Diphtheria Toxin Repressor (dtxR) Gene-Based Genetic Diversity of Corynebacterium diphtheriae Isolated in Jakarta, Indonesia, 2018–2019

Yasmon, Andi; Evriarti, Paulina Rosa; Rosanna, Yeva

doi:10.30699/ijmm.17.2.262

year 17, Issue 2 (March - April 2023) Iran J Med Microbiol 2023, 17(2): 262-266 | Back to browse issues page

‎ 10.30699/ijmm.17.2.262

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Yasmon A, Evriarti P R, Rosanna Y. Diphtheria Toxin Repressor (dtxR) Gene-Based Genetic Diversity of Corynebacterium diphtheriae Isolated in Jakarta, Indonesia, 2018–2019. Iran J Med Microbiol 2023; 17 (2) :262-266
URL: http://ijmm.ir/article-1-1795-en.html

Diphtheria Toxin Repressor (dtxR) Gene-Based Genetic Diversity of Corynebacterium diphtheriae Isolated in Jakarta, Indonesia, 2018–2019

Andi Yasmon

¹, Paulina Rosa Evriarti²

, Yeva Rosanna³

1- Department of Microbiology, Faculty of Medicine, Universitas Indonesia/Cipto Mangunkusumo Hospital. Jl.Pegangsaan Timur 16, Cikini, Jakarta Pusat 10320, Jakarta,Indonesia , andiyasmon@gmail.com
2- Biomedical Science Graduate Program, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.5, Jakarta Pusat 10320, Jakarta, Indonesia
3- Department of Microbiology, Faculty of Medicine, Universitas Indonesia/Cipto Mangunkusumo Hospital. Jl.Pegangsaan Timur 16, Cikini, Jakarta Pusat 10320, Jakarta, Indonesia

Abstract: (1142 Views)

Background and Aim: In Indonesia, diphtheria cases caused by Corynebacterium diphtheriae are still occurring. One of the causes is probably the diphtheria toxin repressor (dtxR) gene, which influences toxin expression. In this study, the gene was characterized to determine the mutations that affect the DtxR protein.
Materials and Methods: The dtxR genes of 10 C. diphtheria strains isolated in Jakarta were amplified by conventional PCR. The PCR products were directed to DNA sequencing by using overlapping primers.
Results and Conclusion: No mutations showed amino acid changes. We propose that the DtxR protein is conserved among bacterial strains isolated from Jakarta.

Keywords: Corynebacterium diphtheriae, dtxR, Mutation, Phylogeny tree

Full-Text [PDF 450 kb] (350 Downloads) | | Full-Text (HTML) (350 Views)

Type of Study: Brief Original Article | Subject: Molecular Microbiology
Received: 2022/07/27 | Accepted: 2022/12/23 | ePublished: 2023/03/30

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