Amirhossein Farshchi Tabrizi, Abolfazl Rafati Zomorodi, Farshad Kakian, Aida Moazemy, Leila Kasraian, Sita Nakhaeitazeji, Mohammad Motamedifar,
year 18, Issue 4 (July - August 2024)
Abstract
Background and Aim: Following genetic defects in cystic fibrosis (CF), mucus accumulation and hypoxic gradients develop in the lungs creating conditions for the anaerobic bacterial colonization. This study investigated the difference between the prevalence of some anaerobic bacteria and antibiotic resistance among healthy and CF groups.
Materials and Methods: In this case-control study, total RNA was extracted from blood samples of 40 GC patients with 29 H. pylori positive samples, and 40 healthy controls with 25 H. pylori positive individuals. The quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure the expression levels of hsa-mir-196 and hsa-mir-153.
Results: Statistical analysis revealed a significant upregulation of hsa-mir-196 and a significant downregulation of hsa-mir-153 in the GC patients with H. pylori infection compared to those without infection and healthy controls.
Conclusion: In-silico analysis demonstrated the association of hsa-mir-196 and hsa-mir-153 with GC. These miRNAs are potential biomarkers for the GC associated with H. pylori infection and can serve as diagnostic tools for the early detection and may have prognostic value in predicting disease progression. However, further validation in larger cohorts and functional studies are necessary to fully understand their role in the GC development and assess their potential as therapeutic targets.
