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1- Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
2- Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
3- 3Department of Pharmacology, Hamadan University of Medical Sciences, Hamadan, Iran
4- Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
5- Department of Biological Sciences, National University of Medical Sciences (NUMS), Rawalpindi, Pakistan
6- Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran ,
Abstract:   (181 Views)
Background& Objective: Uropathogenic Escherichia coli (UPEC) is the most prevalent causative agent of urinary tract infections (UTIs) in both community and hospital settings. Annually about 150 million people globally develop UTIs, resulting in increased healthcare costs. The current study examined the identification and the frequency distribution of virulence factors among fluoroquinolones-resistant (FQs-R) and fluoroquinolones-susceptible (FQs-S) UPEC strains in Hamadan hospitals, west of Iran.
Methods: One hundred-seventy urine samples were collected consecutively from inpatients at three different hospitals in Hamadan city from March to September 2018.  The UPEC isolates were identified using biochemical tests and polymerase chain reaction (PCR). The antimicrobial susceptibility and the minimum inhibitory concentration (MIC) of Ciprofloxacin were determined by the disk diffusion and the broth microdilution methods, respectively. The prevalence of pap, aer, and hly genes was investigated by a multiplex-PCR.
Results: Among 170 urine samples collected from inpatients, E.coli was the most common isolate with a frequency of 125 (73.5%).  Resistance to Q (Nalidixic acid) and FQs including Ciprofloxacin, Norfloxacin, and Ofloxacin was detected in 88.8%, 71.2%, 70.4%, and 68.8% of UPEC isolates, respectively. The prevalence of hly and pap genes in FQs-R strains was significantly lower than in FQs-S strains.
Conclusion: The high-level antibiotic resistance to quinolones & fluoroquinolones and heterogeneity of virulence genes among clinical UPEC isolates need strong attention
Article number: 11
Type of Study: Original Research Article | Subject: Medical Bacteriology
Received: 2022/03/14 | Accepted: 2022/06/29 | ePublished: 2022/09/9

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