year 16, Issue 2 (March - April 2022)                   Iran J Med Microbiol 2022, 16(2): 134-140 | Back to browse issues page


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Ghasemshahi S, Ahmadpor M, Booskabadi A, Rezaei H, Poopak B, Hakemi-Vala M. A Six-Month Survey of the Frequency of Extensively Drug-resistant Gram-Negative Bacteria by VITEK 2 System in 2020. Iran J Med Microbiol 2022; 16 (2) :134-140
URL: http://ijmm.ir/article-1-1432-en.html
1- Department of Microbiology, Payvand Clinical and Specialty Laboratory, Tehran, Iran
2- Head of Payvand Clinical and Specialty Laboratory, Tehran, Iran
3- Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences (SBMU), Tehran, Iran , mojdeh_hakemi@yahoo.com
Abstract:   (1640 Views)

Background and Objective: Increasing of resistant bacteria is a major concern globally. The emergence of XDR gram-negative bacteria is a more serious problem due to treatment limitations. This study aimed to evaluate the frequency of extensively drug-resistant (XDR) gram-negative bacterial isolates in different clinical samples from Payvand Clinical and Specialty Laboratory, Tehran, Iran, for 6 months by VITEK 2 system.
Materials and Methods: During March 2020-September 2020, different clinical samples were collected from patients referred to Payvand Clinical and Specialty Laboratory. Bacterial identification and antimicrobial susceptibility test (AST) were performed applying an automated VITEK 2 system. The frequency of identified bacteria, their resistance to common antibiotics and also XDR bacteria were calculated and reported, respectively.
Results: Overall, 4125 urine specimens, 34 sputum samples, and 1 tracheal aspirate tube were submitted to Payvand Laboratory during 6 months. Of them, 486 urine, 32 sputum, and a tracheal aspirate tube samples were culture positive. Gram-negative isolated bacteria were included in this study. Based on AST, 63.3% of the isolated Klebsiella pneumoniae, 100% of Pseudomonas aeruginosa, and all Acinetobacter baumannii isolates were susceptible to amikacin and colistin. Totally, 31 XDR gram-negative bacteria, including: K. pneumonia (ssp. pneumonia (n=20), and ozaenae (n=2), Escherichia coli (n=3), P. aeruginosa (n=5), and A. baumannii (n=1) were identified from 18 urine samples, 12 sputum specimens, and a tracheal aspirate tube. 
Conclusion: The rate of XDR bacteria was high in the investigated laboratory in this study. Therefore, accurate screening and antimicrobial stewardship is recommended in different medical centers of Iran.

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Type of Study: Original Research Article | Subject: Antibiotic Resistance
Received: 2021/08/14 | Accepted: 2022/01/8 | ePublished: 2022/02/10

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