year 17, Issue 2 (March - April 2023)                   Iran J Med Microbiol 2023, 17(2): 167-175 | Back to browse issues page


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Salehi S, Nakhaei Moghadam M, Asgharian Rezaei Y, Najimi M, Yousefi E. Antibiotic Resistance Pattern and Prevalence of tetA, tetB, tetR, OXA-10 and OXA-48 Resistance Genes among Escherichia coli Isolates from Toilets in Mashhad Azad University (Iran) in 2020. Iran J Med Microbiol 2023; 17 (2) :167-175
URL: http://ijmm.ir/article-1-1626-en.html
1- Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
2- Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran , mahboobe_nak@yahoo.com
3- Department of Cell and Molecular Biology & Microbiology, faculty of biological science and Technology, University of Isfahan, Isfahan, Iran
Abstract:   (1726 Views)

Background and Aim: Escherichia coli is the normal flora of the human and animal intestine that sometimes causes pathogenesis within the intestine and urinary tract. The current study investigates resistance genes in Escherichia coli isolated from the toilets at the Islamic Azad University of Mashhad, Iran, and to perform genome sequencing in tetracycline-resistant strains.
Materials and Methods: This cross-sectional evaluation was carried out on 200 samples collected over three months. Samples were cultured and isolated using eosin methylene blue agar medium (EMB). E. coli Samples were identified and used to evaluate the resistance genes by polymerase chain reaction (PCR). The E. coli isolates were used for an Antibiogram test to identify resistant strains. For the statistical analysis of antibiotics pattern, SPSS-22 and the Kolmogorov-Smirnov test were used.
Results: Out of 200 samples, 41 isolates were identified as E. coli with the highest resistance rate to cefotaxime (74.60%) and the highest sensitivity rate to gentamicin (58.74%). Among the 20 isolates, 30%, 20%, 25% carried blaTEM-1, blaOXA-10 and blaOXA-48 genes, respectively. Among 38 tetracycline-resistant isolates, three isolates (7.89%) had tetA gene, and two (5.26%) had the tetR gene.
Conclusion: In this experiment, most of the isolates were identified as E. coli and were resistant to antibiotics. To control the spread of E. coli infectious disease and arbitrary use of antibiotics, it is recommended to conduct some educational programs and social activities with the aim of increasing health awareness.

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Type of Study: Original Research Article | Subject: Molecular Microbiology
Received: 2022/04/24 | Accepted: 2022/11/29 | ePublished: 2023/03/30

References
1. Belaynehe KM, Shin SW, Yoo HS. Interrelationship between tetracycline resistance determinants, phylogenetic group affiliation and carriage of class 1 integrons in commensal Escherichia coli isolates from cattle farms. BMC Vet Res. 2018;14(1):1-11. [DOI:10.1186/s12917-018-1661-3] [PMID] [PMCID]
2. Blum SE, Leitner G. Genotyping and virulence factors assessment of bovine mastitis Escherichia coli. Vet Microbiol. 2013;163(3-4):305-12. [DOI:10.1016/j.vetmic.2012.12.037] [PMID]
3. Kamrani Hemat N, Mirzaee M, Najar-peerayeh S. Prevalence of tetracycline resistance genes (tetA, tetB) and antibiotic resistance pattern in uropathogenic Escherichia coli isolates. New Cellular Mol Biotechnol j. 2017;7(25):9-18.
4. Olowe O, Idris O, Taiwo S. Prevalence of tet genes mediating tetracycline resistance in Escherichia coli clinical isolates in Osun State, Nigeria. Eur J Immunol Microbiol Immunol. 2013;3(2):135-40. [DOI:10.1556/EuJMI.3.2013.2.7] [PMID] [PMCID]
5. Taghinejad J, Barati B, Sadeghi A. A study of the drug resistance pattern of Group B Streptococcus isolated from urinary samples in the city of Salmas during the year 2015. New Cellular Mol Biotechnol j. 2018;8(30):79-84.
6. Ullmann A. Escherichia coli and the Emergence of Molecular Biology. EcoSal Plus. 2011;4(2). [DOI:10.1128/ecosalplus.1.1.2] [PMID]
7. Ruppé E. Épidémiologie des bêta-lactamases à spectre élargi: l'avènement des CTX-M. Antibiotiques. 2010;12(1):3-16. [DOI:10.1016/j.antib.2010.01.003]
8. Roshdi Maleki M, Taghinejad J, Momen Amir H. Drug susceptibility of E. coli strains isolated from patients with UTI by disc diffusion agar method in Salmas city of Iran. Health MED.1146.
9. Akya A, Gheisari H, Mohammadi G, Khodadoost M. Study of the pattern of plasmid and antibiotic resistance of Escherichia coli isolated from outpatients with urinary tract infection. Sci J Kurd Univ Med Sci. 2015;20(1):89-96.
10. Kadaei V, Rashki Ghalenoo Z. Identification and determination of tetracycline resistance genes in uropathogenic Escherichia coli isolates in Sistan. Vet Microbiol. 2019;2(1):1-9.
11. Tuckman M, Petersen PJ, Howe AY, Orlowski M, Mullen S, Chan K, et al. Occurrence of tetracycline resistance genes among Escherichia coli isolates from the phase 3 clinical trials for tigecycline. Antimicrob Agents Chemother. 2007;51(9):3205-11. [DOI:10.1128/AAC.00625-07] [PMID] [PMCID]
12. Forbes BA, Sahm DF, Weissfeld AS. Diagnostic microbiology: Mosby St Louis; 2007.
13. Satlin MJ, Lewis JS, Weinstein MP, Patel J, Humphries RM, Kahlmeter G, et al. Clinical and Laboratory Standards Institute and European Committee on Antimicrobial Susceptibility Testing position statements on polymyxin B and colistin clinical breakpoints. Clin Infect Dis. 2020;71(9):e523-e9. [DOI:10.1093/cid/ciaa121] [PMID]
14. Li X, Jensen BB, Højberg O, Noel SJ, Canibe N. Development of a species-specific TaqMan-MGB real-time PCR assay to quantify Olsenella scatoligenes in pigs offered a chicory root-based diet. AMB Express. 2018;8(1):1-13. [DOI:10.1186/s13568-018-0627-y] [PMID] [PMCID]
15. Moldagulova N, Ayupova A, Sembayeva D, Duambekov M, Khassenova E, Nagyzbekkyzy E, et al. Data on the isolation and identification of thermotolerant microorganisms from cow manure promising for organic waste processing. Data in brief. 2020;31:105761. [DOI:10.1016/j.dib.2020.105761] [PMID] [PMCID]
16. Hashemi AB, Nakhaei Moghaddam M, Forghanifard MM, Yousefi E. Detection of blaOXA-10 and blaOXA-48 Genes in Pseudomonas aeruginosa Clinical Isolates by Multiplex PCR. J Med Microbiol Infect Dis. 2021;9(3):142-7. [DOI:10.52547/JoMMID.9.3.142]
17. Xu G, An W, Wang H, Zhang X. Prevalence and characteristics of extended-spectrum β-lactamase genes in Escherichia coli isolated from piglets with post-weaning diarrhea in Heilongjiang province, China. Front Microbiol. 2015;6:1103. [DOI:10.3389/fmicb.2015.01103] [PMID] [PMCID]
18. Møller TS, Overgaard M, Nielsen SS, Bortolaia V, Sommer MO, Guardabassi L, et al. Relation between tetR and tetA expression in tetracycline resistant Escherichia coli. BMC Microbiol. 2016;16(1):1-8. [DOI:10.1186/s12866-016-0649-z] [PMID] [PMCID]
19. Moghadam M N, MM F. Antibiotic susceptibility and multi-drug resistance of escherichia coli isolates producing CTX-M and TEM type beta-lactamases in Mashhad, Iran, in 2010. 2012.
20. Naji-Hamad M, Nakhaei-Moghaddam M. Detection of Tetracycline-Resistant Genes by Multiplex Polymerase Chain Reaction in Clinical Isolates of Klebsiella Pneumonia. J Isfahan Med Sch. 2021;39(647):815-20.
21. Chaudhary M, Payasi A. Prevalence, genotyping of Escherichia coli and Pseudomonas aeruginosa clinical isolates for Oxacillinase resistance and mapping susceptibility behaviour. J Microb Biochem Technol. 2014;6(2):63-7. [DOI:10.4172/1948-5948.1000123]
22. Bokaian M, Shahraki S, Raeisi J, Mohammadzadeh Rostami F. Prevalence of OXA-2 and OXA-10 Type ESBL among Acinetobacter Strains Isolated from Patients of Zahedan (South Eastern Iran). Med J Mashhad Univ Med Sci. 2016;59(1):26-34.
23. Hujer Kristine M, Hujer Andrea M, Hulten Edward A, Bajaksouzian S, Adams Jennifer M, Donskey Curtis J, et al. Analysis of Antibiotic Resistance Genes in Multidrug-Resistant Acinetobacter sp. Isolates from Military and Civilian Patients Treated at the Walter Reed Army Medical Center. Antimicrob Agents Chemother. 2006;50(12):4114-23. [DOI:10.1128/AAC.00778-06] [PMID] [PMCID]
24. Amiri M, Farzin H, Jamshidian-Mojaver M. Phenotypic and Genotypic Study of Antibiotic Resistance among Escherichia coli Isolates from Human Urinary Infection Cases in Bojnord Province. Avicenna J Clin Med. 2019;26(3):173-80. [DOI:10.29252/ajcm.26.3.173]
25. Mousavi P, Zaker Bostan Abad S, Mirnejad R. Detection of TEM beta-lactamase Gene in Escherichia Coli Isolated from Urinary Tract Infection of referred to the clinic boarding Robat Karim to the molecular method. N Cell Mol Biotechnol J. 2018;8(30):85-94.
26. Maurya AP, Dhar D, Basumatary MK, Paul D, Ingti B, Choudhury D, et al. Expansion of highly stable bla OXA-10 β-lactamase family within diverse host range among nosocomial isolates of Gram-negative bacilli within a tertiary referral hospital of Northeast India. BMC Res Notes. 2017;10(1):1-6. [DOI:10.1186/s13104-017-2467-2] [PMID] [PMCID]
27. Adefisoye MA, Okoh AI. Identification and antimicrobial resistance prevalence of pathogenic Escherichia coli strains from treated wastewater effluents in Eastern Cape, South Africa. MicrobiologyOpen. 2016;5(1):143-51. [DOI:10.1002/mbo3.319] [PMID] [PMCID]
28. Malik I, Elhag K, Gustaw K. Characterisation of extended-spectrum β-lactamases among multidrug resistant Enterobacteriaceae from Sudan. J Pure Appl Microbiol. 2019;13(1):8. [DOI:10.22207/JPAM.13.1.06]
29. Boroumand M, Naghmachi M, Ghatee MA. Detection of phylogenetic groups and drug resistance genes of Escherichia coli causing urinary tract infection in southwest Iran. Jundishapur J Microbiol. 2021;14(2). [DOI:10.5812/jjm.112547]
30. Badi S, Cremonesi P, Abbassi MS, Ibrahim C, Snoussi M, Bignoli G, et al. Antibiotic resistance phenotypes and virulence-associated genes in Escherichia coli isolated from animals and animal food products in Tunisia. FEMS Microbiol Lett. 2018;365(10):fny088. [DOI:10.1093/femsle/fny088] [PMID]
31. Gurung S, Kafle S, Dhungel B, Adhikari N, Shrestha UT, Adhikari B, et al. Detection of OXA-48 gene in carbapenem-resistant Escherichia coli and Klebsiella pneumoniae from urine samples. Infect Drug Resist. 2020;13:2311. [DOI:10.2147/IDR.S259967] [PMID] [PMCID]

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