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1- Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran
2- Clinical Tuberculosis and Epidemiology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- Biostatistics Department, Mycobacteriology Research Center, National Research Institute of Tuberculosis and lung Diseases, Masih Daneshvari Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4- Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Iran.
5- Department of Cell and Molecular Biology, School of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran
6- Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran , mohamadnia.ar@gmail.com
Abstract:   (216 Views)
Background and Aim: Carbapenem-resistant Enterobacteriaceae (CRE) are rapidly growing, which makes it vital to detect antibacterial activity. The carbapenem family does not have an automated antimicrobial susceptibility testing card, however. So the aim of this study was to identify the prevalence of carbapenemase-producing strains of Gram-negative bacteria and determine their antibiotic susceptibility pattern in Tehran, Iran.
Materials and Methods: In this cross-sectional study, between 2019 and 2020, 1600 samples were taken from the Masih Daneshvari hospital's laboratory Iran. Utilizing standard biochemical methods, all isolated bacteria were identified. The common Kirby-Bauer Disc diffusion method was used for the antimicrobial susceptibility testing. Real-time polymerase chain reaction was used to evaluate the molecular detection of genes producing carbapenemase.
Results:  Of 1502 (94.7) Gram-negative bacilli, 37.3% isolates were Pseudomonas aeruginosa, 30.6% Acinetobacter baumannii, 16.5% Klebsiella, 9.3% Escherichia coli0.6% Pseudomonas multophila,0.4% Neisseria1105 (73.5%) isolates were carbapenem-sensitive, while the remaining 397 (26.5%) isolates were carbapenem-resistant. Molecular testing of this sample showed that 80% oo tested isolates had resistance genes to at least one antibiotic resistance gene. The following carbapenemase genes were most frequently detected among resistant strains: blaimp (35%), blavim (20%), blakpc (15%), blaoxa -48 (10%), blandm (10%), and blages (10%).
Conclusion:  From this study authors can conclude that carbapenemase-producing Enterobacteriaceae is increasing in Iran and the use of phenotypic methods for detection of CPEs showed good sensitivity. Before prescribing antibiotics to patients, this test should be performed.
Article number: 10
     
Type of Study: Original Research Article | Subject: Medical Bacteriology
Received: 2022/07/7 | Accepted: 2022/08/18 | ePublished: 2022/09/9

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