Molecular diagnosis of Coxiella burnetii in milk based on Plasmid and Transposon Genes

Javadi, Zahra; Ownagh, Abdolghaffar

doi:10.30699/ijmm.17.5.550

year 17, Issue 5 (September - October 2023) Iran J Med Microbiol 2023, 17(5): 550-558 | Back to browse issues page

‎ 10.30699/ijmm.17.5.550

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Javadi Z, Ownagh A. Molecular diagnosis of Coxiella burnetii in milk based on Plasmid and Transposon Genes. Iran J Med Microbiol 2023; 17 (5) :550-558
URL: http://ijmm.ir/article-1-2023-en.html

Molecular diagnosis of Coxiella burnetii in milk based on Plasmid and Transposon Genes

Zahra Javadi¹

, Abdolghaffar Ownagh

1- Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, West Azerbaijan, Iran
2- Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, West Azerbaijan, Iran , a.ownagh@urmia.ac.ir

Abstract: (477 Views)

Background and Aim: All Coxiella burnetii isolates carry one of four large, conserved, autonomously replicating plasmids or a plasmid-like chromosomally integrated sequence.
Materials and Methods: In Sulaimani City, Iraq, from September 2020 to September 2021, 200 positive nasopharyngeal samples were collected, and 17 known variants with the S gene were randomly selected for whole RdRp, E, and N gene sequencing. To facilitate sequencing, six primer sets were designed for the RdRp gene (RdRp1, RdRp2, RdRp3), two for the N gene (N1, N2), and one for the E gene.
Results: In total, out of 400 milk samples collected from cow, buffalo, sheep, and goats based on the IS1111 gene, 62 (15.5%), (95% CI: 12.3%–19.4%) samples were positive for C. burnetii. Out of 62 positive samples, 16 (25.8%), (95% CI: 16.6%–37.9%) samples contained QpH1 plasmid gene and 5 (8%), (95% CI: 3.5%–17.5%) samples contained QpRS plasmid gene. Also, there were 7 (11.3%), (95% CI: 5.6%–21.5%) positive samples for QpDG and 5 (11.3%), (95% CI: 3.5%–17.5%) positive to QpDV gene. The Phylogenetic analysis of plasmid sequences showed that all obtained sequences have 100% similarity. A phylogenetic tree constructed based on neighbor-joining analysis of partial genes revealed that 20 sequenced isolates were closely clustered together showing 99.9% similarity which can be considered identical and also revealed the 100% similarly of these sequences with more sequences in the gene bank from different sources.
Conclusion: Our results indicated that nested PCR has high sensitivity in detecting plasmids.

Keywords: Milk, Q fever, Coxiella burnetii, Plasmid, Transposon genes

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Type of Study: Original Research Article | Subject: Zoonoses Research
Received: 2023/04/25 | Accepted: 2023/08/17 | ePublished: 2023/11/29

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