year 12, Issue 5 (November - December 2018)                   Iran J Med Microbiol 2018, 12(5): 301-310 | Back to browse issues page


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Sedighian Kashi N, Honarmand S, Baghbani Arani F. Investigation of Association Between adeA, adeB ,adeI Genes and Phenotypic Activity of Efflux Pump in Multiple Drug Resistant Acinetobacter baumannii Isolates . Iran J Med Microbiol. 2018; 12 (5) :301-310
URL: http://ijmm.ir/article-1-868-en.html
1- Department of Microbiology, Faculty of Biological Sciences, Varamin- Pishva Branch, Islamic Azad University, Varamin, Iran
2- Department of Microbiology, Faculty of Biological Sciences, Varamin- Pishva Branch, Islamic Azad University, Varamin, Iran , sahar_hj2@yahoo.com
3- Department of Genetics and Biotechnology, Faculty of Biological Sciences, Varamin- Pishva Branch, Islamic Azad University, Varamin, Iran
Abstract:   (4624 Views)
Background and Aims: Acinetobacter baumannii  is a major cause of nosocomial infections and is resistant to many antibiotics. Over expression of AdeABC and AdeIJK efflux pumps in Acinetobacter causes resistance to aminoglycosides and decreases the sensitivity of fluoroquinolones. The aim of this study was to investigate the phenotypic activity of the Acinetobacter baumanni  isolates associated with  presence of adeA, adeB, and adeI genes.
Materials and Methods: The study was performed on 55 strains of A. baumannii isolated collected from specimens of patients hospitalized in Milad Hospital , Tehran. The isolates were diagnosed using biochemical tests and antibiotic susceptibility testing was done by disk diffusion method based on CLSI guidelines. Cartwheel method was used to study phenotypic activity of efflux pump. Multiplex PCR was used to determine the presence of genes.
Results: The prevalence of multiple drug resistant isolates was 98%. In terms of efflux pump activity 3.63% isolates were strong, 67.27% moderate and 29.09% were non-active.  The frequency of adeA, adeB, adeI genes was 87.2%, 85.4% and 94.5%, respectively. There was significant association between adeA and adeB genes among isolates with actively and non-actively efflux pump.
 Conclusions: Determination of actively phenotype of efflux pump can determine the multiple drug resistance among A. baumannii isolates. The results confirms the role of main genes encoding AdeABC operon,adeA and adeB  in activity of A. baumannii efflux pump.
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Type of Study: Original Research Article | Subject: Molecular Microbiology
Received: 2018/08/25 | Accepted: 2018/11/4 | ePublished: 2019/01/30

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