year 12, Issue 6 (January - February 2019)                   Iran J Med Microbiol 2019, 12(6): 399-408 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Faraji Kafshgari S, Maghsoudlou Y, Khomeyri M, Kashiri M, Babaei A. In Vitro Biocontrol of Escherichia coli Through the Immobilization of its Specific Lytic Bacteriophage on Cellulose Acetate Biodegradable Film. Iran J Med Microbiol. 2019; 12 (6) :399-408
URL: http://ijmm.ir/article-1-896-en.html
1- Department of Food Science and Technology, Faculty of Food Industries, University of Agricultural Sciences and Natural Resources, Gorgan, Iran
2- Department of Food Science and Technology, Faculty of Food Industries, University of Agricultural Sciences and Natural Resources, Gorgan, Iran , y.maghsoudlou@gau.ac.ir
3- Department of Biology, Faculty of Basic Sciences, Malayer University, Malayer, Iran
Abstract:   (1096 Views)
Background and Aims: Bacteriophages are mandatory bacterial parasites that are harmless to human and animal, which are used by dipping or spraying in food as natural antimicrobial agents. The use of these methods leads to wasting or trapping of phage in food, but its immobilization on the polymer surface facilitates the contact of phage with the host cell at the food surface. Therefore, the aim of this study was to immobilize the lytic phage of Escherichia coli on the cellulose acetate film and investigate of its antimicrobial effect.
Materials and Methods: Escherichia.coli bacteria was incubated at 37°C for 24 hours, and the antimicrobial effect of phages was evaluated through plaque forming. The cellulose acetate film was prepared by casting, then modificated by plasma, and immersed in a suspension of phage (1010 PFU/ml) and incubated at 37 °C for 24 hours with slow shaking, then the number of immobilized phages was estimated. To confirm the immobilization, FESEM was done. The antimicrobial effect of the active film was evaluated by disk diffusion and the release rate and antimicrobial activity of immobilized phages were investigated in 14 days.
Results: Phages formed clear plaques against E.coli. Modification of film by plasma resulted in uniform immobilization (108 PFU/ml) that FESEM revealed it. The active film (with zone diameter 12 mm) showed stronger antimicrobial effect than the antibiotic ampicillin (positive control sample with zone diameter 8 mm). 11 days after the immobilization, the number of immobilized phages decreased from 108 to 106 (PFU/ml) and released from the film surface, afterwards did not release. The antimicrobial activity of active film was decreased due to the absence of host bacteria continuously in 15 days, so that the host bacteria population increased from 3 to 5.3 LOG CFU/ml.
 Conclusions: In spite of reducing the antimicrobial activity of cellulose acetate active film over the time, due to the presence of host bacteria at food surface and its high potential in destroying the host bacteria, it can be used to increase of food safety in food packaging.
 
Full-Text [PDF 1115 kb]   (181 Downloads)    
Type of Study: Original | Subject: Food Microbiology
Received: 2018/12/8 | Accepted: 2019/02/13

References
1. Siragusa GR, Dickson JS. Inhibition of Listeria monocytogenes on beef tissue by application of organic acids immobilized in a calcium alginate gel. Journal of Food Science. 1992; 57(2): 293-6. [DOI:10.1111/j.1365-2621.1992.tb05479.x]
2. Burt S. Essential oils: their antibacterial properties and potential applications in foods-a review. Int J Food Microbiol. 2004; 94(3): 223-53. [DOI:10.1016/j.ijfoodmicro.2004.03.022] [PMID]
3. Labuza TP, Breene WM. Applications of Active Packaging for Improvement of Shelf-life and Nutritional Quality of Fresh and Extended Shelf-life Foods 1. J Food Process Preserv. 1989; 13(43): 9-252. [DOI:10.1111/j.1745-4549.1989.tb00090.x]
4. Doyle MP, Schoeni JL. Isolation Escherichia coli O157: H7 from retail fresh meats and poultry. Appl Environ Microbiol. 1987; 53(10): 2394-6.
5. Horrocks SM, Anderson RC, Nisbet DJ, Ricke SC. Incidence and ecology of Campylobacter jejuni and coli in animals. Anaerobe. 2009; 15(1-2): 18-25. [DOI:10.1016/j.anaerobe.2008.09.001] [PMID]
6. Molla B, Mesfin A, Alemayehu D. Multiple antimicrobial-resistant Salmonella serotypes isolated from chicken carcass and giblets in Debre Zeit and Addis Ababa, Ethiopia. Ethiop J Health Dev. 2003; 17(2):131-49. [DOI:10.4314/ejhd.v17i2.9854]
7. Meshkani M, Mortazavi A, Pourfallah Z. Antimicrobial and physical properties of a chickpea protein isolate-based film containing essential oil of thyme using response surface methodology. Iranian Journal of Nutrition Sciences & Food Technology. 2013; 8(1): 93-104.
8. Sharma M, Goodridge L. Bacteriophages: back to the future. Food Technology. 2013; 67(5): 46-55.
9. Hagens S, Loessner MJ. Bacteriophage for biocontrol of foodborne pathogens: calculations and considerations. Curr Pharm Biotechnol. 2010; 11(1): 58-68. [DOI:10.2174/138920110790725429] [PMID]
10. Anany H, Chen W, Pelton R, Griffiths MW. Biocontrol of Listeria monocytogenes and Escherichia coli O157:H7 in meat by using phages immobilized on modified cellulose membranes. Appl Environ Microbiol. 2011; 77(18): 6379- 87. [DOI:10.1128/AEM.05493-11] [PMID] [PMCID]
11. Goddard JM, Hotchkiss JH. Polymer surface modification for the attachment of bioactive compounds. Prog polym sci. 2007; 32(7): 698-725. [DOI:10.1016/j.progpolymsci.2007.04.002]
12. Fridman A, Friedman G. Plasma Medicine. First Edition. 2013: 448-82. [DOI:10.1002/9781118437704]
13. Sun W, Brovko L, Griffiths M. Use of bioluminescent Salmonella for assessing the efficiency of constructed phage-based biosorbent. J Ind Microbiol Biotechnol. 2001; 27(2): 126-8. [DOI:10.1038/sj.jim.7000198] [PMID]
14. Cerqueira DA, Rodrigues Filho G, Carvalho RD, Valente AJ. 1H-NMR characterization of cellulose acetate obtained from sugarcane bagasse. Polímeros. 2010; 20(2): 85-91. [DOI:10.1590/S0104-14282010005000017]
15. Zare L, Shenagari M, Mirzaei MKH, Mojtahedi A. Isolation of lytic phages against pathogenic E.coli isolated from diabtic ulcers. Iran J Med Microbiol 2018; 11 (2): 34-41.
16. Aghaei Z, Emadzadeh B, Ghorani B, Kadkhodaei R. Investigation of the Hallucoremic behavior of cellulose acetate film containing a bromothymole blue. New food Technol. 2016; 4(14): 55-66. [In Persion]. http://jift.irost.ir/article_390_f6ecda9f4ee216eabf5b0c678bf19607.pdf.
17. Wang C, Sauvageau D, Elias AL. Immobilization of Active Bacteriophages on Polyhydroxyalkanoate Surfaces. ACS Appl Mater Interfaces. 2015; 1-42.
18. Ranjbar M, Sharifiyan A, Shabani Sh, Amin Afshar M. Antimicrobial effect of Garlic extract on Staphylococcus aureus and Escherichia coli bacteria in a cook ready chicken to meal model. Food Technology and Nutrition . 2014; 11 (4): 57-68.
19. Vonasek E, Le P, Nitin N. Encapsulation of bacteriophages in whey protein films for extended storage and release. Food Hydrocoll. 2014; 37: 7-13. [DOI:10.1016/j.foodhyd.2013.09.017]
20. Tolba M, Minikh O, Brovko LY, Evoy S, Griffiths MW. Oriented immobilization of bacteriophages for biosensor applications. Appl Environ Microbiol. 2010; 76(2): 528-35. [DOI:10.1128/AEM.02294-09] [PMID] [PMCID]
21. Gouvêa DM, Mendonça RC, Soto ML, Cruz RS. Acetate cellulose film with bacteriophages for potential antimicrobial use in food packaging. LWT-Food Science and Technology. 2015; 63(1): 85-91. [DOI:10.1016/j.lwt.2015.03.014]
22. Sohar J, Griffiths M. Immobilization of bacteriophages for the control and detection of food-borne pathogens. A Thesis presented to The University of Guelph. 2014; Guelph, Ontario, Canada.
23. Soltan Dallal MM, Imeni SM, Nikkhahi F, Rajabi Z, Salas SP. Isolation of E. Coli Bacteriophage from Raw Sewage and Comparing Its Antibacterial Effect with Ceftriaxone Antibiotic. Int J Adv Biotechnol Res. 2016; 7(3): 385-91.
24. Singh V, Jain P, Dahiya S. Isolation and characterization of bacteriophage from waste water against E.coli, a food born pathogen. Asian J Microbiol Biotechnol Environ Sci. 2016; (1): 163-70.
25. Griffiths MW. Phage-based methods for the detection of bacterial pathogens. In Bacteriophages in the control of food-and waterborne pathogens. American Society of Microbiology. 2010 : 31-59. [DOI:10.1128/9781555816629.ch3] [PMID] [PMCID]
26. Geyter ND, Morent R, Leys C. Surface modification of a polyester non-woven with a dielectric barrier discharge in air at medium pressure. Surface & Coatings Technology. 2006; 201 (6): 2460-6. [DOI:10.1016/j.surfcoat.2006.04.004]
27. Darki N, Navab Safa N, Ranaei Siadat SO, Jahanfar M, Ghasemi S, Ghomi H. Modification of chitosan/PEO nanofiber surface by dielectric barrier discharge plasma for bio applications. 15th surface engineering national conference, Materials and Energy research center. 2014; October 22.

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


© 2019 All Rights Reserved | Iranian Journal of Medical Microbiology

Designed & Developed by : Yektaweb