH. Multiple antibiotic resistance indexing of Escherichia coli to identify high-risk sources of fecal contamination of foods. Appl Environ Microbiol. 1983;46(1):165-70. [DOI:10.1128/aem.46.1.165-170.1983] [PMID] [PMCID]
21. Fawole M, Oso B. Characterization of bacteria: Laboratory manual of microbiology. Spectrum Book Ltd, Ibadan, Nigeria. 242004.
22. Shanehbandi D, Baradaran B, Sadigh-Eteghad S. Occurrence of Methicillin Resistant and Enterotoxigenic Staphylococcus aureus in Traditional Cheeses in the North West of Iran. Int Sch Res. 2014;2014:129580. [DOI:10.1155/2014/129580] [PMID] [PMCID]
23. Sanger F, Coulson AR. A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase. J Mol Biol. 1975;94(3):441-8. [DOI:10.1016/0022-2836(75)90213-2] [PMID]
24. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol Biol Evol. 2018;35(6):1547-9. [DOI:10.1093/molbev/msy096] [PMID] [PMCID]
25. Fernandes Queiroga Moraes G, Cordeiro LV, de Andrade Júnior FP. Main laboratory methods used for the isolation and identification of Staphylococcus spp. Rev Colomb Cienc Quim-Farm. 2021;50(1):5-28. [DOI:10.15446/rcciquifa.v50n1.95444]
26. Sands K, Carvalho MJ, Spiller OB, Portal EAR, Thomson K, Watkins WJ, et al. Characterisation of Staphylococci species from neonatal blood cultures in low- and middle-income countries. BMC Infect Dis. 2022;22(1):593. [DOI:10.1186/s12879-022-07541-w] [PMID] [PMCID]
27. Osada M, Aung MS. Prevalence and Antimicrobial Resistance of Staphylococcus aureus and Coagulase-Negative Staphylococcus/ Mammaliicoccus from Retail Ground Meat: Identification of Broad Genetic Diversity in Fosfomycin Resistance Gene fosB. Pathogens. 2022;11(4):496. [DOI:10.3390/pathogens11040469] [PMID] [PMCID]
28. Sadiq A, Samad M, Saddam, Basharat N, Ali S, Roohullah, et al. Methicillin-Resistant Staphylococcus aureus (MRSA) in Slaughter Houses and Meat Shops in Capital Territory of Pakistan During 2018-2019. Front Microbiol. 2020;11:577707. [DOI:10.3389/fmicb.2020.577707] [PMID] [PMCID]
29. Mir R, Salari S. Determination of frequency, multiple antibiotic resistance index and resistotype of Salmonella spp. in chicken meat collected from southeast of Iran. Vet Med Sci. 2022;8(1):229-36. [DOI:10.1002/vms3.647] [PMID] [PMCID]
30. Asante J, Hetsa BA, Amoako DG. Multidrug-Resistant Coagulase-Negative Staphylococci Isolated from Bloodstream in the uMgungundlovu District of KwaZulu-Natal Province in South Africa: Emerging Pathogens. Antibiotics. 2021;10(2):198. [DOI:10.3390/antibiotics10020198] [PMID] [PMCID]
31. Javid F, Taku A, Bhat MA, Badroo GA, Mudasir M, Sofi TA. Molecular typing of Staphylococcus aureus based on coagulase gene. Vet world. 2018;11(4):423-30. [DOI:10.14202/vetworld.2018.423-430] [PMID] [PMCID]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Copyright Policy
Iranian Journal of Medical Microbiology by Farname is licensed under CC BY-NC 4.0
