year 4, Issue 1 And 2 (9-2010)
Iran J Med Microbiol 2010, 4(1 And 2): 17-25 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Nateghian A, Arjmandi K, Vosough P, Karimi A, Behzad A, Navidnia M. Detrmination of Vancomycin resistant enterococci (VRE) carriage , molecular epidemiology and the related risk factors in children with ALL in Ali-asghar children hospital and Mahak hospital,Tehran,Iran . Iran J Med Microbiol 2010; 4 (1 and 2) :17-25
URL: http://ijmm.ir/article-1-55-en.html
URL: http://ijmm.ir/article-1-55-en.html
Alireza Nateghian1 
, Khadijeh Arjmandi2 , Parvaneh Vosough3 , Abdollah Karimi4 , Azita Behzad5 , Masomeh Navidnia6
, Khadijeh Arjmandi2 , Parvaneh Vosough3 , Abdollah Karimi4 , Azita Behzad5 , Masomeh Navidnia6
1- Department of Pediatric infectious diseases, Ali-asghar children hospital, Tehran University of Medical Sciences
2- Department of Pediatric Hematology-oncology, Ali-asghar children hospital, Tehran University of Medical Sciences
3- Department of Pediatric Hematology-oncology, Mahak hospital, Tehran University of Medical Sciences
4- Department of Pediatric infectious diseases, Pediatric Infectious diseases research center,Mofid hospital, Shahid Beheshti University of Medical Sciences
5- Pediatrician, Ali-asghar children hospital, Tehran University of Medical Sciences
6- Department of Microbiology, Pediatric Infectious diseases research center,Mofid hospital, Shahid Beheshti University of Medical Sciences
2- Department of Pediatric Hematology-oncology, Ali-asghar children hospital, Tehran University of Medical Sciences
3- Department of Pediatric Hematology-oncology, Mahak hospital, Tehran University of Medical Sciences
4- Department of Pediatric infectious diseases, Pediatric Infectious diseases research center,Mofid hospital, Shahid Beheshti University of Medical Sciences
5- Pediatrician, Ali-asghar children hospital, Tehran University of Medical Sciences
6- Department of Microbiology, Pediatric Infectious diseases research center,Mofid hospital, Shahid Beheshti University of Medical Sciences
Abstract: (17339 Views)
Bachground and Objectives: vancomycin resistant Enterococcus (VRE) in immune deficient patient is a significant factor for increase in morbidity and mortality. The aim of this study was to determine VRE carriage rate , its molecular epidemiology and to identify risk factors for acquisition of VRE in children with Acute lymphoblastic leukemia (ALL) in 2007-2008.
Material &Methods: In a cross-sectional study, stool samples of children with ALL in Aliasghar and Mahak hospitals were randomly analyzed for detection of VRE . Enterococci were identified by conventional bacteriologic methods and vancomycin resistance was detected by disk diffusion method and E-test. MIC of vancomycin wase determined by macrodilution method. VanA and VanB genes characterization was performed by a multiplex polymerase chain reaction (PCR), which also confirmed the identification. Questionnaires of patient was done to determine risk factors. Data were analyzed by STT , Mann whithney new , Chi-sqare , Fisher exact , Anova and Kruskal-Wallis tests.
Results: In the present study, of 130 child with ALL admitted to our hematology/oncology an infection units, 33(34.7%) of the 130 patient were colonized with VRE(25.3% of total cases) and remainder of patients were colonized with vancomycin-sensitive strains (VSE), serving as controls. 26 isolates(78.8%) of VRE detected with VanA phenotype and seven isolates(21.2%) with VanB phenotype. In the univariate analysis history of ICU admission(P=0.03)was significantly associated with Enterococcus colonization and had a trend with VRE colonization. age (P=0,007)was significantly associated with VRE phenotype .There was no significant relationship between gender, duration of disease ,number of admission , another disease, history of severe neutropenia among one month ago and history of antibiotic use in three month ago and VRE colonization.
Conclusion: VRE prevalence is high in children with ALL in Iran ,indicating a prompt intervention strategy in the control of VRE particularly in PICU.
Material &Methods: In a cross-sectional study, stool samples of children with ALL in Aliasghar and Mahak hospitals were randomly analyzed for detection of VRE . Enterococci were identified by conventional bacteriologic methods and vancomycin resistance was detected by disk diffusion method and E-test. MIC of vancomycin wase determined by macrodilution method. VanA and VanB genes characterization was performed by a multiplex polymerase chain reaction (PCR), which also confirmed the identification. Questionnaires of patient was done to determine risk factors. Data were analyzed by STT , Mann whithney new , Chi-sqare , Fisher exact , Anova and Kruskal-Wallis tests.
Results: In the present study, of 130 child with ALL admitted to our hematology/oncology an infection units, 33(34.7%) of the 130 patient were colonized with VRE(25.3% of total cases) and remainder of patients were colonized with vancomycin-sensitive strains (VSE), serving as controls. 26 isolates(78.8%) of VRE detected with VanA phenotype and seven isolates(21.2%) with VanB phenotype. In the univariate analysis history of ICU admission(P=0.03)was significantly associated with Enterococcus colonization and had a trend with VRE colonization. age (P=0,007)was significantly associated with VRE phenotype .There was no significant relationship between gender, duration of disease ,number of admission , another disease, history of severe neutropenia among one month ago and history of antibiotic use in three month ago and VRE colonization.
Conclusion: VRE prevalence is high in children with ALL in Iran ,indicating a prompt intervention strategy in the control of VRE particularly in PICU.
Type of Study: Original Research Article |
Subject:
Antibiotic Resistance
Received: 2013/11/10 | Accepted: 2013/11/10 | ePublished: 2013/11/10
Received: 2013/11/10 | Accepted: 2013/11/10 | ePublished: 2013/11/10
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Copyright Policy
Iranian Journal of Medical Microbiology by Farname is licensed under CC BY-NC 4.0
