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Iran J Med Microbiol 2018, 12(4): 248-259 Back to browse issues page
Protective Efficacy of a Divalent Candidate Vaccine Consisting of Type A Flagellin and Pilin Against Dermal Pseudomonas aeruginosa Infection in a Murine Burn Model
Farhad B. Hashemi 1, Bahador Behrouz 2
1- Department of Microbiology, Tehran University of Medical Sciences, Tehran, Iran , farhadb.hashemi@gmail.com
2- Department of Microbiology, Tehran University of Medical Sciences, Tehran, Iran
Abstract:   (615 Views)
Background and Aims: Pseudomonas aeruginosa is an opportunistic pathogen that causes serious infections and high mortality among burn patients. The aim of this study is to evaluate the protective effects of a candidate divalent vaccine containing type A flagellin and pilin of P. aeruginosa in a burn wound mouse model.
Materials and Methods: Recombinant flagellin A and pilin proteins were generated by expressing fliC and pilA genes (cloned in pET-28a and pET-22b vectors, respectively) in E. coli BL-21. Groups of mice were immunized by injection of 10 µg of either flagellin A and pilin, or flagellin A, or pilin. Specific IgG titer was measured by ELISA. The functional activity of antibodies was evaluated by opsonophagocytosis assay. The protective effects of the vaccine were evaluated by measuring mortality and bacterial load in mice.
Results: Immunization with flagellin A and pilin mixture significantly increased the specific IgG antibody titer as well as opsonophagocytosis compared to monovalent antigens (P<0.05). Immunization with flagellin A and pilin mixture significantly reduced the bacterial load, and increased the survival of mice challenged with P. aeruginosa, as compared to the monovalent antigens (P<0.05).
 Conclusions: Immunization with flagellin A and pilin mixture provides effective protection against P. aeruginosa wound infection in burned mice. Reduced bacterial load, and high survival rates among immunized mice suggest that flagellin A and pilin candidate vaccine shows therapeutic potential against P. aeruginosa infections among burn patients.
Keywords: Pseudomonas aeruginosa, Flagellin a, Pilin, Opsonophagocytosis
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Type of Study: Original | Subject: Medical Bacteriology
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B. Hashemi F, Behrouz B. Protective Efficacy of a Divalent Candidate Vaccine Consisting of Type A Flagellin and Pilin Against Dermal Pseudomonas aeruginosa Infection in a Murine Burn Model. Iran J Med Microbiol. 2018; 12 (4) :248-259
URL: http://ijmm.ir/article-1-819-en.html


year 12, Issue 4 (September - October 2018) Back to browse issues page
مجله میکروب شناسی پزشکی ایران Iranian Journal of Medical Microbiology
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