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:: year 12, Issue 3 (July - August 2018) ::
Iran J Med Microbiol 2018, 12(3): 0-0 Back to browse issues page
Investigating the Protective Effect of Egg Yolk Immunoglobulin (IgY) Against Aflatoxin on Blood Parameters, Ileum Morphometry and Hepatocytes’ Histopathology of Broiler Chickens Fed Aflatoxin B1
Ali Nakhaei 1, Nazar Afzali 2, Seyyed Javad Hosseini Vashan 2, Mohammad Amir Karimi Torshizi 3
1- Department of Animal Sciences, Faculty of Agriculture, University of Birjand, Birjand, Iran , nafzali@birjand.ac.ir
2- Department of Animal Sciences, Faculty of Agriculture, University of Birjand, Birjand, Iran
3- Department of Animal Sciences, Faculty of Agriculture, University of Tarbiyat modares, Tehran, Iran
Abstract:   (500 Views)
Background and Aims: The aim of this study was to evaluate the protective effect of specific egg yolk immunoglobulin (IgY) against aflatoxin on reducing the defects of aflatoxin by adding to the drinking water of 192 day-old Ross 308 broiler chickens from 1 to 42 days.
Materials and Methods: The experiment was based on a completely randomized design with 6 treatments, 4 replications, and 8 observations (chicks). First, immunized egg yolk against aflatoxin B1 was produced by laying hens injected aflatoxin-BSA conjugate in order to add to broilers' drinking water. The experimental treatments were: 1) control (without any additives); 2) ration contaminated with 1 mg kg-1 aflatoxin B1 (negative control treatment); 3) negative control + 0.5% (V/V) immunized yolk against AFB1, 4) negative control + 0.5% (V/V) unimmunized yolk against AFB1; 5) negative control + 1% (V/V) immunized yolk against AFB1 and 6) negative control + 1% (V/V) unimmunized yolk against AFB1.
Results: Using aflatoxin contaminated diet significantly increased serum cholesterol, and decreased serum total protein and albumin concentration (P<0.05). Also, histopathologic lesions observed in the liver. Adding 1 % (V/V) of immunized egg yolk to the drinking water (treatment 5) reduced serum cholesterol and increased total protein concentration compared to treatment 2 (P<0.05). The length and width of the villi and the villi surface area of chicks receiving treatment 5 were higher than treatment 2 (P<0.05). Liver tissue in chicken receiving treatment 3 and 5, was almost normal and few changes observed in hepatocytes.
Conclusion: The results indicate that specific IgY against AFB1 can be effective in reducing the defects of experimental aflatoxicosis as a detoxification agent.
Keywords: Aflatoxin B1, Blood parameters, Specific immunoglobulin
     
Type of Study: Original | Subject: Medical Mycology
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Nakhaei A, Afzali N, Hosseini Vashan S J, Karimi Torshizi M A. Investigating the Protective Effect of Egg Yolk Immunoglobulin (IgY) Against Aflatoxin on Blood Parameters, Ileum Morphometry and Hepatocytes’ Histopathology of Broiler Chickens Fed Aflatoxin B1. Iran J Med Microbiol. 2018; 12 (3)
URL: http://ijmm.ir/article-1-848-en.html


year 12, Issue 3 (July - August 2018) Back to browse issues page
مجله میکروب شناسی پزشکی ایران Iranian Journal of Medical Microbiology
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