Identification and assessment of Salmonella typhimurium, infantis and enteritidis serotypes in clinical samples from medical centers of Kerman province

Moghadam, Abolfazl; Nazarian, Shahram; Amani, Jafar

year 11, Issue 2 (May - June 2017) Iran J Med Microbiol 2017, 11(2): 1-8 | Back to browse issues page

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Moghadam A, Nazarian S, Amani J. Identification and assessment of Salmonella typhimurium, infantis and enteritidis serotypes in clinical samples from medical centers of Kerman province. Iran J Med Microbiol 2017; 11 (2) :1-8
URL: http://ijmm.ir/article-1-620-en.html

Identification and assessment of Salmonella typhimurium, infantis and enteritidis serotypes in clinical samples from medical centers of Kerman province

Abolfazl Moghadam¹

, Shahram Nazarian

², Jafar Amani³

1- Department of Biology, College of Science, University of Tehran, Tehran, Iran
2- Department of Biology, Faculty of Science, Imam Hossein University, Tehran, Iran , nazarian56@gmail.com
3- Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

Abstract: (14497 Views)

Background and Aim: Salmonellosis is one of the important infectious diseases and can be as spread disease between humans and animals that make it essential for identification and detection of Salmonella. Housekeeping genes are typically important genes which are necessary for maintenance and survival of basic cells and can be considered as a gene diagnostic screening bacterial agents. The aim of this study was analysis of Flic, Sdf1 and FljB, housekeeping genes for screening of typhimurium, infantis and enteritidis serovars isolated in Kerman’s hospitals.

Materials and Methods: In a descriptive study from February 2015 to August 2015, 132Salmonella specimens were taken from patients with acute gastroenteritis referred to different medical centers and hospitals in Kerman. The specimens were transferred to microbiology laboratory for identification of Salmonella with serological and bacteriological standard methods. DNA of Salmonella genus strains were extracted by CTAB and, specific primers of housekeeping genes of Salmonella genus (invA) and typhimurium(fliC), infantis(fljB) and, enteritidis(sdfI) serotypes are used in PCR test.

Results: Using PCR technique, the presence of Salmonella genus were confirmed by amplification of invA gene in 130 out of 132 specimens which are identified as a Salmonella by microbiological and biochemical methods (98%). Also results indicating the prevalence of 19% in infantis, 22% in Salmonella typhimurium and 32% in Salmonella enteritidis.

Conclusions: Results showed that the prevalence of Salmonella enteritidis is more than any other serotypes in this region, but as the global statistics, the prevalence of typhimurium and Infantis are increasing.

Keywords: Housekeeping genes, PCR, Salmonella typhimurium, Salmonella infantis, Salmonella enteritidis

Full-Text [PDF 604 kb] (6479 Downloads)

Type of Study: Original Research Article | Subject: Medical Bacteriology
Received: 2016/09/14 | Accepted: 2016/12/26 | ePublished: 2017/06/7

References

1. Su LH, Chiu CH. Salmonella: clinical importance and evolution of nomenclature. Chang Gung Med J 2007; 30(3):210–9. [PubMed]

2. Soltan Dallal MM, Sharifi Yazdi M, Mirzaei N, Kalantar E. Prevalence of Salmonella spp. in Packed and Unpacked Red Meat and Chicken in South of Tehran. Jundishapur J Microbiol 2014;7(4):562–8.[in persian] [PubMed]

3. Nosrat S, Sabokbar A, Dezfoolian M, Tabarraie B, Fallah F. Prevalence of Salmonella enteritidis, typhi and typhimurium from food products in Mofid hospital. Res Med 2012;36(1):43–8. [in persian] [Article]

4. Silver N, Best S, Jiang J, Thein S. Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR. BMC Mol Biol 2006;7(1):33. [PubMed]

5. Miller Ma, Sentz J, Rabaa Ma, Mintz Ed. Global epidemiology of infections due to Shigella, Salmonella serotype Typhi, and enterotoxigenic Escherichia coli. Epidemiol Infect 2008;136(04): 433-5. [PubMed]

6. Gonera E. Salmonellosis in 1997. Przegl Epidemiol 1999; 53(1-2):83–91.

7. Rahn K, De Grandis SA, Clarke RC, McEwen SA, Galán JE, Ginocchio C, et al. Amplification of an invA gene sequence of Salmonella typhimurium by polymerase chain reaction as a specific method of detection of Salmonella. Mol Cell Probes 1992; 6(4):271–9. [PubMed]

8. Malorny B, Hoorfar J, Bunge C, Helmuth R. Multicenter validation of the analytical accuracy of Salmonella PCR: towards an international standard. Appl Environ Microbiol 2003; 69(1):290–6. [Article]

9. Darwin KH, Miller VL. Molecular basis of the interaction of Salmonella with the intestinal mucosa. Clin Microbiol Rev 1999; 12(3):405–28. [PubMed]

10. Oliveira SD, Santos LR, Schuch DMT, Silva AB, Salle CTP, Canal CW. Detection and identification of salmonellas from poultry-related samples by PCR. Vet Microbiol 2002; 87(1):25–35. [PubMed]

11. Agron PG, Walker RL, Kinde H, Sawyer SJ, Hayes DC, Wollard J, et al. Identification by subtractive hybridization of sequences specific for Salmonella enterica serovar enteritidis. Appl Environ Microbiol 2001; 67(11):4984–91. [PubMed]

12. Kardos G, Farkas T, Antal M, Nógrády N, Kiss I. Novel PCR assay for identification of Salmonella enterica serovar Infantis. Lett Appl Microbiol 2007; 45(4):421–5. [PubMed]

13. Eshraghi S, Soltan Dalall M, Fardsanei F, Zahraii Salehi T, Ranjbar R, Nikmanesh B. Salmonella enteritidis and antibiotic resistance patterns: A study on 1950 children with diarrhea. Tehran Univ Med J 2010; 67(12):876–82. [in persian] [Article]

14. Wang TY, Wang L, Zhang JH, Dong WH. A simplified universal genomic DNA extraction protocol suitable for PCR. Genet Mol Res 2011; 10(1):519–25. [PubMed]

15. Jamshidi A, Bassami M., Afshari-Nic S. Identification of Salmonella spp. and Salmonella typhimurium by a multiplex PCR-based assay from poultry carcasses in Mashhad- Iran. IntJVetRes 2009; 3(1):43–8. [Article]

16. Lim Y-H, Hirose K, Izumiya H, Arakawa E, Takahashi H, Terajima J, et al. Multiplex polymerase chain reaction assay for selective detection of Salmonella enterica serovar typhimurium. Jpn J Infect Dis 2003; 56(4):151–5. [PubMed]

17. de Freitas CG, Santana AP, da Silva PHC, Gonçalves VSP, Barros M de AF, Torres FAG, et al. PCR multiplex for detection of Salmonella Enteritidis, typhi and typhimurium and occurrence in poultry meat. Int J Food Microbiol 2010; 139(1-2):15–22. [PubMed]

18. Lin JS, Tsen HY. Development and use of polymerase chain reaction for the specific detection of Salmonella typhimurium in stool and food samples. J Food Prot 1999; 62(10):1103–10.

19. Adak GK. Trends in indigenous foodborne disease and deaths, England and Wales: 1992 to 2000. Gut. 2002; 51(6):832–41. [PubMed]

20. Pan TM, Liu YJ. Identification of Salmonella enteritidis isolates by polymerase chain reaction and multiplex polymerase chain reaction. J Microbiol Immunol Infect 2002; 35(3):147–51. [PubMed]

21. Jafari RA, Ghorbanpour M, Jaideri A. An Investigation into Salmonella Infection Status in Backyard Chickens in Iran. Int J Poult Sci 2007; 6(3):227–9. [in persian] [Article]

22. Mehrabyan S, Rafiee Tabatabaei R, Hajiyan A. Study of type and drug resistance in salmonella isolated from foods. J Sci 2001; 1:193–9. [in persian]

23. Jinu M, Agarwal RK, Sailo B, Wani MA, Kumar A, Dhama K, et al. Comparison of PCR and Conventional Cultural Method for Detection of Salmonella from Poultry Blood and Faeces. Asian J Anim Vet Adv 2014; 9(11):690–701. [Article]

24. Soria MC, Soria MA, Bueno DJ. Comparison of 2 culture methods and PCR assays for Salmonella detection in poultry feces. Poult Sci. 2012; 91(3):616–26. [PubMed]

25. Soltan Dallal M, Rahimi Forushani A, Sadigh Maroufi S, Sharifi Yazdi K. The comparison of PCR technique and API-20E kit with the conventional biomedical methods for the identification of Salmonella species in laboratory. mljgoums 2011; 5(2):20–7. [in persian] [Article]

26. Report of the Task Force on Zoonoses Data Collection on the Analysis of the baseline survey on the prevalence of Salmonella in broiler flocks of Gallus gallus, in the EU, 2005-2006 - Part A: Salmonella prevalence estimates. EFSA J 2007; 5(3):98.

27. Gal-Mor O, Valinsky L, Weinberger M, Guy S, Jaffe J, Schorr YI, et al. Multidrug-resistant Salmonella enterica serovar Infantis, Israel. Emerg Infect Dis 2010; 16(11):1754–7. [PubMed]

28. Ranjbar R, Sarshar M, Sadeghifard N, AWT_TAG. Characterization of Genetic Diversity among Clinical Strains of Salmonella enterica Serovar Infantis by Ribotyping Method. ZUMS J 2012; 20(81):75-84. [in persian] [Article]

29. Rahmani M, Peighambari SM, Svendsen CA, Cavaco LM, Agersø Y, Hendriksen RS. Molecular clonality and antimicrobial resistance in Salmonella enterica serovars Enteritidis and Infantis from broilers in three Northern regions of Iran. BMC Vet Res 2013; 9(1):66 [PubMed]

30. Ward MP, Alinovi CA, Couëtil LL, Wu CC. Evaluation of a PCR to detect Salmonella in fecal samples of horses admitted to a veterinary teaching hospital. J Vet Diagn Invest 2005; 17(2):118–23. [PubMed]

31. Trkov M, Majerikova I, Jerasek B, Stefanovicova A, Rijpens N, Kuchta T. Detection of Salmonella in food over 30 h using enrichment and polymerase chain reaction. Food Microbiol 1999; 16:393–9. [Article]