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1- Department of Microbiology, Ayatollah Amoli Branch, Islasmic Azad university, Amol, Iran
2- Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran , srmohebbi@gmail.com
3- Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4- Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract:   (200 Views)
Background and Aim: Circulating microRNAs have the potential to serve as biomarkers in diagnostics and monitoring of diseases progression, including liver diseases. Therefore, this study investigated the alteration in the expression levels of microRNA-222 (miR-222) in patients with chronic hepatitis B (CHB) as a potential diagnostic biomarker.
Materials and Methods: MiR-222 expression was analyzed in the 86 plasma samples, including 43 patients with CHB and 43 healthy individuals as control group. RNA extraction and cDNA synthesis processes were done and then, the expression of the miR-222 was measured by qRT-PCR. The results were analyzed by the Mann-Whitney U-test Spearman to show the correlation between miR-222 and clinical parameters.
Results: MiR-222 had difference in expression levels between the patient and control groups (miRNA-222 Fold change= 1.384), nevertheless a statically significant difference was not observed (p value=0.269).
Conclusion: Our study showed that despite changes in miR-222 expression levels in the group of patients with chronic hepatitis B compared to the healthy group, it cannot be utilized as a precise diagnostic biomarker and more studies, in a wider scale, are needed to determine the role of this microRNA in patients with CHB
Article number: 6
     
Type of Study: Original Research Article | Subject: Medical Virology
Received: 2022/05/19 | Accepted: 2022/07/3 | ePublished: 2022/09/9

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