Optimization of Production Conditions for Bioactive Polysaccharides from Fomes fomentarius and Investigation of Antibacterial and Antitumor Activities

Alvandi, Hale; Hatamian-Zarmi, Ashrafalsadat; Ebrahimi Hosseinzadeh, Bahman; Mokhtari-Hosseini, Zahra-Beagom

doi:10.30699/ijmm.14.6.596

year 14, Issue 6 (November - December 2020) Iran J Med Microbiol 2020, 14(6): 596-611 | Back to browse issues page

‎ 10.30699/ijmm.14.6.596

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Alvandi H, Hatamian-Zarmi A, Ebrahimi Hosseinzadeh B, Mokhtari-Hosseini Z. Optimization of Production Conditions for Bioactive Polysaccharides from Fomes fomentarius and Investigation of Antibacterial and Antitumor Activities. Iran J Med Microbiol 2020; 14 (6) :596-611
URL: http://ijmm.ir/article-1-1208-en.html

Optimization of Production Conditions for Bioactive Polysaccharides from Fomes fomentarius and Investigation of Antibacterial and Antitumor Activities

Hale Alvandi¹

, Ashrafalsadat Hatamian-Zarmi

², Bahman Ebrahimi Hosseinzadeh¹

, Zahra-Beagom Mokhtari-Hosseini³

1- Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran
2- Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran , hatamian_a@ut.ac.ir
3- Department of Chemical Engineering, Faculty of Petroleum and Petrochemical Engineering, Hakim Sabzevari University, Sabzevar, Iran

Abstract: (5837 Views)

Background: One of the medicinal fungi that has been used in traditional medicine for a long time is the Basidiomycete fungus Fomes fomentarius, which is widely distributed in Iran. Polysaccharides as one of the metabolites of this fungus have anti-inflammatory, anti-diabetic, antibacterial, antioxidant, and anti-cancer properties.
Materials & Methods: Optimization of independent variables of MgSO₄.7H₂O concentration, initial pH, yeast extract, and inoculum percentage to increase biomass and polysaccharide production of F. fomentarius was investigated using the Taguchi method. Then, the biological properties of the produced polysaccharide including antibacterial activity was investigated by bacterial colony counting method, antioxidant activity using DPPH free radical, and antiproliferative effect on 5 cancer cell lines MKN-45, AGS, A549, KYSE-30 and 5637 using MTS test.
Results: The concentration of MgSO₄.7H₂O and initial pH had a significant effect (P<0.05) on the production of F. fomentarius polysaccharide and in optimal conditions polysaccharide production reaches 5.410 g/L. The polysaccharide of this fungus inhibits the growth of Staphylococcus aureus and Escherichia coli bacteria by 50% and 25%, respectively. The antioxidant activity of this polysaccharide in the DPPH test is 16.11%. The antiproliferative effect of this polysaccharide on cancer cells is different (KYSE-30> A549

5637> AGS> MKN-45). This effect increases with increasing concentration. In KYSE-30 cell line treatment with 200 g/mL polysaccharide, cell viability reaches 40% after 72 hours.
Conclusion: Optimizing the culture medium of the medicinal fungus Fomes fomentarius increases the production of polysaccharides up to 5.410 g/L. Optimization increases the biological activity of polysaccharides. Antibacterial activity against Staphylococcus aureus and Escherichia coli is 50% and 25%, respectively. The antioxidant activity of polysaccharides is 16.11% and the viability of KYSE-30 cancer cells reaches 40% after 72 hours.

Keywords: Fomes fomentarius, Optimization, Taguchi, Antibacterial, Antioxidant, Anticancer

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Type of Study: Original Research Article | Subject: Microbial Biotechnology
Received: 2020/08/27 | Accepted: 2020/10/5 | ePublished: 2020/10/27

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