Inhibitory Effect of Supernatant and Lysate of Saccharomyces cerevisiae on Expression of exoA Gene of Pseudomonas aeruginosa

Ghorbani, Zahra; Koupaei, Maryam; Saderi, Horieh; Amin Marashi, Seyed Mahmoud; DehghanZadeh, Zahra; Owlia, Parviz

doi:10.30699/ijmm.17.3.288

year 17, Issue 3 (May - June 2023) Iran J Med Microbiol 2023, 17(3): 288-293 | Back to browse issues page

‎ 10.30699/ijmm.17.3.288

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Ghorbani Z, Koupaei M, Saderi H, Amin Marashi S M, DehghanZadeh Z, Owlia P. Inhibitory Effect of Supernatant and Lysate of Saccharomyces cerevisiae on Expression of exoA Gene of Pseudomonas aeruginosa. Iran J Med Microbiol 2023; 17 (3) :288-293
URL: http://ijmm.ir/article-1-1402-en.html

Inhibitory Effect of Supernatant and Lysate of Saccharomyces cerevisiae on Expression of exoA Gene of Pseudomonas aeruginosa

Zahra Ghorbani¹

, Maryam Koupaei²

, Horieh Saderi²

, Seyed Mahmoud Amin Marashi³

, Zahra DehghanZadeh¹

, Parviz Owlia

⁴

1- Department of Microbiology, School of Medicine, Shahed University, Tehran, Iran
2- Department of Microbiology and Immunology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran
3- Department of Microbiology, Qazvin University of Medical Sciences, Qazvin, Iran
4- Molecular Microbiology Research Center (MMRC), Shahed University, Tehran, Iran , powlia@gmail.com

Abstract: (916 Views)

Background and Aim: Pseudomonas aeruginosa is an important ubiquitous and especially common pathogen in the hospital. Exotoxin A that encoded by exoA gene has a role in pathogenesis of this bacterium. Today, probiotics are widely used in the treatment and prevention of diseases. The present study aimed to study the Saccharomyces cerevisiae S3 effect on the expression of exoA gene.
Materials and Methods: S. cerevisiae S3 supernatant and lysate were prepared. Subminimum inhibitory concentrations (sub-MIC) of extracts were used to P. aeruginosa PAO1. The level of exoA expression was measured with real-time PCR method.
Results: Lysate extract had a reducing effect on toxin gene expression, but unlike lysate, supernatant had an increasing effect on gene expression.
Conclusion: We demonstrated that S. cerevisiae S3 had an inhibitory effect on Exotoxin A virulence factor of P. aeruginosa. We suggest doing more experiments on the effect of S. cerevisiae on other virulence factors of P. aeruginosa and pathogens.

Keywords: Exotoxin A, Probiotics, Pseudomonas aeruginosa, Saccharomyces cerevisiae

Full-Text [PDF 579 kb] (305 Downloads) | | Full-Text (HTML) (179 Views)

Type of Study: Original Research Article | Subject: Medical Bacteriology
Received: 2022/10/25 | Accepted: 2023/03/28 | ePublished: 2023/06/26

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