year 15, Issue 1 (January - February 2021)                   Iran J Med Microbiol 2021, 15(1): 107-120 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ghods Zadeh M, Moradi Bidhendi S, Ashrafi F. Comparative Study of Garlic Extract Effect on the Expression of Genes Involved in Quorum Sensing in Pseudomonas aeruginosa and the Antibiotic Tobramycin. Iran J Med Microbiol 2021; 15 (1) :107-120
URL: http://ijmm.ir/article-1-1201-en.html
1- Master of Microbiology, Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran
2- Associate Professor, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran , s.moradibidhendi@yahoo.com
3- Assistant Professor, Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran
Abstract:   (4446 Views)

Background: The expression of most genes involved in pathogenesis in Pseudomonas aeruginosa is controlled and regulated by a gene system called quorum sensing (QS). The purpose of this study was to investigate the quorum quenching of garlic extract in preventing the expression of genes involved in QS system of P. aeruginosa.
Methods: In the present cross-sectional study, 12 P. aeruginosa strains were collected from burn wounds, cultured on special media and then confirmed by differential tests. The PCR test was performed to detect the lasI and lasR genes. The expression level of lasI gene was assessed in the presence of garlic extract and tobramycin antibiotic by real-time PCR, and the active ingredient of garlic extract was analyzed by HPLC.
Results: Examination of 12 strains cultured on special and differential media showed that all 12 strains were P. aeruginosa. The PCR results indicated that all strains had 100% lasI and lasR genes. The real-time PCR results also revealed that the garlic extract was able to reduce the expression level of lasI gene in P. aeruginosa. It was found that tobramycin has a greater ability to reduce the expression level of this gene compared with garlic extract.
Conclusion: This study showed that the garlic extract could reduce the expression of lasI gene in P. aeruginosa isolates, and that the active ingredients of this extract could be used to treat infections as an alternative to antibiotic therapy.

Full-Text [PDF 1141 kb]   (1673 Downloads) |   |   Full-Text (HTML)  (1291 Views)  
Type of Study: Original Research Article | Subject: Antimicrobial Substances
Received: 2020/08/9 | Accepted: 2020/10/13 | ePublished: 2021/01/10

References
1. Waters CM, Bassler BL. Quorum sensing: cell-to-cell communication in bacteria. Annu Rev Cell Dev Biol. 2005;21:319-46. [DOI:10.1146/annurev.cellbio.21.012704.131001] [PMID]
2. Papenfort K, Bassler BL. Quorum sensing signal-response systems in Gram-negative bacteria. Nature Reviews Microbiology. 2016;14(9):576. [DOI:10.1038/nrmicro.2016.89] [PMID] [PMCID]
3. Solano C, Echeverz M, Lasa I. Biofilm dispersion and quorum sensing. Current opinion in microbiology. 2014;18:96-104. [DOI:10.1016/j.mib.2014.02.008] [PMID]
4. Trappetti C, Oggioni MR. Biofilm Formation Under In Vitro Conditions. Streptococcus Pneumoniae: Elsevier; 2015. p. 245-55. [DOI:10.1016/B978-0-12-410530-0.00013-2]
5. Oliver A, Mulet X, López-Causapé C, Juan C. The increasing threat of Pseudomonas aeruginosa high-risk clones. Drug Resistance Updates. 2015;21:41-59. [DOI:10.1016/j.drup.2015.08.002] [PMID]
6. Brackman G, Coenye T. Quorum sensing inhibitors as anti-biofilm agents. Current pharmaceutical design. 2015;21(1):5-11. [DOI:10.2174/1381612820666140905114627] [PMID]
7. Truchado P, Larrosa M, Castro-Ibáñez I, Allende A. Plant food extracts and phytochemicals: their role as quorum sensing inhibitors. Trends in Food Science & Technology. 2015;43(2):189-204. [DOI:10.1016/j.tifs.2015.02.009]
8. Bergey DH, Hendricks D, Holt JG, Sneath PH. Bergey's Manual of systematic bacteriology. Vol. 2: Williams & Wilkins; 1984.
9. Bratu S, Gupta J, Quale J. Expression of the las and rhl quorum-sensing systems in clinical isolates of Pseudomonas aeruginosa does not correlate with efflux pump expression or antimicrobial resistance. Journal of Antimicrobial Chemotherapy. 2006;58(6):1250-3. [DOI:10.1093/jac/dkl407] [PMID]
10. O'Loughlin CT, Miller LC, Siryaporn A, Drescher K, Semmelhack MF, Bassler BL. A quorum-sensing inhibitor blocks Pseudomonas aeruginosa virulence and biofilm formation. Proceedings of the National Academy of Sciences. 2013;110(44):17981-6. [DOI:10.1073/pnas.1316981110] [PMID] [PMCID]
11. Zhou Y, Kong Y, Kundu S, Cirillo JD, Liang H. Antibacterial activities of gold and silver nanoparticles against Escherichia coli and bacillus Calmette-Guérin. Journal of nanobiotechnology. 2012;10(1):19. [DOI:10.1186/1477-3155-10-19] [PMID] [PMCID]
12. Narusaka M, Shiraishi T, Iwabuchi M, Narusaka Y. rpoD gene expression as an indicator of bacterial pathogens in host plants. Journal of General Plant Pathology. 2011;77(2):75-80. [DOI:10.1007/s10327-011-0298-x]
13. Lewis K. Platforms for antibiotic discovery. Nature reviews Drug discovery. 2013;12(5):371-87. [DOI:10.1038/nrd3975] [PMID]
14. Reiter J, Hübbers AM, Albrecht F, Leichert LIO, Slusarenko AJ. Allicin, a natural antimicrobial defence substance from garlic, inhibits DNA gyrase activity in bacteria. International Journal of Medical Microbiology. 2020;310(1):151359. [DOI:10.1016/j.ijmm.2019.151359] [PMID]
15. Dwivedi VP, Bhattacharya D, Singh M, Bhaskar A, Kumar S, Fatima S, et al. Allicin enhances antimicrobial activity of macrophages during Mycobacterium tuberculosis infection. Journal of ethnopharmacology. 2019;243:111634. [DOI:10.1016/j.jep.2018.12.008] [PMID]
16. Choo S, Chin VK, Wong EH, Madhavan P, Tay ST, Yong PVC, et al. antimicrobial properties of allicin used alone or in combination with other medications. Folia Microbiologica. 2020:1-15. [DOI:10.1007/s12223-020-00786-5] [PMID]
17. Schuster M, Lostroh CP, Ogi T, Greenberg EP. Identification, timing, and signal specificity of Pseudomonas aeruginosa quorum-controlled genes: a transcriptome analysis. Journal of bacteriology. 2003;185(7):2066-79. [DOI:10.1128/JB.185.7.2066-2079.2003] [PMID] [PMCID]
18. Smith RS, Harris SG, Phipps R, Iglewski B. The Pseudomonas aeruginosa quorum-sensing molecule N-(3-oxododecanoyl) homoserine lactone contributes to virulence and induces inflammation in vivo. Journal of bacteriology. 2002;184(4):1132-9. [DOI:10.1128/jb.184.4.1132-1139.2002] [PMID] [PMCID]
19. Bazargani MM, Rohloff J. Antibiofilm activity of essential oils and plant extracts against Staphylococcus aureus and Escherichia coli biofilms. Food control. 2016;61:156-64. [DOI:10.1016/j.foodcont.2015.09.036]
20. Bjarnsholt T, Jensen PØ, Rasmussen TB, Christophersen L, Calum H, Hentzer M, et al. Garlic blocks quorum sensing and promotes rapid clearing of pulmonary Pseudomonas aeruginosa infections. Microbiology. 2005;151(12):3873-80. [DOI:10.1099/mic.0.27955-0] [PMID]
21. Fulghesu L, Giallorenzo C, Savoia D. Evaluation of different compounds as quorum sensing inhibitors in Pseudomonas aeruginosa. Journal of chemotherapy. 2007;19(4):388-90. [DOI:10.1179/joc.2007.19.4.388] [PMID]
22. Rasmussen TB, Bjarnsholt T, Skindersoe ME, Hentzer M, Kristoffersen P, Köte M, et al. Screening for quorum-sensing inhibitors (QSI) by use of a novel genetic system, the QSI selector. Journal of bacteriology. 2005;187(5):1799-814. [DOI:10.1128/JB.187.5.1799-1814.2005] [PMID] [PMCID]
23. Bodini S, Manfredini S, Epp M, Valentini S, Santori F. Quorum sensing inhibition activity of garlic extract and p‐coumaric acid. Letters in applied microbiology. 2009;49(5):551-5. [DOI:10.1111/j.1472-765X.2009.02704.x] [PMID]
24. Harjai K, Kumar R, Singh S. Garlic blocks quorum sensing and attenuates the virulence of Pseudomonas aeruginosa. FEMS Immunology & Medical Microbiology. 2010;58(2):161-8. [DOI:10.1111/j.1574-695X.2009.00614.x] [PMID]
25. Annapoorani A, Umamageswaran V, Parameswari R, Pandian SK, Ravi AV. Computational discovery of putative quorum sensing inhibitors against LasR and RhlR receptor proteins of Pseudomonas aeruginosa. Journal of computer-aided molecular design. 2012;26(9):1067-77. [DOI:10.1007/s10822-012-9599-1] [PMID]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Iranian Journal of Medical Microbiology

Designed & Developed by : Yektaweb | Publisher: Farname Inc