Alireza Salimi Chirani, Hossein Dabiri, Iraj Nikokar, Monireh Ebrahim Pour Komleh, Frahnoosh Dousdar, Hossein Goudarzi, Fatemeh Fallah, Ali Esmaili,
year 8, Issue 3 (10-2014)
Background
and Aim: Type IV pilin molecule in Pseudomonas aeruginosa with
wide functions, has important role in the diversity of the bacterial
genome. According to the latest
classification, Type IV pilin is divided to two main subtypes IVa, IVb. The
major subunit encoding Type IV pilin subtypes IVa, IVb is pilA and pilS2
genes respectively which serve as a marker for the detection of the Type IV
pilin subtypes. The purpose of the current study was to evaluate the frequency
of pilA and pilS2 gene in cystic fibrosis, burns, and
environmental isolates.
Materials
and Methods: The samples for P.aeruginosa were
collected from cystic fibrosis, burns, and environment wastewater during the
April 2013 to December 2013. Samples were cultured and identified using
microbial and biochemical methods. DNA of isolates was extracted by commercial
kit. PCR was performed using specific primers. Statistical analysis was done
using SPSS 17.0 software.
Results:
A
total of 100 P.aeruginosa isolates were collected 30 cystic fibrosis,
30 burn, 40 environmental. The prevalence of the pilA gene were 63.3% , 56.7% and 45/2% for
cystic fibrosis, for burns and
environmental strains . The PilS2 gene frequency was 57.5% of the
environmental isolates, 67.5% of cystic fibrosis strains and 73.3 % of the
isolates were burned.
Conclusions:
Our
study showed that the frequency of P.aeruginosa T4P major pilin subunits
in our P. aeruginosa population was high. The incidence of pilS2
gene was greater than pilA and was more frequent in Pseudomonas
aeruginosa isolated from hospitalized burn patients.
