year 13, Issue 3 (July - August 2019)
Iran J Med Microbiol 2019, 13(3): 194-209 |
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Rahimi N, Honarmand Jahromy S, Zare Karizi S. Evaluation of Antibiotic Resistance Pattern of Meropenem and Piperacillin- Tazobactam in Multi Drug Resistant Acinetobacter baumannii Isolates by Flow Cytometry Method. Iran J Med Microbiol 2019; 13 (3) :194-209
URL: http://ijmm.ir/article-1-955-en.html
URL: http://ijmm.ir/article-1-955-en.html
1- Department of Microbiology, School of Biological Sciences, Varamin- Pishva Branch, Islamic Azad University, Varamin, Iran
2- Department of Microbiology, School of Biological Sciences, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran ,sahar_hj2@yahoo.com
3- Department of Genetics, School of Biological Sciences, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran
2- Department of Microbiology, School of Biological Sciences, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran ,
3- Department of Genetics, School of Biological Sciences, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran
Abstract: (6655 Views)
Background and Aims: Flow cytometry is a rapid method that can analyze thousands of cells per second and can be used for determination of microbial populations and determination of bacterial antimicrobial susceptibility. In this study antibiotic resistance pattern of Acinetobacter baumannii isolates by flow cytometer was evaluated.
Materials and Methods: 55 isolates of Acinetobacter baumannii were isolated from clinical specimen of patients and were identified by biochemical tests. Antibiotic resistance patterns were studied by disc diffusion method and MDR strains were selected. MIC of Meropenem and Piperacillin were determined. Also antibiotic resistance pattern of isolates was determined by coloring with Rhodamine-123 and flow cytometry.
Results: 98% of isolates were MDR. The MIC ranges for maropenem were 8 - 256 μg/mL and for piperacillin were 128-1024 μg/mL. By flow cytometry it was demonstrated that at concentrations of 8, 4 and 2 μg/mL of meropenem, only 1.96%, 1.44% and 0.59%, of cells were killed respectively. At concentrations of 64,128 and 16 μg/mL of piperacillin, 13.8%, 11.3% and 5.9%of cells were killed respectively. Reducing the number of living bacteria was observed with increasing concentrations of both antibiotics.
Conclusion: The similarity between the results of flow cytometry and both agar and broth antibacterial susceptibility methods showed flow cytometry as a reliable and rapid test that can be used for this purpose.
Materials and Methods: 55 isolates of Acinetobacter baumannii were isolated from clinical specimen of patients and were identified by biochemical tests. Antibiotic resistance patterns were studied by disc diffusion method and MDR strains were selected. MIC of Meropenem and Piperacillin were determined. Also antibiotic resistance pattern of isolates was determined by coloring with Rhodamine-123 and flow cytometry.
Results: 98% of isolates were MDR. The MIC ranges for maropenem were 8 - 256 μg/mL and for piperacillin were 128-1024 μg/mL. By flow cytometry it was demonstrated that at concentrations of 8, 4 and 2 μg/mL of meropenem, only 1.96%, 1.44% and 0.59%, of cells were killed respectively. At concentrations of 64,128 and 16 μg/mL of piperacillin, 13.8%, 11.3% and 5.9%of cells were killed respectively. Reducing the number of living bacteria was observed with increasing concentrations of both antibiotics.
Conclusion: The similarity between the results of flow cytometry and both agar and broth antibacterial susceptibility methods showed flow cytometry as a reliable and rapid test that can be used for this purpose.
Type of Study: Original Research Article |
Subject:
Antibiotic Resistance
Received: 2019/08/9 | Accepted: 2019/11/22 | ePublished: 2019/11/22
Received: 2019/08/9 | Accepted: 2019/11/22 | ePublished: 2019/11/22
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