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1- Department of Microbiology, Faculty of Basic Science Qom Branch, Islamic Azad University, Qom, Iran
2- Department of Microbiology, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran , Soleimanidor@yahoo.com
3- Department of Cardiology, School of Medicine, AJA University of Medical Sciences, Tehran, Iran
4- Department of Infectious Disease, School of Aerospace and Subaquatic Medicine, Beasat Hospital, AJA University of Medical Sciences, Tehran, Iran
5- Department of Genetics and Advanced Medical Technology, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran
6- Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran.
Abstract:   (237 Views)
Background and Aim: Coxiella burnetii is a mandatory gram-negative and intracellular bacterium. The most common pathogens are Query Fever in humans and Coxiellosis in animals. Due to the variety of ways of infection and the lack of accurate, comprehensive, and reliable research, and the lack of statistics on the percentage of infection with C. burnetiii and the high resistance of this bacterium in the environment, it is important to investigate the presence of nosocomial infections with this bacterium. Therefore, the aim of this study was to molecularly detect C. burnetiii bacteria that cause infective endocarditis in military hospitals.
Materials and Methods: For this purpose, 100 negatively cultured endocarditis specimens were collected, and DNA was extracted from C. burnetii with an extraction kit. Also, a PCR reaction was performed on the genome of negative control samples to evaluate the specificity of primers and determine the method's specificity. After ligation steps, JM107 E. coli susceptible to calcium chloride was used to accept the plasmid containing the gene.
Results:  In quantitative DNA analysis, its amount was calculated between 1.69 and 1.8. The last dilution of the PUC18 plasmid for C. burnetii with an initial concentration of 700 ng / µl, which formed a detectable band on the gel, was calculated to be 7-10, and the minimum number of detectable copies in a 25 μL PCR reaction equal to 22 copies.
Conclusion:  Although C. burnetii was not detected in any of the blood samples in this study. Naturally, however, C. burnetii has a broad and varied host spectrum in the Epizootic and Enzootic foci.
Article number: 13
     
Type of Study: Original Research Article | Subject: Medical Bacteriology
Received: 2022/02/27 | Accepted: 2022/05/15 | ePublished: 2022/09/9

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