Prevalence of the per, tem, veb, shv genes in Acinetobacter baumannii Isolated from Educational Hospital of Zahedan, Iran

Ordoni, Ramin; Amini, Yousef; Ranayi, Morad Ali; Avestan, Zoleikha; Mohagheghi-Fard, Amir Hossein; Zandhaghighi, Mehdi; Bameri, Zakaria; Kord, Ebrahim; Shahraki-Zahedani, Shahram; Dehvari, Aslam

doi:10.30699/ijmm.16.6.566

year 16, Issue 6 (November - December 2022) Iran J Med Microbiol 2022, 16(6): 566-572 | Back to browse issues page

‎ 10.30699/ijmm.16.6.566

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Ordoni R, Amini Y, Ranayi M A, Avestan Z, Mohagheghi-Fard A H, Zandhaghighi M, et al . Prevalence of the per, tem, veb, shv genes in Acinetobacter baumannii Isolated from Educational Hospital of Zahedan, Iran. Iran J Med Microbiol 2022; 16 (6) :566-572
URL: http://ijmm.ir/article-1-1585-en.html

Prevalence of the per, tem, veb, shv genes in Acinetobacter baumannii Isolated from Educational Hospital of Zahedan, Iran

Ramin Ordoni¹

, Yousef Amini¹

, Morad Ali Ranayi¹

, Zoleikha Avestan²

, Amir Hossein Mohagheghi-Fard¹

, Mehdi Zandhaghighi¹

, Zakaria Bameri¹

, Ebrahim Kord¹

, Shahram Shahraki-Zahedani

³, Aslam Dehvari¹

1- Department of Microbiology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran
2- Community Nursing Research Center, Zahedan University of Medical Sciences, Zahedan, Iran
3- Department of Microbiology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran , shahram17ir@yahoo.com

Abstract: (1706 Views)

Background and Aim: Acinetobacter baumannii is one of the most important pathogens found in ICUs as it is responsible for nosocomial infection with a high mortality rate and a rising level of resistance to most antibiotics. One of the main mechanisms of resistance is the production of extended-spectrum beta-lactamase (ESBL) genes such as veb, per, tem, and shv. The current study aimed to determine the antibiotic resistance pattern and the frequency of per, tem, veb, and shv genes in A. baumannii strains isolated from Zahedan, Iran.
Materials and Methods: The current study was conducted on 150 strains of A. baumannii isolated from different clinical samples from January to September 2018. The antibiotic resistance pattern was determined by the disk diffusion method for 18 antibiotics and the minimum inhibitory concentration for colistin. Later, the bacterial genome was extracted, and PCR detected ESBL genes.
Results: Out of the 150 studied isolates, 141 were A. baumannii and only nine isolates were A. nosocomialis. A. baumannii strains were strongly resistant to many selected antibiotics such as CAZ (99.3%), CTX (97.9), PTZ (97.2%), SXT (97.2%), IMI (97.2%), and LEV (96.5%); while this value was low for a few antibiotics including MN (69.5%), DXT (52.5%), SAM (21.3), and TN (16.7). The eb, per, tem, and shv genes were detected in 20 (13.3%), 15 (10%), 23 (15.4%), and 11 (7.3%) isolates, respectively.
Conclusion: The isolates were highly resistant to ceftazidime, ceftriaxone, piperacillin-tazobactam, cotrimoxazole, imipenem, and levofloxacin; in addition, the frequency of per, veb, tem, and genes was lower in the current study than those reported in other regions.

Keywords: Acinetobacter baumannii, Extended-spectrum beta-lactamase, Antibiotic resistance

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Type of Study: Original Research Article | Subject: Medical Bacteriology
Received: 2021/12/12 | Accepted: 2022/07/7 | ePublished: 2022/09/9

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