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Iran J Med Microbiol 2018, 12(3): 0-0 Back to browse issues page
Identification and Characterization of Staphylococcus Aureus Methicillin and Vancomycin Resistance From Patients in Sari and Ghaemshahr Injuries and Burn Hospitals in 2015
Mohammad Movagharnezhad 1, Mohammad reza Khataminezhad 2
1- Department of Microbiology, Faculty of Biology, Islamic Azad University, Tonekabon, Iran. , mohammadmovagharnezhad@yahoo.com
2- Department of Microbiology, Faculty of Biology, Islamic Azad University, Tonekabon, Iran.
Abstract:   (479 Views)

Background and Aims: Staphylococcus aureus is the most common and important nosocomial pathogens and due to potential virulence and increasing resistance to anti-microbial medicines, they become one of the most important health problem through worldwide. So the aim of this study was identification and characterization of S. aureus resistant to Methicillin and Vancomycin from patients hospitalized in Razi hospital of Ghaemshahr and Shahid Zare of Sari and characteristics antibiotics susceptibility pattern in 2015.
Methods: In this cross sectional study 134 samples from patients in infectious and burn hospital isolated and transferred to the research laboratory. Samples cultured in blood agar and incubated for 24 hours at 37 ° C. Gram stain and standard microbiology and biochemical test, including catalase, coagulase, Dnase, mannitol and resistance to polymyxin and sensitivity were examined Novobiocin tested for identification and characterization. Antibiotic susceptibility test was performed by disk diffusion method. Identification was done by PCR and results confirmed by sequencing method.
Results:A total of 134 samples, 100 samples were positive for S. aureus that the number of 51 samples of methicillin-resistant and 2 samples were resistant to Vancomycin with vanA and vanB resistance gene.
Conclusion: Expression of new resistance factor in nosocomial infection is one of the major challenges in treating these infections. 25/37% of the samples, weren’t S. aureus. While resistance to Methicillin significantly more than Vancomycin, we reported, bout 27% of the samples were resistant to Vancomycin.

Keywords: Staphylococcus aureus, resistance, methicillin, Vancomycin.
     
Type of Study: Original | Subject: Nosocomial infections
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movagharnezhad M, khataminezhad M R. Identification and Characterization of Staphylococcus Aureus Methicillin and Vancomycin Resistance From Patients in Sari and Ghaemshahr Injuries and Burn Hospitals in 2015. Iran J Med Microbiol. 2018; 12 (3)
URL: http://ijmm.ir/article-1-525-en.html


year 12, Issue 3 (July - August 2018) Back to browse issues page
مجله میکروب شناسی پزشکی ایران Iranian Journal of Medical Microbiology
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