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1- Department of Biology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
2- Department of Biology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran , rabi-izadiamoli@yahoo.com
3- Infectious Disease and Tropical Medicine Research Center, Health Research Center, Babol University of Medical Sciences, Babol, Iran
Abstract:   (206 Views)
Background and Objective: Carbapenem resistant- Pseudomonas aeruginosa (CRPA) is one of the most important causes of severe and persistent infections. The contributions of different mechanisms of resistance to Carbapenems and biofilm formation among a collection of imipenem susceptible and non-susceptible P. aeruginosa isolates were investigated.

Method: In this cross-sectional study, a total of 117 P. aeruginosa isolates were collected. Susceptibility of isolates to various antimicrobials was assessed by disc diffusion method. Carbazole method was used for detection of alginate producers. Multiplex-PCRs was performed for detection of biofilm and resistance genes. The expression mRNA levels of efflux pumps were assessed by phenotypic and genotypic (Quantitative Real-time PCR) approaches. 

Results: The highest resistance rate was related to ceftazidime, chloramphenicol, ceftriaxone, tetracycline and levofloxacin. MDR phenotype was observed in 8.4% of strains. The frequency of carbapenem resistance was also 24.7%. Carbazole test was positive in 53.8%. In general, 62.4% of isolates were able to form biofilm, 28.8% of which were resistant to carbapenem. The distribution of algD and algU genes were 41.8% and 26.5%, respectively. The frequency of MBL-encoded genes were as follows; blaIMP (62.1%), blaVIM (31.0%), and blaNDM (6.8%). The relative levels of MexX, MexC, MexB and MexA mRNA in CRPA strains with active efflux pump was 81.8%, 63.6%, 54.5% and 36.4%, respectively.

Conclusion: The existence of different resistant mechanisms in P. aeruginosa can cause cross antibiotic resistance, lead to the appearance of resistant strains, and make the treatment difficult. Biofilm production is directly related to antibiotic resistance. Efflux pumps are actively expressed in carbapenem-resistant strains
Article number: 8
     
Type of Study: Original Research Article | Subject: Medical Bacteriology
Received: 2022/03/10 | Accepted: 2022/06/24 | ePublished: 2022/09/9

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