[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: year 12, Issue 3 (July - August 2018) ::
Iran J Med Microbiol 2018, 12(3): 160-168 Back to browse issues page
Identification and Characterization of Staphylococcus Aureus Methicillin and Vancomycin Resistance From Patients in Sari and Ghaemshahr Injuries and Burn Hospitals in 2015
Mohammad Movagharnezhad 1, Mohammad reza Khataminezhad2
1- Department of Microbiology, Faculty of Biology, Islamic Azad University, Tonekabon, Iran. , mohammadmovagharnezhad@yahoo.com
2- Department of Microbiology, Faculty of Biology, Islamic Azad University, Tonekabon, Iran.
Abstract:   (1756 Views)

Background and Aims: Staphylococcus aureus is the most common and important nosocomial pathogens and due to potential virulence and increasing resistance to anti-microbial medicines, they become one of the most important health problems through worldwide. So the aim of this study was identification and characterization of S. aureus resistant to Methicillin and Vancomycin from patients hospitalized in Razi hospital of Ghaemshahr and Shahid Zare of Sari and characteristics antibiotics susceptibility pattern in 2015.
Materials and Methods: In this cross-sectional descriptive study, 134 strains of Staphylococcus aureus from hospitalized patients in infectious diseases and burns were collected randomly from the hospital laboratory and transferred to the research laboratory. The specimens were incubated in Blood Agar medium for 24 hours at 37 ° C. The colonies were examined for morphology, biochemical properties, resistance to polymixin and sensitivity to Novobiocin. For isolates, antibiotic test was performed using disk diffusion method and PCR detection was performed. PCR results were approved for sequencing.
Results: 100 out of 134 samples were positive for S. aureus; 51 samples were methicillin-resistant and 2 samples were resistant to all of the antibiotics and Vancomycin with vanA and vanB resistance gene.
Conclusions: Determination of new resistance factor in nosocomial infection is one of the major challenges in treating these infections. 25.37% of the samples, weren’t S. aureus. This study showed 51% prevalence of methicillin-resistance.

Keywords: Staphylococcus aureus, Resistance, Methicillin, Vancomycin
Full-Text [PDF 652 kb]   (274 Downloads)    
Type of Study: Original | Subject: Nosocomial infections
References
1. Yoshida K, Ekstedt RD. Relation of mucoid growth of Staphylococcus aureus to clumping factor reaction, morphology in serum-soft agar, and virulence. J Bacteriol. 1968;96(4):902-8.
2. Owens WE, Nickerson SC. Morphologic study of Staphylococcus aureus L-form, reverting, and intermediate colonies in situ. J Clin Microbiol. 1989;27(6):1382-6.
3. Tong SY, Davis JS, Eichenberger E, Holland TL, Fowler VG, Jr. Staphylococcus aureus infections: epidemiology, pathophysiology, clinical manifestations, and management. Clin Microbiol Rev. 2015;28(3):603-61.
4. Holland TL, Arnold C, Fowler VG, Jr. Clinical management of Staphylococcus aureus bacteremia: a review. JAMA. 2014;312(13):1330-41.
5. Vaez H, Ghazi Saeidi K, Moradi A, Tabaraei A, Khodabakhshi B, Bazouri M, et al. Antibiotic resistance pattern of methicillin resistant Staphylococcus aureus isolated from Health-educational centers of Gorgan, Iran, 2008-2009. Iranian Journal of Medical Microbiology. 2010;3(4):31-6.
6. Davis JS, Sud A, O'Sullivan MV, Robinson JO, Ferguson PE, Foo H, et al. Combination of Vancomycin and beta-Lactam Therapy for Methicillin-Resistant Staphylococcus aureus Bacteremia: A Pilot Multicenter Randomized Controlled Trial. Clin Infect Dis. 2016;62(2):173-80.
7. Leiva Pelaez O, Stojanov M, Zayas Tamayo AM, Barreras Garcia G, Gonzalez Aleman M, Martinez Ceballos L, et al. Molecular epidemiology of methicillin-resistant Staphylococcus aureus from 4 Cuban hospitals. Diagn Microbiol Infect Dis. 2015;81(1):1-3.
8. Luini M, Cremonesi P, Magro G, Bianchini V, Minozzi G, Castiglioni B, et al. Methicillin-resistant Staphylococcus aureus (MRSA) is associated with low within-herd prevalence of intra-mammary infections in dairy cows: Genotyping of isolates. Vet Microbiol. 2015;178(3-4):270-4.
9. Kahanov L, Kim YK, Eberman L, Dannelly K, Kaur H, Ramalinga A. Staphylococcus aureus and community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) in and around therapeutic whirlpools in college athletic training rooms. J Athl Train. 2015;50(4):432-7.
10. Kumari VH, Babu AR, Srinivas D, Siddaiah N, Somanna S. Methicillin-Resistant Staphylococcus aureus central nervous system infections--Analysis and outcome. Br J Neurosurg. 2015;29(3):413-8.
11. Holmes NE, Tong SY, Davis JS, van Hal SJ. Treatment of methicillin-resistant Staphylococcus aureus: vancomycin and beyond. Semin Respir Crit Care Med. 2015;36(1):17-30.
12. Inomata S, Yano H, Tokuda K, Kanamori H, Endo S, Ishizawa C, et al. Microbiological and molecular epidemiological analyses of community-associated methicillin-resistant Staphylococcus aureus at a tertiary care hospital in Japan. J Infect Chemother. 2015;21(10):729-36.
13. Cazares-Dominguez V, Cruz-Cordova A, Ochoa SA, Escalona G, Arellano-Galindo J, Rodriguez-Leviz A, et al. Vancomycin tolerant, methicillin-resistant Staphylococcus aureus reveals the effects of vancomycin on cell wall thickening. PLoS One. 2015;10(3):e0118791.
14. Soong G, Paulino F, Wachtel S, Parker D, Wickersham M, Zhang D, et al. Methicillin-resistant Staphylococcus aureus adaptation to human keratinocytes. MBio. 2015;6(2):12-4
15. Ahmad MK, Asrar A. Prevalence of methicillin resistant Staphylococcus aureus in pyogenic community and hospital acquired skin and soft tissues infections. J Pak Med Assoc. 2014;64(8):892-5.
16. Kim CJ, Kim HB, Oh MD, Kim Y, Kim A, Oh SH, et al. The burden of nosocomial staphylococcus aureus bloodstream infection in South Korea: a prospective hospital-based nationwide study. BMC Infect Dis. 2014;14:590.
17. Grammatico-Guillon L, Thiolet JM, Bernillon P, Coignard B, Khoshnood B, Desenclos JC. Relationship between the prevalence of methicillin-resistant Staphylococcus aureus infection and indicators of nosocomial infection control measures: a population-based study in French hospitals. Infect Control Hosp Epidemiol. 2009;30(9):861-9.
18. Sancak B, Ercis S, Menemenlioglu D, Colakoglu S, Hascelik G. Methicillin-resistant Staphylococcus aureus heterogeneously resistant to vancomycin in a Turkish university hospital. J Antimicrob Chemother. 2005;56(3):519-23.
19. Teyhoo M, Mobin H, Mozafari N A, Moadab S R, Sedigh Bayan KH, Mones Rast SH. The Prevalence of Toxin Shock Syndrome oxin ( TSST-1) Producing Clinical Isolates of Staphylococcus aureus StrainsIsolated from Shohada Hospital in Tabriz, Iran. Medical Laboratory Journal. 2011;5(1):38-44.
20. Darabi N, Habibollahi H, Shahbabian K. Molecular Epidemiology of Staphylococcus aureus Isolated from patients and personnel in Army hospital. Ann Mil Health Sci Res. 2010;8(3):193-9.
21. Afrough P, Pourmand MR, Zeinalinia N, Yousefi M, Abdossamadi Z, Bagherzadeh Yazdchi S. Molecular Typing of Clinical and Nasal Carriage Isolates of Staphylococcus Aureus by spa Gene Patterns. Journal of Mazandaran University of Medical Sciences. 2012;22(94):28-34.
22. heydari f, Irannejad S, Amirmozaffari N, Yusefzadeh T, Karimi S. The Study of antibiotic resistance pattern of methicillin resistant Staphylococcus aureus isolated from urinary tract infection of outpatients in Salmas (West Azerbaijan Province) during 2010-12. Journal of Microbial World. 2014:-.
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA code



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Movagharnezhad M, khataminezhad M R. Identification and Characterization of Staphylococcus Aureus Methicillin and Vancomycin Resistance From Patients in Sari and Ghaemshahr Injuries and Burn Hospitals in 2015. Iran J Med Microbiol. 2018; 12 (3) :160-168
URL: http://ijmm.ir/article-1-525-en.html


year 12, Issue 3 (July - August 2018) Back to browse issues page
مجله میکروب شناسی پزشکی ایران Iranian Journal of Medical Microbiology
Persian site map - English site map - Created in 0.05 seconds with 32 queries by YEKTAWEB 3897