Showing 5 results for Heidari
Mohammad Feizabadi, Ahmadreza Bahrmand, Parvin Heidarieh, Hossein Shojaee,
year 1, Issue 4 (Winter 2008)
Abstract
Background and Objectives: Multi locus enzyme electrophoresis (MEE)has been proved to be a powerful
technique in population genetic studies and molecular epidemiology of pathogenic micro-organisms. In this
study MEE was used to determine the genetic relationships of M. kansasii strains cultured from patients at
Pasteur Institute of Iran.
Material and Methods: 21 isolates of M. kansasii (9 isolates from Iran and 12 isolates from other countries)
were analyzed for 12 enzymes loci by MEE. Isolate swere grown on LJ slants and BACTEC 13A. The cells
were sedimented by centrifugation and their lysates containing the enzymes were extracted by sonication.
The horizontal starch gel electrophoresis was used for visualization of enzymes after staining the gels with
substrate in solutions or agar overlay.
Results: A considerable genetic diversity was found at different loci of M. kansasii suggesting the existence
of different sub-species for this organism. It also showed the inaccuracy of some biochemical test for
identification of some isolates with in this species.
Conclusion: Iranian isolates of M. kansasii are genetically diverse. Separation of isolates at high genetic
distances in this study suggest the possible existence ofundetected isolates that could fill the gaps between
the unrelated isolates.
Mohammadreza Mahzonieh, Heidar Heidari Khoei, Mohammad Ghasemi Shamsabadi, Fatemeh Heidari,
year 7, Issue 1 (Spring 2013)
Abstract
Background and objectives: Chlamydia psittaci is an intracellular bacterium that may cause endemic avian chlamydios is and respiratory psittacosis in humans. Feral birds and domesticated poultry are considered as potential hosts. While feral pigeons in towns worldwide are commonly infected, infection may occur via inhalation of aerosols of dried infective avian excreta. The aim of this study was the determination of prevalence of C. psittaci in feces of pigeons in Chaharmahal va Bakhtiari and Yazd provinces in Iran using nested PCR.
Material and Methods: Samples were collected from pigeons in bird shops, backyards or cages in houses. Eighty eight genomic DNAs were extracted from the samples with a DNA purification kit (CinnaGen Inc. Iran) according to the manufacturer’s instructions. Extracted DNA was tested to detect C. psittaci DNA by a nested genus- and species-specific PCR. Primers were designed to amplify 16s rRNA. The first pair of primers was specific for the genus, and the second pair was specific to the species of C. psittaci.
Results: he average infection rate was about %52 (46 samples from 88 samples).
Conclusion:T It shows that a relatively high percentage of pigeons were infected with C. psittaci and may be able to play an important role as a source of infection for human or other mammals. More studies should be done to find more information like predominant genotypes of C. psittaci in Iran.
Khatoon Heidari, Hami Kaboosi, Ailar Jamali, Ezzat Allah Ghaemi, Fatemeh Peyravii Ghadikolaii,
year 13, Issue 1 (March - April 2019)
Abstract
Background and Aims: Helicobacter pylori is the main cause of various gastroduodenal diseases. It is estimated that app roximately, more than half of the adult population in developed countries and 90% of people in developing countries infected with H. pylori. H. pylori infection may be related to Genetic of virulence factors and environmental factors. The aim of this study was to assess of frequency cagA and vacA genes of H. pylori isolated from patients with Gastrointestinal Disorders.
Materials and Methods: This cross-sectional descriptive study carried out on 120 patients with gastrointestinal diseases in Gorgan city in 2017. (40 patients of gastric cancer, 40 patients of peptic ulcer, 40 patients of without cancer and ulcer). After genomic DNA extraction PCR was carried out using specific H.pylori primers.
Results: Overall, 120 H.pylori strains were isolated. The frequency of cagA was %67.5 in gastric cancer, %60 in peptic ulcer and %45 in patiens without ulcer and gastric cancer. Also frequency of vacA gene was detected %55 in gastric cancer, %40 in peptic ulcer and %27.5 in patiens without ulcer and gastric cancer.
Conclusion: Based on our findings it seems that the cag A and vac A genes were virulence among H. pylori isolated from studied patients. The frequency of cagA and vacA genes H. pylori were than in gastric cancer and peptic ulcer patients.
Reza Ansari, Payman Dabirmoghaddam, Maryam Lotfi, Mina Gheitani, Saeed Sohrabpour, Farrokh Heidari,
year 15, Issue 2 (March - April 2021)
Abstract
A 49-year-old woman with a history of diabetes mellitus (DM), and hypothyroidism referred to the emergency ward complaining of shortness of breath which had lasted for three weeks. Due to inspiratory and expiratory stridor in the clinical examination, a tracheal lesion was proposed for her. In computed tomography (CT) scan (without contrast) of the neck and chest, a lesion resembling a malignant tracheal tumor was observed spreading around the cervical trachea. Subsequently, the patient's respiratory distress worsened and she underwent tracheostomy under general anesthesia. During tracheostomy, a white to creamy lesion that resembled necrosis with extensive granulation was seen in cervical trachea, and a biopsy was taken. Histopathological reports showed evidence of acute and chronic inflammation, in necrotic background, along with aseptate fungi which confirmed mucormycosis. Initially, intravenous liposomal amphotericin-B was selected as an antifungal drug which was discontinued due to drug-induced acute renal failure. Posaconazole suspension was replaced as an antifungal drug. After about six weeks, the patient was discharged from the hospital in good general condition. Contrary to few previous studies on mucormycosis of the trachea and lower airways, tracheal disease was limited in our patient; therefore, we avoided debridement of the conflict site and tried to control the disease by controlling the underlying disease (DM), and antifungal therapy. Finally, the desired result was achieved. It should be noted that all patients who have been reviewed in the previous published studies have had a wider conflict sites compared to our patient. Therefore, due to the lack of standard treatment for this disease, our therapeutic approach in this study can be considered as an option in limited and localized cases.
Pegah Babaheidarian, Majid Mehdinejad, Ali Zare-Mirzaie, Roya Mokarinejad, Ensieh Jafari,
year 16, Issue 2 (March - April 2022)
Abstract
Background and Objective: A large group of genes associated with extended-spectrum beta-lactamases (ESBL) and AmpC expression is involved in inducing antibiotic resistance in various bacteria. Identifying these genes and assessing their harboring will be crucial in determining the pattern of antibiotic resistance. This study aimed to characterize genetic variants in extended-spectrum beta-lactamases, AmpC-producing gram-negative bacilli, and associated antibiotic resistance.
Methods: This cross-sectional research was conducted on clinical samples of patients admitted to Rasool-e-Akram Hospital, Tehran, Iran (2019 and 2020). The genetic variants were assessed by the Polymerase chain reaction method. The pattern of durability to antibiotics was determined by the disk diffusion system.
Results: Of the 80 isolates that produced ESBL and AmpC beta-lactamase, 75 cases were ESBL producers and 5 cases co-producers of ESBL and AmpC. The most common cultivated strains included Escherichia coli (61.2%) and Klebsiella pneumoniae (31.3%). The highest antibiotic resistance in ESBL producers was related to cefotaxime, trimethoprim-sulfamethoxazole, and cefazolin, and the lowest resistance level was related to colistin, cefoxitin, and ceftriaxone. The total frequencies of common genes of ESBL-producing gram-negative bacilli were blaCTX-M (76.0%), blaTEM (46.7%), and blaSHV (26.7%), while multigenic harboring was also observed in 49.3%. The level of drug resistance to Imipenem, Amikacin, and Ceftazidime in people who harbored the blaSHV gene was significantly higher than in other cases.
Conclusion: The most common cultivated strains included E. coli and K. pneumoniae. blaCTX-M, blaTEM, blaSHV, and multigenic expression were observed. The detection of the blaSHV gene is associated with increased antibiotic resistance to Imipenem, Amikacin, and Ceftazidime.
