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Iran J Med Microbiol 2018, 12(1): 0-0 Back to browse issues page
Isolation of ASR7 Actinomycete Isolated from S12 Demospongia Marine Sponge and Study of its Antibacterial Activity
Razieh Pordel 1, Soheila Matroodi Dr 2, Isac Zamani Dr1
1- Assistant Professor, Department of Marine Biology, Khorramshahr Marine Science and Technology University, Khorramshahr, Iran
2- Assistant Professor, Department of Marine Biology, Khorramshahr Marine Science and Technology University, Khorramshahr, Iran , s.matroodi@kmsu.ac.ir
Abstract:   (38 Views)
Background and Aims: Marine actinomycetes are gram-positive bacteria that sometimes are free, saprophytic or plants and animals- associated, including marine sponges. More than 75% of antibiotics and antimicrobial compounds are produced by actinomycetes. In recent years, due to the need for new drugs, marine microorganisms have been considered as new sources of potential production of significant metabolites. The purpose of this study is isolation and identification of marine sponge-associated actinomycete and investigation of its antibacterial activity.
Materials and Methods: The actinomycete was isolated from the marine Sponge collected from the depths of Bushehr coast waters and screened for antibacterial activity on pathogenic microorganisms of Escherichia coli، Bacillus cereus، Klebsiella sp., Salmonella sp. and Proteus sp. using a Disk Diffusion Method. For molecular identification, genomic DNA was first extracted from isolate and then, the16S rDNA gene was amplified by PCR and Sequenced. The results were analyzed using bioinformatics programs, Bioedit and MEGA6.
Results: In this study, based on phylogeny studies, it was determined that the isolate was belonged to the genus streptomyces, and biochemical studies showed that all tests except catalase and gram were negative and antibacterial activity study showed significant activity against three pathogenic bacteria, especially Salmonella sp.
Conclusion: The results showed that depths of the Bushehr coast waters have marine sponge associated actinomycetes, which are a source of secondary metabolites with biological activity.

 
Keywords: Actinomycete, Marine Sponge, Antibacterial Activity, Pathogenic Bacteria, 16S rDNA
     
Type of Study: Original | Subject: Microbial Biotechnology
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Pordel R, Matroodi S, Zamani I. Isolation of ASR7 Actinomycete Isolated from S12 Demospongia Marine Sponge and Study of its Antibacterial Activity. Iran J Med Microbiol. 2018; 12 (1)
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year 12, Issue 1 (March - April 2018) Back to browse issues page
مجله میکروب شناسی پزشکی ایران Iranian Journal of Medical Microbiology
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