year 17, Issue 2 (March - April 2023)                   Iran J Med Microbiol 2023, 17(2): 262-266 | Back to browse issues page

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Yasmon A, Evriarti P R, Rosanna Y. Diphtheria Toxin Repressor (dtxR) Gene-Based Genetic Diversity of Corynebacterium diphtheriae Isolated in Jakarta, Indonesia, 2018–2019. Iran J Med Microbiol 2023; 17 (2) :262-266
1- Department of Microbiology, Faculty of Medicine, Universitas Indonesia/Cipto Mangunkusumo Hospital. Jl.Pegangsaan Timur 16, Cikini, Jakarta Pusat 10320, Jakarta,Indonesia ,
2- Biomedical Science Graduate Program, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.5, Jakarta Pusat 10320, Jakarta, Indonesia
3- Department of Microbiology, Faculty of Medicine, Universitas Indonesia/Cipto Mangunkusumo Hospital. Jl.Pegangsaan Timur 16, Cikini, Jakarta Pusat 10320, Jakarta, Indonesia
Abstract:   (990 Views)

Background and Aim: In Indonesia, diphtheria cases caused by Corynebacterium diphtheriae are still occurring. One of the causes is probably the diphtheria toxin repressor (dtxR) gene, which influences toxin expression. In this study, the gene was characterized to determine the mutations that affect the DtxR protein.
Materials and Methods: The dtxR genes of 10 C. diphtheria strains isolated in Jakarta were amplified by conventional PCR. The PCR products were directed to DNA sequencing by using overlapping primers.
Results and Conclusion: No mutations showed amino acid changes. We propose that the DtxR protein is conserved among bacterial strains isolated from Jakarta.

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Type of Study: Brief Original Article | Subject: Molecular Microbiology
Received: 2022/07/27 | Accepted: 2022/12/23 | ePublished: 2023/03/30

1. Sharma NC, Efstratiou A, Mokrousov I, Mutreja A, Das B, Ramamurthy T. Diphtheria (Primer). Nat Rev Dis Primers. 2019;5(1). [DOI:10.1038/s41572-019-0131-y] [PMID]
2. Clarke KEN. Review of the epidemiology of diphtheria 2000-2016. World Health Organ. 2017:2021-05.
3. Karyanti MR, Nelwan EJ, Assyidiqie IZ, Satari HI, Hadinegoro SR. Diphtheria epidemiology in Indonesia during 2010-2017. Acta Med Indones. 2019;51(3):205-13.
4. Hughes GJ, Mikhail AFW, Husada D, Irawan E, Kafatos G, Bracebridge S, et al. Seroprevalence and determinants of immunity to diphtheria for children living in two districts of contrasting incidence during an outbreak in East Java, Indonesia. Pediatr Infect Dis J. 2015;34(11):1152-6. [DOI:10.1097/INF.0000000000000846] [PMID]
5. Fitriansyah A. The description of Diphtheria immunization history to Diphtheria patients in Surabaya at 2017. J Berk Epidemiol. 2018;6(2):103. [DOI:10.20473/jbe.V6I22018.103-111]
6. WHO. Immunization, Vaccine, and Biologicas Diphtheriae. 2019.
7. Nakao H, Mazurova IK, Glushkevich T, Popovic T. Analysis of heterogeneity of Corynebacterium diphtheriae toxin gene, tox, and its regulatory element, dtxR, by direct sequencing. Res Microbiol. 1997;148(1):45-54. [DOI:10.1016/S0923-2508(97)81899-2] [PMID]
8. Mulyastuti Y, Rahayu SI, Sunarno S, Wasito EB. Investigation of Diphtheria in Indonesia: dtxR and tox genes analysis of corynebacterium diphtheriae collected from outbreaks. Biodivers J Biol Divers. 2017;18(2):784-7. [DOI:10.13057/biodiv/d180247]
9. Sabbadini PS, Genovez MRN, Silva CFd, Adelino TLN, Santos CSd, Pereira GA, et al. Fibrinogen binds to nontoxigenic and toxigenic Corynebacterium diphtheriae strains. Mem Inst Oswaldo Cruz. 2010;105:705-11. [DOI:10.1590/S0074-02762010000500018] [PMID]
10. Love JF, VanderSpek JC, Marin V, Guerrero L, Logan TM, Murphy JR. Genetic and biophysical studies of diphtheria toxin repressor (DtxR) and the hyperactive mutant DtxR(E175K) support a multistep model of activation. Proc Natl Acad Sci. 2004;101(8):2506-11. [DOI:10.1073/pnas.0303794101] [PMID] [PMCID]
11. Kolodkina VL, Titov LP, Sharapa TN, Drozhzhina ON. Point mutations in tox promoter/operator and diphtheria toxin repressor (DTXR) gene associated with the level of toxin production by Corynebacterium diphtheriae strains isolated in Belarus. Mol Gen Mikrobiol Virusol. 2007(1):22-9. [DOI:10.3103/S0891416807010041]
12. Siu K, Oiu B, Mel'nikov VG, Gubina NI, Loseva LV, Mazurova IK. Polymorphism of tox and dtxR genes in circulating strains of Corynebacterium diphtheriae. Zh Mikrobiol Epidemiol Immunobiol. 2009(1):7-11.
13. Sunarno S, Mulyastuti Y, Puspandari N, Sariadji K. DNA sequence analysis of dtxR gene (partial) of Corynebacterium diphtheriae causing diphtheria in Jawa and Kalimantan Islands, Indonesia. Indones Biomed J. 2017;9(2):91-8. [DOI:10.18585/inabj.v9i2.268]
14. Yasmon A, Rosana Y, Usman D, Prilandari LI, Hartono TS. Identification and phylogenetic analysis of Corynebacterium diphtheriae isolates from Jakarta, Indonesia based on partial rpoB gene. Biodivers J Biol Divers. 2020;21(7). [DOI:10.13057/biodiv/d210726]
15. Rosana Y, Prilandari LI, Ajisman R, Hartono TS, Yasmon A. Detection of toxin-producing Corynebacterium diphtheriae from throat swabs of diphtheria patients using duplex real-time PCR. Iran J Microbiol. 2020;12(6):508-15. [DOI:10.18502/ijm.v12i6.5024] [PMID] [PMCID]
16. Zasada AA, Mosiej E. Contemporary microbiology and identification of Corynebacteria spp. causing infections in human. Lett Appl Microbiol. 2018;66(6):472-83. [DOI:10.1111/lam.12883] [PMID]
17. du Plessis M, Wolter N, Allam M, de Gouveia L, Moosa F, Ntshoe G, et al. Molecular Characterization of Corynebacterium diphtheriae Outbreak Isolates, South Africa, March-June 2015. Emerg Infect Dis. 2017;23(8):1308-15. [DOI:10.3201/eid2308.162039] [PMID] [PMCID]
18. Wang Z, Schmitt MP, Holmes RK. Characterization of mutations that inactivate the diphtheria toxin repressor gene (dtxR). Infect Immun. 1994;62(5):1600-8. [DOI:10.1128/iai.62.5.1600-1608.1994] [PMID] [PMCID]

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